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A Viral Polymerase Inhibitor Reduces Zika Virus Replication in the Reproductive Organs of Male Mice

In humans, Zika virus and viral RNA have been detected in semen up to 2.2 months and 6 months post infection (pi), respectively. Although the contribution of sexual transmission to the spread of ZIKV is too low to sustain an outbreak, it can increase the risk of infection and the epidemic size as we...

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Autores principales: Jacobs, Sofie, Delang, Leen, Verbeken, Eric, Neyts, Johan, Kaptein, Suzanne J.F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539190/
https://www.ncbi.nlm.nih.gov/pubmed/31032814
http://dx.doi.org/10.3390/ijms20092122
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author Jacobs, Sofie
Delang, Leen
Verbeken, Eric
Neyts, Johan
Kaptein, Suzanne J.F.
author_facet Jacobs, Sofie
Delang, Leen
Verbeken, Eric
Neyts, Johan
Kaptein, Suzanne J.F.
author_sort Jacobs, Sofie
collection PubMed
description In humans, Zika virus and viral RNA have been detected in semen up to 2.2 months and 6 months post infection (pi), respectively. Although the contribution of sexual transmission to the spread of ZIKV is too low to sustain an outbreak, it can increase the risk of infection and the epidemic size as well as prolong the duration of an outbreak. In this study, we explored the potential of antivirals to serve as an effective strategy to prevent sexual transmission. Male AG129 mice infected with a ZIKV isolate from Suriname were treated with the nucleoside analog, 7-deaza-2′-C-methyladenosine (7DMA), that was previously shown to be efficacious in reducing ZIKV viremia and delaying ZIKV-induced disease in mice. Following treatment, viral RNA and infectious virus titers were consistently reduced in the male reproductive organs compared to vehicle-treated mice. This reduction of ZIKV loads in the testis was confirmed by the detection of lower levels of ZIKV antigens. Our data illustrate the value of this mouse model to validate the efficacy of new potential ZIKV drugs at the level of the male reproductive system.
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spelling pubmed-65391902019-06-04 A Viral Polymerase Inhibitor Reduces Zika Virus Replication in the Reproductive Organs of Male Mice Jacobs, Sofie Delang, Leen Verbeken, Eric Neyts, Johan Kaptein, Suzanne J.F. Int J Mol Sci Communication In humans, Zika virus and viral RNA have been detected in semen up to 2.2 months and 6 months post infection (pi), respectively. Although the contribution of sexual transmission to the spread of ZIKV is too low to sustain an outbreak, it can increase the risk of infection and the epidemic size as well as prolong the duration of an outbreak. In this study, we explored the potential of antivirals to serve as an effective strategy to prevent sexual transmission. Male AG129 mice infected with a ZIKV isolate from Suriname were treated with the nucleoside analog, 7-deaza-2′-C-methyladenosine (7DMA), that was previously shown to be efficacious in reducing ZIKV viremia and delaying ZIKV-induced disease in mice. Following treatment, viral RNA and infectious virus titers were consistently reduced in the male reproductive organs compared to vehicle-treated mice. This reduction of ZIKV loads in the testis was confirmed by the detection of lower levels of ZIKV antigens. Our data illustrate the value of this mouse model to validate the efficacy of new potential ZIKV drugs at the level of the male reproductive system. MDPI 2019-04-29 /pmc/articles/PMC6539190/ /pubmed/31032814 http://dx.doi.org/10.3390/ijms20092122 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Jacobs, Sofie
Delang, Leen
Verbeken, Eric
Neyts, Johan
Kaptein, Suzanne J.F.
A Viral Polymerase Inhibitor Reduces Zika Virus Replication in the Reproductive Organs of Male Mice
title A Viral Polymerase Inhibitor Reduces Zika Virus Replication in the Reproductive Organs of Male Mice
title_full A Viral Polymerase Inhibitor Reduces Zika Virus Replication in the Reproductive Organs of Male Mice
title_fullStr A Viral Polymerase Inhibitor Reduces Zika Virus Replication in the Reproductive Organs of Male Mice
title_full_unstemmed A Viral Polymerase Inhibitor Reduces Zika Virus Replication in the Reproductive Organs of Male Mice
title_short A Viral Polymerase Inhibitor Reduces Zika Virus Replication in the Reproductive Organs of Male Mice
title_sort viral polymerase inhibitor reduces zika virus replication in the reproductive organs of male mice
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539190/
https://www.ncbi.nlm.nih.gov/pubmed/31032814
http://dx.doi.org/10.3390/ijms20092122
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