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Bisphenol A Activates Calcium Influx in Immortalized GnRH Neurons

Bisphenol A (BPA) is one of the most widely used chemicals worldwide, e.g., as a component of plastic containers for food and water. It is considered to exert an estrogenic effect, by mimicking estradiol (E2) action. Because of this widespread presence, it has attracted the interest and concern of r...

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Autores principales: Ruffinatti, Federico Alessandro, Gilardino, Alessandra, Secchi, Valter, Cottone, Erika, Lovisolo, Davide, Bovolin, Patrizia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539360/
https://www.ncbi.nlm.nih.gov/pubmed/31052388
http://dx.doi.org/10.3390/ijms20092160
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author Ruffinatti, Federico Alessandro
Gilardino, Alessandra
Secchi, Valter
Cottone, Erika
Lovisolo, Davide
Bovolin, Patrizia
author_facet Ruffinatti, Federico Alessandro
Gilardino, Alessandra
Secchi, Valter
Cottone, Erika
Lovisolo, Davide
Bovolin, Patrizia
author_sort Ruffinatti, Federico Alessandro
collection PubMed
description Bisphenol A (BPA) is one of the most widely used chemicals worldwide, e.g., as a component of plastic containers for food and water. It is considered to exert an estrogenic effect, by mimicking estradiol (E2) action. Because of this widespread presence, it has attracted the interest and concern of researchers and regulators. Despite the vast amount of related literature, the potential adverse effects of environmentally significant doses of BPA are still object of controversy, and the mechanisms by which it can perturb endocrine functions, and particularly the neuroendocrine axis, are not adequately understood. One of the ways by which endocrine disruptors (EDCs) can exert their effects is the perturbation of calcium signaling mechanisms. In this study, we addressed the issue of the impact of BPA on the neuroendocrine system with an in vitro approach, using a consolidated model of immortalized Gonadotropin-Releasing Hormone (GnRH) expressing neurons, the GT1–7 cell line, focusing on the calcium signals activated by the endocrine disruptor. The investigation was limited to biologically relevant doses (nM–µM range). We found that BPA induced moderate increases in intracellular calcium concentration, comparable with those induced by nanomolar doses of E2, without affecting cell survival and with only a minor effect on proliferation.
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spelling pubmed-65393602019-06-04 Bisphenol A Activates Calcium Influx in Immortalized GnRH Neurons Ruffinatti, Federico Alessandro Gilardino, Alessandra Secchi, Valter Cottone, Erika Lovisolo, Davide Bovolin, Patrizia Int J Mol Sci Article Bisphenol A (BPA) is one of the most widely used chemicals worldwide, e.g., as a component of plastic containers for food and water. It is considered to exert an estrogenic effect, by mimicking estradiol (E2) action. Because of this widespread presence, it has attracted the interest and concern of researchers and regulators. Despite the vast amount of related literature, the potential adverse effects of environmentally significant doses of BPA are still object of controversy, and the mechanisms by which it can perturb endocrine functions, and particularly the neuroendocrine axis, are not adequately understood. One of the ways by which endocrine disruptors (EDCs) can exert their effects is the perturbation of calcium signaling mechanisms. In this study, we addressed the issue of the impact of BPA on the neuroendocrine system with an in vitro approach, using a consolidated model of immortalized Gonadotropin-Releasing Hormone (GnRH) expressing neurons, the GT1–7 cell line, focusing on the calcium signals activated by the endocrine disruptor. The investigation was limited to biologically relevant doses (nM–µM range). We found that BPA induced moderate increases in intracellular calcium concentration, comparable with those induced by nanomolar doses of E2, without affecting cell survival and with only a minor effect on proliferation. MDPI 2019-05-01 /pmc/articles/PMC6539360/ /pubmed/31052388 http://dx.doi.org/10.3390/ijms20092160 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ruffinatti, Federico Alessandro
Gilardino, Alessandra
Secchi, Valter
Cottone, Erika
Lovisolo, Davide
Bovolin, Patrizia
Bisphenol A Activates Calcium Influx in Immortalized GnRH Neurons
title Bisphenol A Activates Calcium Influx in Immortalized GnRH Neurons
title_full Bisphenol A Activates Calcium Influx in Immortalized GnRH Neurons
title_fullStr Bisphenol A Activates Calcium Influx in Immortalized GnRH Neurons
title_full_unstemmed Bisphenol A Activates Calcium Influx in Immortalized GnRH Neurons
title_short Bisphenol A Activates Calcium Influx in Immortalized GnRH Neurons
title_sort bisphenol a activates calcium influx in immortalized gnrh neurons
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539360/
https://www.ncbi.nlm.nih.gov/pubmed/31052388
http://dx.doi.org/10.3390/ijms20092160
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