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Improved preimplantation development of porcine somatic cell nuclear transfer embryos by caffeine treatment
This study examined the effects of a caffeine treatment to improve nuclear reprogramming in porcine cloned embryos. Embryonic development and the expression of genes related to pluripotency (POU5F1, SOX2, NANOG, and CDX2) were compared after caffeine supplementation during manipulation at different...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Veterinary Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6538509/ https://www.ncbi.nlm.nih.gov/pubmed/31161749 http://dx.doi.org/10.4142/jvs.2019.20.e31 |
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author | Kim, Ghangyong Roy, Pantu Kumar Fang, Xun Hassan, Bahia MS Cho, Jongki |
author_facet | Kim, Ghangyong Roy, Pantu Kumar Fang, Xun Hassan, Bahia MS Cho, Jongki |
author_sort | Kim, Ghangyong |
collection | PubMed |
description | This study examined the effects of a caffeine treatment to improve nuclear reprogramming in porcine cloned embryos. Embryonic development and the expression of genes related to pluripotency (POU5F1, SOX2, NANOG, and CDX2) were compared after caffeine supplementation during manipulation at different concentrations (0, 1.25, 2.5, and 5.0 mM) and after varying the delayed activation time (control, 1, 2, and 4 h) after fusion. Caffeine added to media during manipulation produced a higher rate of development to blastocysts in the 1.25 mM group than in the other concentration groups (22.8% vs. 16.1%, 16.2%, and 19.2%; p < 0.05). When caffeine was added during the 4 h delayed activation, the 1.25 mM caffeine concentration produced a significantly higher rate of development than those in the other 4 h-activation-delayed caffeine concentration groups (22.4% vs. 9.4%, 14.0%, and 11.1%; p < 0.05). On the other hand, no significant improvement over that in the control group was observed when caffeine was supplemented during both the manipulation period and delayed activation period (16.0% vs. 15.2%), respectively. The levels of POU5F1, SOX2, and NANOG expression in blastocysts were significantly higher in the delayed activation caffeine group (4 h, 1.25 mM) than in the control group (1 h, 0 mM; p < 0.05). In conclusion, a caffeine treatment at 1.25 mM during delayed activation for 4 h can improve the preimplantation development of porcine somatic cell nuclear transfer embryos by activating nuclear reprogramming. |
format | Online Article Text |
id | pubmed-6538509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-65385092019-06-04 Improved preimplantation development of porcine somatic cell nuclear transfer embryos by caffeine treatment Kim, Ghangyong Roy, Pantu Kumar Fang, Xun Hassan, Bahia MS Cho, Jongki J Vet Sci Original Article This study examined the effects of a caffeine treatment to improve nuclear reprogramming in porcine cloned embryos. Embryonic development and the expression of genes related to pluripotency (POU5F1, SOX2, NANOG, and CDX2) were compared after caffeine supplementation during manipulation at different concentrations (0, 1.25, 2.5, and 5.0 mM) and after varying the delayed activation time (control, 1, 2, and 4 h) after fusion. Caffeine added to media during manipulation produced a higher rate of development to blastocysts in the 1.25 mM group than in the other concentration groups (22.8% vs. 16.1%, 16.2%, and 19.2%; p < 0.05). When caffeine was added during the 4 h delayed activation, the 1.25 mM caffeine concentration produced a significantly higher rate of development than those in the other 4 h-activation-delayed caffeine concentration groups (22.4% vs. 9.4%, 14.0%, and 11.1%; p < 0.05). On the other hand, no significant improvement over that in the control group was observed when caffeine was supplemented during both the manipulation period and delayed activation period (16.0% vs. 15.2%), respectively. The levels of POU5F1, SOX2, and NANOG expression in blastocysts were significantly higher in the delayed activation caffeine group (4 h, 1.25 mM) than in the control group (1 h, 0 mM; p < 0.05). In conclusion, a caffeine treatment at 1.25 mM during delayed activation for 4 h can improve the preimplantation development of porcine somatic cell nuclear transfer embryos by activating nuclear reprogramming. The Korean Society of Veterinary Science 2019-05 2019-05-20 /pmc/articles/PMC6538509/ /pubmed/31161749 http://dx.doi.org/10.4142/jvs.2019.20.e31 Text en © 2019 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Ghangyong Roy, Pantu Kumar Fang, Xun Hassan, Bahia MS Cho, Jongki Improved preimplantation development of porcine somatic cell nuclear transfer embryos by caffeine treatment |
title | Improved preimplantation development of porcine somatic cell nuclear transfer embryos by caffeine treatment |
title_full | Improved preimplantation development of porcine somatic cell nuclear transfer embryos by caffeine treatment |
title_fullStr | Improved preimplantation development of porcine somatic cell nuclear transfer embryos by caffeine treatment |
title_full_unstemmed | Improved preimplantation development of porcine somatic cell nuclear transfer embryos by caffeine treatment |
title_short | Improved preimplantation development of porcine somatic cell nuclear transfer embryos by caffeine treatment |
title_sort | improved preimplantation development of porcine somatic cell nuclear transfer embryos by caffeine treatment |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6538509/ https://www.ncbi.nlm.nih.gov/pubmed/31161749 http://dx.doi.org/10.4142/jvs.2019.20.e31 |
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