Cargando…
In vivo microdialysis and in vitro HPLC analysis of the impact of paeoniflorin on the monoamine levels and their metabolites in the rodent brain
Background: Paeoniflorin (PF) possesses several effects such as analgesic, the anti-spasmodic effect on smooth muscle. It protects the cardiovascular system and reveals the neuroprotective effect on cerebral ischemia. Monoamine system has been identified to have complex regulatory effects in pain si...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
EDP Sciences
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6538943/ https://www.ncbi.nlm.nih.gov/pubmed/31124457 http://dx.doi.org/10.1051/bmdcn/2019090211 |
_version_ | 1783422267723612160 |
---|---|
author | Lin, Yuh-Tzy Huang, Wei-Shih Tsai, Huei-Yann Lee, Min-Min Chen, Yuh-Fung |
author_facet | Lin, Yuh-Tzy Huang, Wei-Shih Tsai, Huei-Yann Lee, Min-Min Chen, Yuh-Fung |
author_sort | Lin, Yuh-Tzy |
collection | PubMed |
description | Background: Paeoniflorin (PF) possesses several effects such as analgesic, the anti-spasmodic effect on smooth muscle. It protects the cardiovascular system and reveals the neuroprotective effect on cerebral ischemia. Monoamine system has been identified to have complex regulatory effects in pain signaling. There are no reports regarding the impact of PF on monoamine levels in the rodent brain by microdialysis. In this study, the effects of PF on monoamines and their metabolites in the rodent brain using in vivo microdialysis and in vitro high performance liquid chromatography (HPLC) analysis. Methods: Male S.D. rats were anesthetized, fixed onto the stereotaxic instrument to identify the positions of corpus striatum and cerebral cortex. Drilled a hole in the skull of anesthetic rats and proceeded microdialysis, and gave PF (100 μg, i.c.v.). Collected the dialysate and the concentration of monoamines and their metabolites in dialysate and analyzed with HPLC-ECD. Male ICR mice were administered with PF (96 μg, i.c.v.) and with Ringer solution as a control. After 20 mins of administration, the mice were cut off the brain immediately and separated into eight regions according to the method of Glowinski. Added extraction solution to each region, homogenized and extracted for further procedure. The extract was centrifuged, sucked the transparent layer and centrifuged once more. The transparent layer was filtered with a 0.22 μm nylon filter and analyzed with HPLC-ECD (electrochemical detection). Results: PF increased the content of DOPAC and NE in the cortex, and increased the content of NE and decreased the content of 5-HT in the medulla of the homogenized mice brain tissue. By microdialysis, PF increased the content of DOPAC and 5-HIAA in anesthetic rat cortex and expanded the content of DOPAC, HVA, and 5-HIAA in anesthetic rat striatum. Conclusions: It reveals that PF could activate the release of monoamines and increase their metabolites in the rodent brain. |
format | Online Article Text |
id | pubmed-6538943 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-65389432019-06-10 In vivo microdialysis and in vitro HPLC analysis of the impact of paeoniflorin on the monoamine levels and their metabolites in the rodent brain Lin, Yuh-Tzy Huang, Wei-Shih Tsai, Huei-Yann Lee, Min-Min Chen, Yuh-Fung Biomedicine (Taipei) Original Article Background: Paeoniflorin (PF) possesses several effects such as analgesic, the anti-spasmodic effect on smooth muscle. It protects the cardiovascular system and reveals the neuroprotective effect on cerebral ischemia. Monoamine system has been identified to have complex regulatory effects in pain signaling. There are no reports regarding the impact of PF on monoamine levels in the rodent brain by microdialysis. In this study, the effects of PF on monoamines and their metabolites in the rodent brain using in vivo microdialysis and in vitro high performance liquid chromatography (HPLC) analysis. Methods: Male S.D. rats were anesthetized, fixed onto the stereotaxic instrument to identify the positions of corpus striatum and cerebral cortex. Drilled a hole in the skull of anesthetic rats and proceeded microdialysis, and gave PF (100 μg, i.c.v.). Collected the dialysate and the concentration of monoamines and their metabolites in dialysate and analyzed with HPLC-ECD. Male ICR mice were administered with PF (96 μg, i.c.v.) and with Ringer solution as a control. After 20 mins of administration, the mice were cut off the brain immediately and separated into eight regions according to the method of Glowinski. Added extraction solution to each region, homogenized and extracted for further procedure. The extract was centrifuged, sucked the transparent layer and centrifuged once more. The transparent layer was filtered with a 0.22 μm nylon filter and analyzed with HPLC-ECD (electrochemical detection). Results: PF increased the content of DOPAC and NE in the cortex, and increased the content of NE and decreased the content of 5-HT in the medulla of the homogenized mice brain tissue. By microdialysis, PF increased the content of DOPAC and 5-HIAA in anesthetic rat cortex and expanded the content of DOPAC, HVA, and 5-HIAA in anesthetic rat striatum. Conclusions: It reveals that PF could activate the release of monoamines and increase their metabolites in the rodent brain. EDP Sciences 2019-05-24 /pmc/articles/PMC6538943/ /pubmed/31124457 http://dx.doi.org/10.1051/bmdcn/2019090211 Text en © Author(s) 2019. This article is published with open access by China Medical University Open Access This article is distributed under terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) which permits any use, distribution, and reproduction in any medium, provided original author(s) and source are credited. |
spellingShingle | Original Article Lin, Yuh-Tzy Huang, Wei-Shih Tsai, Huei-Yann Lee, Min-Min Chen, Yuh-Fung In vivo microdialysis and in vitro HPLC analysis of the impact of paeoniflorin on the monoamine levels and their metabolites in the rodent brain |
title | In vivo microdialysis and in vitro HPLC analysis of the impact of paeoniflorin on the monoamine levels and their metabolites in the rodent brain |
title_full | In vivo microdialysis and in vitro HPLC analysis of the impact of paeoniflorin on the monoamine levels and their metabolites in the rodent brain |
title_fullStr | In vivo microdialysis and in vitro HPLC analysis of the impact of paeoniflorin on the monoamine levels and their metabolites in the rodent brain |
title_full_unstemmed | In vivo microdialysis and in vitro HPLC analysis of the impact of paeoniflorin on the monoamine levels and their metabolites in the rodent brain |
title_short | In vivo microdialysis and in vitro HPLC analysis of the impact of paeoniflorin on the monoamine levels and their metabolites in the rodent brain |
title_sort | in vivo microdialysis and in vitro hplc analysis of the impact of paeoniflorin on the monoamine levels and their metabolites in the rodent brain |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6538943/ https://www.ncbi.nlm.nih.gov/pubmed/31124457 http://dx.doi.org/10.1051/bmdcn/2019090211 |
work_keys_str_mv | AT linyuhtzy invivomicrodialysisandinvitrohplcanalysisoftheimpactofpaeoniflorinonthemonoaminelevelsandtheirmetabolitesintherodentbrain AT huangweishih invivomicrodialysisandinvitrohplcanalysisoftheimpactofpaeoniflorinonthemonoaminelevelsandtheirmetabolitesintherodentbrain AT tsaihueiyann invivomicrodialysisandinvitrohplcanalysisoftheimpactofpaeoniflorinonthemonoaminelevelsandtheirmetabolitesintherodentbrain AT leeminmin invivomicrodialysisandinvitrohplcanalysisoftheimpactofpaeoniflorinonthemonoaminelevelsandtheirmetabolitesintherodentbrain AT chenyuhfung invivomicrodialysisandinvitrohplcanalysisoftheimpactofpaeoniflorinonthemonoaminelevelsandtheirmetabolitesintherodentbrain |