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Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence—A Transcriptomic Study

This paper aims to identify and describe new genetic markers involved in the processes of protein expression and modification reflected in the change of mitochondrial activity before and after in vitro maturation of the oocyte. Porcine oocytes collected from the ovaries of slaughtered landrace gilts...

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Autores principales: Chermuła, Błażej, Brązert, Maciej, Jeseta, Michal, Ożegowska, Katarzyna, Kocherova, Ievgenia, Jankowski, Maurycy, Celichowski, Piotr, Sujka-Kordowska, Patrycja, Konwerska, Aneta, Piotrowska-Kempisty, Hanna, Budna-Tukan, Joanna, Antosik, Paweł, Bukowska, Dorota, Machatkova, Marie, Brussow, Klaus P., Skowroński, Mariusz T., Pawelczyk, Leszek, Bruska, Małgorzata, Nowicki, Michał, Kempisty, Bartosz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539048/
https://www.ncbi.nlm.nih.gov/pubmed/31067669
http://dx.doi.org/10.3390/ijms20092238
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author Chermuła, Błażej
Brązert, Maciej
Jeseta, Michal
Ożegowska, Katarzyna
Kocherova, Ievgenia
Jankowski, Maurycy
Celichowski, Piotr
Sujka-Kordowska, Patrycja
Konwerska, Aneta
Piotrowska-Kempisty, Hanna
Budna-Tukan, Joanna
Antosik, Paweł
Bukowska, Dorota
Machatkova, Marie
Brussow, Klaus P.
Skowroński, Mariusz T.
Pawelczyk, Leszek
Bruska, Małgorzata
Nowicki, Michał
Kempisty, Bartosz
author_facet Chermuła, Błażej
Brązert, Maciej
Jeseta, Michal
Ożegowska, Katarzyna
Kocherova, Ievgenia
Jankowski, Maurycy
Celichowski, Piotr
Sujka-Kordowska, Patrycja
Konwerska, Aneta
Piotrowska-Kempisty, Hanna
Budna-Tukan, Joanna
Antosik, Paweł
Bukowska, Dorota
Machatkova, Marie
Brussow, Klaus P.
Skowroński, Mariusz T.
Pawelczyk, Leszek
Bruska, Małgorzata
Nowicki, Michał
Kempisty, Bartosz
author_sort Chermuła, Błażej
collection PubMed
description This paper aims to identify and describe new genetic markers involved in the processes of protein expression and modification reflected in the change of mitochondrial activity before and after in vitro maturation of the oocyte. Porcine oocytes collected from the ovaries of slaughtered landrace gilts were subjected to the process of in vitro maturation. Transcriptomic changes in the expression profile of oocyte genes involved in response to hypoxia, the transmembrane protein receptor serine threonine kinase signaling pathway, the “transforming growth factor β receptor signaling pathway”, “response to protein stimulus”, and “response to organic substance” were investigated using microarrays. The expression values of these genes in oocytes was analyzed before (immature) and after (mature) in vitro maturation, with significant differences found. All the significantly altered genes showed downregulation after the maturation process. The most changed genes from these gene ontologies, FOS, ID2, VEGFA, BTG2, CYR61, ESR1, AR, TACR3, CCND2, CHRDL1, were chosen to be further validated, described and related to the literature. Additionally, the mitochondrial activity of the analyzed oocytes was measured using specific dyes. We found that the mitochondrial activity was higher before the maturation process. The analysis of these results and the available literature provides a novel insight on the processes that occur during in vitro oocyte maturation. While this knowledge may prove to be useful in further research of the procedures commonly associated with in vitro fertilization procedures, it serves mostly as a basic reference for further proteomic, in vivo, and clinical studies that are necessary to translate it into practical applications.
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spelling pubmed-65390482019-06-04 Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence—A Transcriptomic Study Chermuła, Błażej Brązert, Maciej Jeseta, Michal Ożegowska, Katarzyna Kocherova, Ievgenia Jankowski, Maurycy Celichowski, Piotr Sujka-Kordowska, Patrycja Konwerska, Aneta Piotrowska-Kempisty, Hanna Budna-Tukan, Joanna Antosik, Paweł Bukowska, Dorota Machatkova, Marie Brussow, Klaus P. Skowroński, Mariusz T. Pawelczyk, Leszek Bruska, Małgorzata Nowicki, Michał Kempisty, Bartosz Int J Mol Sci Article This paper aims to identify and describe new genetic markers involved in the processes of protein expression and modification reflected in the change of mitochondrial activity before and after in vitro maturation of the oocyte. Porcine oocytes collected from the ovaries of slaughtered landrace gilts were subjected to the process of in vitro maturation. Transcriptomic changes in the expression profile of oocyte genes involved in response to hypoxia, the transmembrane protein receptor serine threonine kinase signaling pathway, the “transforming growth factor β receptor signaling pathway”, “response to protein stimulus”, and “response to organic substance” were investigated using microarrays. The expression values of these genes in oocytes was analyzed before (immature) and after (mature) in vitro maturation, with significant differences found. All the significantly altered genes showed downregulation after the maturation process. The most changed genes from these gene ontologies, FOS, ID2, VEGFA, BTG2, CYR61, ESR1, AR, TACR3, CCND2, CHRDL1, were chosen to be further validated, described and related to the literature. Additionally, the mitochondrial activity of the analyzed oocytes was measured using specific dyes. We found that the mitochondrial activity was higher before the maturation process. The analysis of these results and the available literature provides a novel insight on the processes that occur during in vitro oocyte maturation. While this knowledge may prove to be useful in further research of the procedures commonly associated with in vitro fertilization procedures, it serves mostly as a basic reference for further proteomic, in vivo, and clinical studies that are necessary to translate it into practical applications. MDPI 2019-05-07 /pmc/articles/PMC6539048/ /pubmed/31067669 http://dx.doi.org/10.3390/ijms20092238 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chermuła, Błażej
Brązert, Maciej
Jeseta, Michal
Ożegowska, Katarzyna
Kocherova, Ievgenia
Jankowski, Maurycy
Celichowski, Piotr
Sujka-Kordowska, Patrycja
Konwerska, Aneta
Piotrowska-Kempisty, Hanna
Budna-Tukan, Joanna
Antosik, Paweł
Bukowska, Dorota
Machatkova, Marie
Brussow, Klaus P.
Skowroński, Mariusz T.
Pawelczyk, Leszek
Bruska, Małgorzata
Nowicki, Michał
Kempisty, Bartosz
Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence—A Transcriptomic Study
title Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence—A Transcriptomic Study
title_full Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence—A Transcriptomic Study
title_fullStr Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence—A Transcriptomic Study
title_full_unstemmed Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence—A Transcriptomic Study
title_short Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence—A Transcriptomic Study
title_sort transcriptomic pattern of genes regulating protein response and status of mitochondrial activity are related to oocyte maturational competence—a transcriptomic study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539048/
https://www.ncbi.nlm.nih.gov/pubmed/31067669
http://dx.doi.org/10.3390/ijms20092238
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