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Detection of Influenza a Virus in Swine Nasal Swab Samples With a Wash-Free Magnetic Bioassay and a Handheld Giant Magnetoresistance Sensing System

The dissemination of Influenza A virus (IAV) throughout the world has become one of the main concerns for the health of both animals and human beings. An efficient and sensitive diagnostic tool is thus needed for the early detection of IAV. Here, we developed a wash-free magnetic bioassay and furthe...

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Autores principales: Su, Diqing, Wu, Kai, Krishna, Venkatramana D., Klein, Todd, Liu, Jinming, Feng, Yinglong, Perez, Andres M., Cheeran, Maxim C.-J., Wang, Jian-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6536586/
https://www.ncbi.nlm.nih.gov/pubmed/31164877
http://dx.doi.org/10.3389/fmicb.2019.01077
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author Su, Diqing
Wu, Kai
Krishna, Venkatramana D.
Klein, Todd
Liu, Jinming
Feng, Yinglong
Perez, Andres M.
Cheeran, Maxim C.-J.
Wang, Jian-Ping
author_facet Su, Diqing
Wu, Kai
Krishna, Venkatramana D.
Klein, Todd
Liu, Jinming
Feng, Yinglong
Perez, Andres M.
Cheeran, Maxim C.-J.
Wang, Jian-Ping
author_sort Su, Diqing
collection PubMed
description The dissemination of Influenza A virus (IAV) throughout the world has become one of the main concerns for the health of both animals and human beings. An efficient and sensitive diagnostic tool is thus needed for the early detection of IAV. Here, we developed a wash-free magnetic bioassay and further integrated it with a handheld platform based on giant-magnetoresistance (GMR) sensors. The wash-free magnetic bioassay significantly accelerates and simplifies the detection process. This brand-new system was successful in detecting both IAV nucleoprotein and IAV-contained nasal swab samples from pigs on the farm. The limit of detection (LOD) is 0.3 nM for IAV nucleoprotein and 250 TCID(50)/mL for IAV-spiked nasal swab samples. The detection of nasal swab samples containing unpurified IAV was also performed, demonstrating the capability of the magnetic wash-free assay in the detection of biomarkers in complex sample matrix.
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spelling pubmed-65365862019-06-04 Detection of Influenza a Virus in Swine Nasal Swab Samples With a Wash-Free Magnetic Bioassay and a Handheld Giant Magnetoresistance Sensing System Su, Diqing Wu, Kai Krishna, Venkatramana D. Klein, Todd Liu, Jinming Feng, Yinglong Perez, Andres M. Cheeran, Maxim C.-J. Wang, Jian-Ping Front Microbiol Microbiology The dissemination of Influenza A virus (IAV) throughout the world has become one of the main concerns for the health of both animals and human beings. An efficient and sensitive diagnostic tool is thus needed for the early detection of IAV. Here, we developed a wash-free magnetic bioassay and further integrated it with a handheld platform based on giant-magnetoresistance (GMR) sensors. The wash-free magnetic bioassay significantly accelerates and simplifies the detection process. This brand-new system was successful in detecting both IAV nucleoprotein and IAV-contained nasal swab samples from pigs on the farm. The limit of detection (LOD) is 0.3 nM for IAV nucleoprotein and 250 TCID(50)/mL for IAV-spiked nasal swab samples. The detection of nasal swab samples containing unpurified IAV was also performed, demonstrating the capability of the magnetic wash-free assay in the detection of biomarkers in complex sample matrix. Frontiers Media S.A. 2019-05-21 /pmc/articles/PMC6536586/ /pubmed/31164877 http://dx.doi.org/10.3389/fmicb.2019.01077 Text en Copyright © 2019 Su, Wu, Krishna, Klein, Liu, Feng, Perez, Cheeran and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Su, Diqing
Wu, Kai
Krishna, Venkatramana D.
Klein, Todd
Liu, Jinming
Feng, Yinglong
Perez, Andres M.
Cheeran, Maxim C.-J.
Wang, Jian-Ping
Detection of Influenza a Virus in Swine Nasal Swab Samples With a Wash-Free Magnetic Bioassay and a Handheld Giant Magnetoresistance Sensing System
title Detection of Influenza a Virus in Swine Nasal Swab Samples With a Wash-Free Magnetic Bioassay and a Handheld Giant Magnetoresistance Sensing System
title_full Detection of Influenza a Virus in Swine Nasal Swab Samples With a Wash-Free Magnetic Bioassay and a Handheld Giant Magnetoresistance Sensing System
title_fullStr Detection of Influenza a Virus in Swine Nasal Swab Samples With a Wash-Free Magnetic Bioassay and a Handheld Giant Magnetoresistance Sensing System
title_full_unstemmed Detection of Influenza a Virus in Swine Nasal Swab Samples With a Wash-Free Magnetic Bioassay and a Handheld Giant Magnetoresistance Sensing System
title_short Detection of Influenza a Virus in Swine Nasal Swab Samples With a Wash-Free Magnetic Bioassay and a Handheld Giant Magnetoresistance Sensing System
title_sort detection of influenza a virus in swine nasal swab samples with a wash-free magnetic bioassay and a handheld giant magnetoresistance sensing system
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6536586/
https://www.ncbi.nlm.nih.gov/pubmed/31164877
http://dx.doi.org/10.3389/fmicb.2019.01077
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