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Molecular Characterization and Biological Function of a Novel LncRNA CRNG in Swine

Our previous study has showed that a novel gene is differentially expressed in the liver of cyadox-fed piglets, but its sequence and function are unknown. Here, rapid amplification of cDNA ends (RACE) and bioinformatics analysis showed that the novel gene is 953 bp without protein-coding ability and...

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Autores principales: Lu, Qirong, Li, Li, Huang, Aixin, Cui, Luqing, Zhang, Yinfeng, Liu, Qianying, Wang, Xu, Wang, Yulian, Liu, Zhenli, Yuan, Zonghui, Dai, Menghong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6537671/
https://www.ncbi.nlm.nih.gov/pubmed/31178726
http://dx.doi.org/10.3389/fphar.2019.00539
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author Lu, Qirong
Li, Li
Huang, Aixin
Cui, Luqing
Zhang, Yinfeng
Liu, Qianying
Wang, Xu
Wang, Yulian
Liu, Zhenli
Yuan, Zonghui
Dai, Menghong
author_facet Lu, Qirong
Li, Li
Huang, Aixin
Cui, Luqing
Zhang, Yinfeng
Liu, Qianying
Wang, Xu
Wang, Yulian
Liu, Zhenli
Yuan, Zonghui
Dai, Menghong
author_sort Lu, Qirong
collection PubMed
description Our previous study has showed that a novel gene is differentially expressed in the liver of cyadox-fed piglets, but its sequence and function are unknown. Here, rapid amplification of cDNA ends (RACE) and bioinformatics analysis showed that the novel gene is 953 bp without protein-coding ability and locates in chromosome 11. Hence, we identified the novel gene as long non-coding RNA (lncRNA) and named it cyadox-related novel gene (CRNG). Fluorescence in situ hybridization (FISH) showed that CRNG mainly distributes in cytoplasm. Moreover, microarray assay in combination with CRNG interference and overexpression showed that the differential genes such as ANPEP, KITLG, STAT5A, FOXP3, miR-451, IL-2, IL-10, IL-6, and TNF-α are mainly involved in viral and pathogens infection and the immune-inflammatory responses in PK-15 cells. This work reveals that CRNG might play a role in preventing the host from being infected by pathogens and viruses and exerting immune regulatory effects in the cytoplasm, which may be involved in prophylaxis of cyadox in piglets.
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spelling pubmed-65376712019-06-07 Molecular Characterization and Biological Function of a Novel LncRNA CRNG in Swine Lu, Qirong Li, Li Huang, Aixin Cui, Luqing Zhang, Yinfeng Liu, Qianying Wang, Xu Wang, Yulian Liu, Zhenli Yuan, Zonghui Dai, Menghong Front Pharmacol Pharmacology Our previous study has showed that a novel gene is differentially expressed in the liver of cyadox-fed piglets, but its sequence and function are unknown. Here, rapid amplification of cDNA ends (RACE) and bioinformatics analysis showed that the novel gene is 953 bp without protein-coding ability and locates in chromosome 11. Hence, we identified the novel gene as long non-coding RNA (lncRNA) and named it cyadox-related novel gene (CRNG). Fluorescence in situ hybridization (FISH) showed that CRNG mainly distributes in cytoplasm. Moreover, microarray assay in combination with CRNG interference and overexpression showed that the differential genes such as ANPEP, KITLG, STAT5A, FOXP3, miR-451, IL-2, IL-10, IL-6, and TNF-α are mainly involved in viral and pathogens infection and the immune-inflammatory responses in PK-15 cells. This work reveals that CRNG might play a role in preventing the host from being infected by pathogens and viruses and exerting immune regulatory effects in the cytoplasm, which may be involved in prophylaxis of cyadox in piglets. Frontiers Media S.A. 2019-05-21 /pmc/articles/PMC6537671/ /pubmed/31178726 http://dx.doi.org/10.3389/fphar.2019.00539 Text en Copyright © 2019 Lu, Li, Huang, Cui, Zhang, Liu, Wang, Wang, Liu, Yuan and Dai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Lu, Qirong
Li, Li
Huang, Aixin
Cui, Luqing
Zhang, Yinfeng
Liu, Qianying
Wang, Xu
Wang, Yulian
Liu, Zhenli
Yuan, Zonghui
Dai, Menghong
Molecular Characterization and Biological Function of a Novel LncRNA CRNG in Swine
title Molecular Characterization and Biological Function of a Novel LncRNA CRNG in Swine
title_full Molecular Characterization and Biological Function of a Novel LncRNA CRNG in Swine
title_fullStr Molecular Characterization and Biological Function of a Novel LncRNA CRNG in Swine
title_full_unstemmed Molecular Characterization and Biological Function of a Novel LncRNA CRNG in Swine
title_short Molecular Characterization and Biological Function of a Novel LncRNA CRNG in Swine
title_sort molecular characterization and biological function of a novel lncrna crng in swine
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6537671/
https://www.ncbi.nlm.nih.gov/pubmed/31178726
http://dx.doi.org/10.3389/fphar.2019.00539
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