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BR deficiency causes increased sensitivity to drought and yield penalty in cotton

BACKGROUND: Brassinosteroids (BRs) play crucial roles in drought tolerance, but the underlying molecular mechanisms remain unclear in the important oilseed and fiber crop, cotton (Gossypium hirsutum L.). RESULTS: To elucidate how BRs mediate drought tolerance in cotton, a cotton brassinosteroid (BR)...

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Autores principales: Chen, Eryong, Zhang, Xueyan, Yang, Zuoren, Zhang, Chaojun, Wang, Xiaoqian, Ge, Xiaoyang, Li, Fuguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6537406/
https://www.ncbi.nlm.nih.gov/pubmed/31138186
http://dx.doi.org/10.1186/s12870-019-1832-9
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author Chen, Eryong
Zhang, Xueyan
Yang, Zuoren
Zhang, Chaojun
Wang, Xiaoqian
Ge, Xiaoyang
Li, Fuguang
author_facet Chen, Eryong
Zhang, Xueyan
Yang, Zuoren
Zhang, Chaojun
Wang, Xiaoqian
Ge, Xiaoyang
Li, Fuguang
author_sort Chen, Eryong
collection PubMed
description BACKGROUND: Brassinosteroids (BRs) play crucial roles in drought tolerance, but the underlying molecular mechanisms remain unclear in the important oilseed and fiber crop, cotton (Gossypium hirsutum L.). RESULTS: To elucidate how BRs mediate drought tolerance in cotton, a cotton brassinosteroid (BR)-deficient mutant, pag1 (pagoda1), was employed for analysis. Importantly, the pag1 mutant showed increased sensitivity to drought stress, with shorter primary roots and fewer lateral roots. The number of stomata was significantly increased in the mutant, and the stomata aperture was much wider than that of the control plants. These mutant plants therefore showed an increased water loss rate. Furthermore, the abscisic acid (ABA) content, photosynthetic efficiency and starch content of the mutant were significantly lower than those of the wild type. The overall performance of the mutant plants was worse than that of the wild-type control under both normal and drought conditions. Moreover, Proteomic analysis revealed reduced levels of stress-related proteins in pag1 plants. CONCLUSIONS: These results suggest that BRs may modulate the drought tolerance of cotton by regulating much genes that related to drought stress and multiple organ responses to drought, including root growth, stomata development, the stomata aperture and photosynthesis. This study provides an important basis for understanding drought resistance regulated by BRs and cultivating drought-resistant cotton lines. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-019-1832-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-65374062019-05-30 BR deficiency causes increased sensitivity to drought and yield penalty in cotton Chen, Eryong Zhang, Xueyan Yang, Zuoren Zhang, Chaojun Wang, Xiaoqian Ge, Xiaoyang Li, Fuguang BMC Plant Biol Research Article BACKGROUND: Brassinosteroids (BRs) play crucial roles in drought tolerance, but the underlying molecular mechanisms remain unclear in the important oilseed and fiber crop, cotton (Gossypium hirsutum L.). RESULTS: To elucidate how BRs mediate drought tolerance in cotton, a cotton brassinosteroid (BR)-deficient mutant, pag1 (pagoda1), was employed for analysis. Importantly, the pag1 mutant showed increased sensitivity to drought stress, with shorter primary roots and fewer lateral roots. The number of stomata was significantly increased in the mutant, and the stomata aperture was much wider than that of the control plants. These mutant plants therefore showed an increased water loss rate. Furthermore, the abscisic acid (ABA) content, photosynthetic efficiency and starch content of the mutant were significantly lower than those of the wild type. The overall performance of the mutant plants was worse than that of the wild-type control under both normal and drought conditions. Moreover, Proteomic analysis revealed reduced levels of stress-related proteins in pag1 plants. CONCLUSIONS: These results suggest that BRs may modulate the drought tolerance of cotton by regulating much genes that related to drought stress and multiple organ responses to drought, including root growth, stomata development, the stomata aperture and photosynthesis. This study provides an important basis for understanding drought resistance regulated by BRs and cultivating drought-resistant cotton lines. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-019-1832-9) contains supplementary material, which is available to authorized users. BioMed Central 2019-05-28 /pmc/articles/PMC6537406/ /pubmed/31138186 http://dx.doi.org/10.1186/s12870-019-1832-9 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Chen, Eryong
Zhang, Xueyan
Yang, Zuoren
Zhang, Chaojun
Wang, Xiaoqian
Ge, Xiaoyang
Li, Fuguang
BR deficiency causes increased sensitivity to drought and yield penalty in cotton
title BR deficiency causes increased sensitivity to drought and yield penalty in cotton
title_full BR deficiency causes increased sensitivity to drought and yield penalty in cotton
title_fullStr BR deficiency causes increased sensitivity to drought and yield penalty in cotton
title_full_unstemmed BR deficiency causes increased sensitivity to drought and yield penalty in cotton
title_short BR deficiency causes increased sensitivity to drought and yield penalty in cotton
title_sort br deficiency causes increased sensitivity to drought and yield penalty in cotton
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6537406/
https://www.ncbi.nlm.nih.gov/pubmed/31138186
http://dx.doi.org/10.1186/s12870-019-1832-9
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