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The potential chemo preventive effect of ursolic acid isolated from Paulownia tomentosa, against N-diethylnitrosamine: initiated and promoted hepatocarcinogenesis

The present study discusses the isolation of ursolic acid from the chloroform extract of Paulownia tomentosa (Thunb) Steud fruits and its cytotoxic effect has been assessed in-vitro was performed in different cells lines (A-549, MCF-7, HepG2) and in-vivo using N-diethylnitrosamine. The obtained resu...

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Autores principales: Ali, Sanaa A., Ibrahim, Nabaweya A., Mohammed, Magdy M.D., El-Hawary, Seham, Refaat, Esraa A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6536727/
https://www.ncbi.nlm.nih.gov/pubmed/31193530
http://dx.doi.org/10.1016/j.heliyon.2019.e01769
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author Ali, Sanaa A.
Ibrahim, Nabaweya A.
Mohammed, Magdy M.D.
El-Hawary, Seham
Refaat, Esraa A.
author_facet Ali, Sanaa A.
Ibrahim, Nabaweya A.
Mohammed, Magdy M.D.
El-Hawary, Seham
Refaat, Esraa A.
author_sort Ali, Sanaa A.
collection PubMed
description The present study discusses the isolation of ursolic acid from the chloroform extract of Paulownia tomentosa (Thunb) Steud fruits and its cytotoxic effect has been assessed in-vitro was performed in different cells lines (A-549, MCF-7, HepG2) and in-vivo using N-diethylnitrosamine. The obtained results revealed that ursolic acid showed significant cytotoxic activity on MCF-7 and HepG2 cell lines in comparison to Doxorubicin as a reference drug. Moreover, we have assessed the inhibitory effects of Paulownia tomentosa fruit chloroform extract and the isolated ursolic acid on hepatocarcinogenesis was carried out for the first time using N-diethylnitrosamine, where the group treated with ursolic acid given orally after 8 weeks of cancer induction showed the most significant results in comparison to the chloroform extract. The effect of ursolic acid on intoxicated rats caused significant restoration of most of the normal hepatocytes architecture with regular dark nuclei and the group treated with Paulownia tomentosa fruits showed remarkable results with improvement in biochemical analysis.
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spelling pubmed-65367272019-05-30 The potential chemo preventive effect of ursolic acid isolated from Paulownia tomentosa, against N-diethylnitrosamine: initiated and promoted hepatocarcinogenesis Ali, Sanaa A. Ibrahim, Nabaweya A. Mohammed, Magdy M.D. El-Hawary, Seham Refaat, Esraa A. Heliyon Article The present study discusses the isolation of ursolic acid from the chloroform extract of Paulownia tomentosa (Thunb) Steud fruits and its cytotoxic effect has been assessed in-vitro was performed in different cells lines (A-549, MCF-7, HepG2) and in-vivo using N-diethylnitrosamine. The obtained results revealed that ursolic acid showed significant cytotoxic activity on MCF-7 and HepG2 cell lines in comparison to Doxorubicin as a reference drug. Moreover, we have assessed the inhibitory effects of Paulownia tomentosa fruit chloroform extract and the isolated ursolic acid on hepatocarcinogenesis was carried out for the first time using N-diethylnitrosamine, where the group treated with ursolic acid given orally after 8 weeks of cancer induction showed the most significant results in comparison to the chloroform extract. The effect of ursolic acid on intoxicated rats caused significant restoration of most of the normal hepatocytes architecture with regular dark nuclei and the group treated with Paulownia tomentosa fruits showed remarkable results with improvement in biochemical analysis. Elsevier 2019-05-24 /pmc/articles/PMC6536727/ /pubmed/31193530 http://dx.doi.org/10.1016/j.heliyon.2019.e01769 Text en © 2019 Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Ali, Sanaa A.
Ibrahim, Nabaweya A.
Mohammed, Magdy M.D.
El-Hawary, Seham
Refaat, Esraa A.
The potential chemo preventive effect of ursolic acid isolated from Paulownia tomentosa, against N-diethylnitrosamine: initiated and promoted hepatocarcinogenesis
title The potential chemo preventive effect of ursolic acid isolated from Paulownia tomentosa, against N-diethylnitrosamine: initiated and promoted hepatocarcinogenesis
title_full The potential chemo preventive effect of ursolic acid isolated from Paulownia tomentosa, against N-diethylnitrosamine: initiated and promoted hepatocarcinogenesis
title_fullStr The potential chemo preventive effect of ursolic acid isolated from Paulownia tomentosa, against N-diethylnitrosamine: initiated and promoted hepatocarcinogenesis
title_full_unstemmed The potential chemo preventive effect of ursolic acid isolated from Paulownia tomentosa, against N-diethylnitrosamine: initiated and promoted hepatocarcinogenesis
title_short The potential chemo preventive effect of ursolic acid isolated from Paulownia tomentosa, against N-diethylnitrosamine: initiated and promoted hepatocarcinogenesis
title_sort potential chemo preventive effect of ursolic acid isolated from paulownia tomentosa, against n-diethylnitrosamine: initiated and promoted hepatocarcinogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6536727/
https://www.ncbi.nlm.nih.gov/pubmed/31193530
http://dx.doi.org/10.1016/j.heliyon.2019.e01769
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