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Fabrication of laser printed microfluidic paper-based analytical devices (LP-µPADs) for point-of-care applications
Microfluidic paper-based analytical devices (µPADs) have provided a breakthrough in portable and low-cost point-of-care diagnostics. Despite their significant scope, the complexity of fabrication and reliance on expensive and sophisticated tools, have limited their outreach and possibility of commer...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6536539/ https://www.ncbi.nlm.nih.gov/pubmed/31133720 http://dx.doi.org/10.1038/s41598-019-44455-1 |
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author | Ghosh, Rajesh Gopalakrishnan, Saranya Savitha, Rangasamy Renganathan, Thiruvengadam Pushpavanam, Subramanium |
author_facet | Ghosh, Rajesh Gopalakrishnan, Saranya Savitha, Rangasamy Renganathan, Thiruvengadam Pushpavanam, Subramanium |
author_sort | Ghosh, Rajesh |
collection | PubMed |
description | Microfluidic paper-based analytical devices (µPADs) have provided a breakthrough in portable and low-cost point-of-care diagnostics. Despite their significant scope, the complexity of fabrication and reliance on expensive and sophisticated tools, have limited their outreach and possibility of commercialization. Herein, we report for the first time, a facile method to fabricate µPADs using a commonly available laser printer which drastically reduces the cost and complexity of fabrication. Toner ink is used to pattern the µPADs by printing, without modifying any factory configuration of the laser printer. Hydrophobic barriers are created by heating the patterned paper which melts the toner ink, facilitating its wicking into the cross-section of the substrate. Further, we demonstrate the utilization of the fabricated device by performing two assays. The proposed technique provides a versatile platform for rapid prototyping of µPADs with significant prospect in both developed and resource constrained region. |
format | Online Article Text |
id | pubmed-6536539 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-65365392019-06-06 Fabrication of laser printed microfluidic paper-based analytical devices (LP-µPADs) for point-of-care applications Ghosh, Rajesh Gopalakrishnan, Saranya Savitha, Rangasamy Renganathan, Thiruvengadam Pushpavanam, Subramanium Sci Rep Article Microfluidic paper-based analytical devices (µPADs) have provided a breakthrough in portable and low-cost point-of-care diagnostics. Despite their significant scope, the complexity of fabrication and reliance on expensive and sophisticated tools, have limited their outreach and possibility of commercialization. Herein, we report for the first time, a facile method to fabricate µPADs using a commonly available laser printer which drastically reduces the cost and complexity of fabrication. Toner ink is used to pattern the µPADs by printing, without modifying any factory configuration of the laser printer. Hydrophobic barriers are created by heating the patterned paper which melts the toner ink, facilitating its wicking into the cross-section of the substrate. Further, we demonstrate the utilization of the fabricated device by performing two assays. The proposed technique provides a versatile platform for rapid prototyping of µPADs with significant prospect in both developed and resource constrained region. Nature Publishing Group UK 2019-05-27 /pmc/articles/PMC6536539/ /pubmed/31133720 http://dx.doi.org/10.1038/s41598-019-44455-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ghosh, Rajesh Gopalakrishnan, Saranya Savitha, Rangasamy Renganathan, Thiruvengadam Pushpavanam, Subramanium Fabrication of laser printed microfluidic paper-based analytical devices (LP-µPADs) for point-of-care applications |
title | Fabrication of laser printed microfluidic paper-based analytical devices (LP-µPADs) for point-of-care applications |
title_full | Fabrication of laser printed microfluidic paper-based analytical devices (LP-µPADs) for point-of-care applications |
title_fullStr | Fabrication of laser printed microfluidic paper-based analytical devices (LP-µPADs) for point-of-care applications |
title_full_unstemmed | Fabrication of laser printed microfluidic paper-based analytical devices (LP-µPADs) for point-of-care applications |
title_short | Fabrication of laser printed microfluidic paper-based analytical devices (LP-µPADs) for point-of-care applications |
title_sort | fabrication of laser printed microfluidic paper-based analytical devices (lp-µpads) for point-of-care applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6536539/ https://www.ncbi.nlm.nih.gov/pubmed/31133720 http://dx.doi.org/10.1038/s41598-019-44455-1 |
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