The length of guide RNA and target DNA heteroduplex effects on CRISPR/Cas9 mediated genome editing efficiency in porcine cells

The clustered regularly interspaced short palindrome repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a versatile genome editing tool with high efficiency. A guide sequence of 20 nucleotides (nt) is commonly used in application of CRISPR/Cas9; however, the relationship between the lengt...

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Detalles Bibliográficos
Autores principales: Lv, Jiawei, Wu, Shuang, Wei, Renyue, Li, Yan, Jin, Junxue, Mu, Yanshuang, Zhang, Yu, Kong, Qingran, Weng, Xiaogang, Liu, Zhonghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2019
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6538514/
https://www.ncbi.nlm.nih.gov/pubmed/31161741
http://dx.doi.org/10.4142/jvs.2019.20.e23

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