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Antioxidant and Photoprotective Activity of Apigenin and Its Potassium Salt Derivative in Human Keratinocytes and Absorption in Caco-2 Cell Monolayers

Ultraviolet (UV) radiation, especially types A (UVA) and B (UVB), is one of the main causes of skin disorders, including photoaging and skin cancer. Ultraviolent radiation causes oxidative stress, inflammation, p53 induction, DNA damage, mutagenesis, and oxidation of various molecules such as lipids...

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Autores principales: Sánchez-Marzo, Noelia, Pérez-Sánchez, Almudena, Ruiz-Torres, Verónica, Martínez-Tébar, Adrián, Castillo, Julián, Herranz-López, María, Barrajón-Catalán, Enrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539602/
https://www.ncbi.nlm.nih.gov/pubmed/31052292
http://dx.doi.org/10.3390/ijms20092148
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author Sánchez-Marzo, Noelia
Pérez-Sánchez, Almudena
Ruiz-Torres, Verónica
Martínez-Tébar, Adrián
Castillo, Julián
Herranz-López, María
Barrajón-Catalán, Enrique
author_facet Sánchez-Marzo, Noelia
Pérez-Sánchez, Almudena
Ruiz-Torres, Verónica
Martínez-Tébar, Adrián
Castillo, Julián
Herranz-López, María
Barrajón-Catalán, Enrique
author_sort Sánchez-Marzo, Noelia
collection PubMed
description Ultraviolet (UV) radiation, especially types A (UVA) and B (UVB), is one of the main causes of skin disorders, including photoaging and skin cancer. Ultraviolent radiation causes oxidative stress, inflammation, p53 induction, DNA damage, mutagenesis, and oxidation of various molecules such as lipids and proteins. In recent decades, the use of polyphenols as molecules with an antioxidant and anti-inflammatory capacity has increased. However, some of these compounds are poorly soluble, and information regarding their absorption and bioavailability is scarce. The main objective of this study was to compare the intestinal absorption and biological activity of apigenin and its more soluble potassium salt (apigenin-K) in terms of antioxidant and photoprotective capacity. Photoprotective effects against UVA and UVB radiation were studied in human keratinocytes, and antioxidant capacity was determined by different methods, including trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assays. Finally, the intestinal absorption of both apigenins was determined using an in vitro Caco-2 cell model. Apigenin showed a slightly higher antioxidant capacity in antioxidant activity assays when compared with apigenin-K. However, no significant differences were obtained for their photoprotective capacities against UVA or UVB. Results indicated that both apigenins protected cell viability in approximately 50% at 5 J/m(2) of UVA and 90% at 500 J/m(2) of UVB radiation. Regarding intestinal absorption, both apigenins showed similar apparent permeabilities (P(app)), 1.81 × 10(−5) cm/s and 1.78 × 10(−5) cm/s, respectively. Taken together, these results suggest that both apigenins may be interesting candidates for the development of oral (nutraceutical) and topical photoprotective ingredients against UVA and UVB-induced skin damage, but the increased water solubility of apigenin-K makes it the best candidate for further development.
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spelling pubmed-65396022019-06-04 Antioxidant and Photoprotective Activity of Apigenin and Its Potassium Salt Derivative in Human Keratinocytes and Absorption in Caco-2 Cell Monolayers Sánchez-Marzo, Noelia Pérez-Sánchez, Almudena Ruiz-Torres, Verónica Martínez-Tébar, Adrián Castillo, Julián Herranz-López, María Barrajón-Catalán, Enrique Int J Mol Sci Article Ultraviolet (UV) radiation, especially types A (UVA) and B (UVB), is one of the main causes of skin disorders, including photoaging and skin cancer. Ultraviolent radiation causes oxidative stress, inflammation, p53 induction, DNA damage, mutagenesis, and oxidation of various molecules such as lipids and proteins. In recent decades, the use of polyphenols as molecules with an antioxidant and anti-inflammatory capacity has increased. However, some of these compounds are poorly soluble, and information regarding their absorption and bioavailability is scarce. The main objective of this study was to compare the intestinal absorption and biological activity of apigenin and its more soluble potassium salt (apigenin-K) in terms of antioxidant and photoprotective capacity. Photoprotective effects against UVA and UVB radiation were studied in human keratinocytes, and antioxidant capacity was determined by different methods, including trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assays. Finally, the intestinal absorption of both apigenins was determined using an in vitro Caco-2 cell model. Apigenin showed a slightly higher antioxidant capacity in antioxidant activity assays when compared with apigenin-K. However, no significant differences were obtained for their photoprotective capacities against UVA or UVB. Results indicated that both apigenins protected cell viability in approximately 50% at 5 J/m(2) of UVA and 90% at 500 J/m(2) of UVB radiation. Regarding intestinal absorption, both apigenins showed similar apparent permeabilities (P(app)), 1.81 × 10(−5) cm/s and 1.78 × 10(−5) cm/s, respectively. Taken together, these results suggest that both apigenins may be interesting candidates for the development of oral (nutraceutical) and topical photoprotective ingredients against UVA and UVB-induced skin damage, but the increased water solubility of apigenin-K makes it the best candidate for further development. MDPI 2019-04-30 /pmc/articles/PMC6539602/ /pubmed/31052292 http://dx.doi.org/10.3390/ijms20092148 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sánchez-Marzo, Noelia
Pérez-Sánchez, Almudena
Ruiz-Torres, Verónica
Martínez-Tébar, Adrián
Castillo, Julián
Herranz-López, María
Barrajón-Catalán, Enrique
Antioxidant and Photoprotective Activity of Apigenin and Its Potassium Salt Derivative in Human Keratinocytes and Absorption in Caco-2 Cell Monolayers
title Antioxidant and Photoprotective Activity of Apigenin and Its Potassium Salt Derivative in Human Keratinocytes and Absorption in Caco-2 Cell Monolayers
title_full Antioxidant and Photoprotective Activity of Apigenin and Its Potassium Salt Derivative in Human Keratinocytes and Absorption in Caco-2 Cell Monolayers
title_fullStr Antioxidant and Photoprotective Activity of Apigenin and Its Potassium Salt Derivative in Human Keratinocytes and Absorption in Caco-2 Cell Monolayers
title_full_unstemmed Antioxidant and Photoprotective Activity of Apigenin and Its Potassium Salt Derivative in Human Keratinocytes and Absorption in Caco-2 Cell Monolayers
title_short Antioxidant and Photoprotective Activity of Apigenin and Its Potassium Salt Derivative in Human Keratinocytes and Absorption in Caco-2 Cell Monolayers
title_sort antioxidant and photoprotective activity of apigenin and its potassium salt derivative in human keratinocytes and absorption in caco-2 cell monolayers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539602/
https://www.ncbi.nlm.nih.gov/pubmed/31052292
http://dx.doi.org/10.3390/ijms20092148
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