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Polyphenols-Rich Fruit (Euterpe edulis Mart.) Prevents Peripheral Inflammatory Pathway Activation by the Short-Term High-Fat Diet

Juçara berry is a potential inflammatory modulator, rich in dietary fiber, fatty acids, and anthocyanins. Considering this, we evaluated the high-fat diet (HFD) intake supplemented with different doses of freeze-dried juçara pulp on the TLR4 pathway. Twenty-seven male Wistar rats with ad libitum acc...

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Autores principales: Santamarina, Aline Boveto, Jamar, Giovana, Mennitti, Laís Vales, Ribeiro, Daniel Araki, Cardoso, Caroline Margonato, de Rosso, Veridiana Vera, Oyama, Lila Missae, Pisani, Luciana Pellegrini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539628/
https://www.ncbi.nlm.nih.gov/pubmed/31035535
http://dx.doi.org/10.3390/molecules24091655
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author Santamarina, Aline Boveto
Jamar, Giovana
Mennitti, Laís Vales
Ribeiro, Daniel Araki
Cardoso, Caroline Margonato
de Rosso, Veridiana Vera
Oyama, Lila Missae
Pisani, Luciana Pellegrini
author_facet Santamarina, Aline Boveto
Jamar, Giovana
Mennitti, Laís Vales
Ribeiro, Daniel Araki
Cardoso, Caroline Margonato
de Rosso, Veridiana Vera
Oyama, Lila Missae
Pisani, Luciana Pellegrini
author_sort Santamarina, Aline Boveto
collection PubMed
description Juçara berry is a potential inflammatory modulator, rich in dietary fiber, fatty acids, and anthocyanins. Considering this, we evaluated the high-fat diet (HFD) intake supplemented with different doses of freeze-dried juçara pulp on the TLR4 pathway. Twenty-seven male Wistar rats with ad libitum access to food and water were divided into four experimental groups: control standard chow group (C); high-fat diet control group (HFC); high-fat diet juçara 0.25% group (HFJ0.25%); and high-fat diet juçara 0.5% group (HFJ0.5%). The inflammatory parameters were analyzed by ELISA and Western blotting in liver and retroperitoneal adipose tissue (RET). The HFJ0.25% group had the energy intake, aspartate transaminase (AST) levels, and liver triacylglycerol accumulation reduced; also, the tumor necrosis factor α (TNF-α) and TNF receptor-associated factor 6 (TRAF6) expression in RET were reduced. However, there were no changes in other protein expressions in liver and adipose tissue. Adiposity and pNFκBp50 had a positive correlation in HFC and HFJ0.5%, but not in the C group and HFJ0.25%. The necrosis hepatic score did not change with treatment; however, the serum (AST) levels and the hepatic triacylglycerol were increased in HFC and HFJ0.5%. These results demonstrated that one week of HFD intake triggered pro-inflammatory mechanisms and liver injury. Additionally, 0.25% juçara prevented inflammatory pathway activation, body weight gain, and liver damage
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spelling pubmed-65396282019-05-31 Polyphenols-Rich Fruit (Euterpe edulis Mart.) Prevents Peripheral Inflammatory Pathway Activation by the Short-Term High-Fat Diet Santamarina, Aline Boveto Jamar, Giovana Mennitti, Laís Vales Ribeiro, Daniel Araki Cardoso, Caroline Margonato de Rosso, Veridiana Vera Oyama, Lila Missae Pisani, Luciana Pellegrini Molecules Article Juçara berry is a potential inflammatory modulator, rich in dietary fiber, fatty acids, and anthocyanins. Considering this, we evaluated the high-fat diet (HFD) intake supplemented with different doses of freeze-dried juçara pulp on the TLR4 pathway. Twenty-seven male Wistar rats with ad libitum access to food and water were divided into four experimental groups: control standard chow group (C); high-fat diet control group (HFC); high-fat diet juçara 0.25% group (HFJ0.25%); and high-fat diet juçara 0.5% group (HFJ0.5%). The inflammatory parameters were analyzed by ELISA and Western blotting in liver and retroperitoneal adipose tissue (RET). The HFJ0.25% group had the energy intake, aspartate transaminase (AST) levels, and liver triacylglycerol accumulation reduced; also, the tumor necrosis factor α (TNF-α) and TNF receptor-associated factor 6 (TRAF6) expression in RET were reduced. However, there were no changes in other protein expressions in liver and adipose tissue. Adiposity and pNFκBp50 had a positive correlation in HFC and HFJ0.5%, but not in the C group and HFJ0.25%. The necrosis hepatic score did not change with treatment; however, the serum (AST) levels and the hepatic triacylglycerol were increased in HFC and HFJ0.5%. These results demonstrated that one week of HFD intake triggered pro-inflammatory mechanisms and liver injury. Additionally, 0.25% juçara prevented inflammatory pathway activation, body weight gain, and liver damage MDPI 2019-04-27 /pmc/articles/PMC6539628/ /pubmed/31035535 http://dx.doi.org/10.3390/molecules24091655 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Santamarina, Aline Boveto
Jamar, Giovana
Mennitti, Laís Vales
Ribeiro, Daniel Araki
Cardoso, Caroline Margonato
de Rosso, Veridiana Vera
Oyama, Lila Missae
Pisani, Luciana Pellegrini
Polyphenols-Rich Fruit (Euterpe edulis Mart.) Prevents Peripheral Inflammatory Pathway Activation by the Short-Term High-Fat Diet
title Polyphenols-Rich Fruit (Euterpe edulis Mart.) Prevents Peripheral Inflammatory Pathway Activation by the Short-Term High-Fat Diet
title_full Polyphenols-Rich Fruit (Euterpe edulis Mart.) Prevents Peripheral Inflammatory Pathway Activation by the Short-Term High-Fat Diet
title_fullStr Polyphenols-Rich Fruit (Euterpe edulis Mart.) Prevents Peripheral Inflammatory Pathway Activation by the Short-Term High-Fat Diet
title_full_unstemmed Polyphenols-Rich Fruit (Euterpe edulis Mart.) Prevents Peripheral Inflammatory Pathway Activation by the Short-Term High-Fat Diet
title_short Polyphenols-Rich Fruit (Euterpe edulis Mart.) Prevents Peripheral Inflammatory Pathway Activation by the Short-Term High-Fat Diet
title_sort polyphenols-rich fruit (euterpe edulis mart.) prevents peripheral inflammatory pathway activation by the short-term high-fat diet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539628/
https://www.ncbi.nlm.nih.gov/pubmed/31035535
http://dx.doi.org/10.3390/molecules24091655
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