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Structure/Activity Analysis of TASK-3 Channel Antagonists Based on a 5,6,7,8 tetrahydropyrido[4,3-d]pyrimidine
TASK-3 potassium (K(+)) channels are highly expressed in the central nervous system, regulating the membrane potential of excitable cells. TASK-3 is involved in neurotransmitter action and has been identified as an oncogenic K(+) channel. For this reason, the understanding of the action mechanism of...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539479/ https://www.ncbi.nlm.nih.gov/pubmed/31067753 http://dx.doi.org/10.3390/ijms20092252 |
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author | Ramírez, David Bedoya, Mauricio Kiper, Aytug K. Rinné, Susanne Morales-Navarro, Samuel Hernández-Rodríguez, Erix W. Sepúlveda, Francisco V. Decher, Niels González, Wendy |
author_facet | Ramírez, David Bedoya, Mauricio Kiper, Aytug K. Rinné, Susanne Morales-Navarro, Samuel Hernández-Rodríguez, Erix W. Sepúlveda, Francisco V. Decher, Niels González, Wendy |
author_sort | Ramírez, David |
collection | PubMed |
description | TASK-3 potassium (K(+)) channels are highly expressed in the central nervous system, regulating the membrane potential of excitable cells. TASK-3 is involved in neurotransmitter action and has been identified as an oncogenic K(+) channel. For this reason, the understanding of the action mechanism of pharmacological modulators of these channels is essential to obtain new therapeutic strategies. In this study we describe the binding mode of the potent antagonist PK-THPP into the TASK-3 channel. PK-THPP blocks TASK-1, the closest relative channel of TASK-3, with almost nine-times less potency. Our results confirm that the binding is influenced by the fenestrations state of TASK-3 channels and occurs when they are open. The binding is mainly governed by hydrophobic contacts between the blocker and the residues of the binding site. These interactions occur not only for PK-THPP, but also for the antagonist series based on 5,6,7,8 tetrahydropyrido[4,3-d]pyrimidine scaffold (THPP series). However, the marked difference in the potency of THPP series compounds such as 20b, 21, 22 and 23 (PK-THPP) respect to compounds such as 17b, inhibiting TASK-3 channels in the micromolar range is due to the presence of a hydrogen bond acceptor group that can establish interactions with the threonines of the selectivity filter. |
format | Online Article Text |
id | pubmed-6539479 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65394792019-06-04 Structure/Activity Analysis of TASK-3 Channel Antagonists Based on a 5,6,7,8 tetrahydropyrido[4,3-d]pyrimidine Ramírez, David Bedoya, Mauricio Kiper, Aytug K. Rinné, Susanne Morales-Navarro, Samuel Hernández-Rodríguez, Erix W. Sepúlveda, Francisco V. Decher, Niels González, Wendy Int J Mol Sci Article TASK-3 potassium (K(+)) channels are highly expressed in the central nervous system, regulating the membrane potential of excitable cells. TASK-3 is involved in neurotransmitter action and has been identified as an oncogenic K(+) channel. For this reason, the understanding of the action mechanism of pharmacological modulators of these channels is essential to obtain new therapeutic strategies. In this study we describe the binding mode of the potent antagonist PK-THPP into the TASK-3 channel. PK-THPP blocks TASK-1, the closest relative channel of TASK-3, with almost nine-times less potency. Our results confirm that the binding is influenced by the fenestrations state of TASK-3 channels and occurs when they are open. The binding is mainly governed by hydrophobic contacts between the blocker and the residues of the binding site. These interactions occur not only for PK-THPP, but also for the antagonist series based on 5,6,7,8 tetrahydropyrido[4,3-d]pyrimidine scaffold (THPP series). However, the marked difference in the potency of THPP series compounds such as 20b, 21, 22 and 23 (PK-THPP) respect to compounds such as 17b, inhibiting TASK-3 channels in the micromolar range is due to the presence of a hydrogen bond acceptor group that can establish interactions with the threonines of the selectivity filter. MDPI 2019-05-07 /pmc/articles/PMC6539479/ /pubmed/31067753 http://dx.doi.org/10.3390/ijms20092252 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ramírez, David Bedoya, Mauricio Kiper, Aytug K. Rinné, Susanne Morales-Navarro, Samuel Hernández-Rodríguez, Erix W. Sepúlveda, Francisco V. Decher, Niels González, Wendy Structure/Activity Analysis of TASK-3 Channel Antagonists Based on a 5,6,7,8 tetrahydropyrido[4,3-d]pyrimidine |
title | Structure/Activity Analysis of TASK-3 Channel Antagonists Based on a 5,6,7,8 tetrahydropyrido[4,3-d]pyrimidine |
title_full | Structure/Activity Analysis of TASK-3 Channel Antagonists Based on a 5,6,7,8 tetrahydropyrido[4,3-d]pyrimidine |
title_fullStr | Structure/Activity Analysis of TASK-3 Channel Antagonists Based on a 5,6,7,8 tetrahydropyrido[4,3-d]pyrimidine |
title_full_unstemmed | Structure/Activity Analysis of TASK-3 Channel Antagonists Based on a 5,6,7,8 tetrahydropyrido[4,3-d]pyrimidine |
title_short | Structure/Activity Analysis of TASK-3 Channel Antagonists Based on a 5,6,7,8 tetrahydropyrido[4,3-d]pyrimidine |
title_sort | structure/activity analysis of task-3 channel antagonists based on a 5,6,7,8 tetrahydropyrido[4,3-d]pyrimidine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539479/ https://www.ncbi.nlm.nih.gov/pubmed/31067753 http://dx.doi.org/10.3390/ijms20092252 |
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