Protein-Based Fingerprint Analysis for the Identification of Ranae Oviductus Using RP-HPLC

This work demonstrated a method combining reversed-phase high-performance liquid chromatography (RP-HPLC) with chemometrics analysis to identify the authenticity of Ranae Oviductus. The fingerprint chromatograms of the Ranae Oviductus protein were established through an Agilent Zorbax 300SB-C8 colum...

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Detalles Bibliográficos
Autores principales: Gan, Yuanshuai, Xiao, Yao, Wang, Shihan, Guo, Hongye, Liu, Min, Wang, Zhihan, Wang, Yongsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539769/
https://www.ncbi.nlm.nih.gov/pubmed/31052194
http://dx.doi.org/10.3390/molecules24091687
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author Gan, Yuanshuai
Xiao, Yao
Wang, Shihan
Guo, Hongye
Liu, Min
Wang, Zhihan
Wang, Yongsheng
author_facet Gan, Yuanshuai
Xiao, Yao
Wang, Shihan
Guo, Hongye
Liu, Min
Wang, Zhihan
Wang, Yongsheng
author_sort Gan, Yuanshuai
collection PubMed
description This work demonstrated a method combining reversed-phase high-performance liquid chromatography (RP-HPLC) with chemometrics analysis to identify the authenticity of Ranae Oviductus. The fingerprint chromatograms of the Ranae Oviductus protein were established through an Agilent Zorbax 300SB-C8 column and diode array detection at 215 nm, using 0.085% TFA (v/v) in acetonitrile (A) and 0.1% TFA in ultrapure water (B) as mobile phase. The similarity was in the range of 0.779–0.980. The fingerprint chromatogram of Ranae Oviductus showed a significant difference with counterfeit products. Hierarchical clustering analysis (HCA) and principal component analysis (PCA) successfully identified Ranae Oviductus from the samples. These results indicated that the method established in this work was reliable.
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spelling pubmed-65397692019-05-31 Protein-Based Fingerprint Analysis for the Identification of Ranae Oviductus Using RP-HPLC Gan, Yuanshuai Xiao, Yao Wang, Shihan Guo, Hongye Liu, Min Wang, Zhihan Wang, Yongsheng Molecules Article This work demonstrated a method combining reversed-phase high-performance liquid chromatography (RP-HPLC) with chemometrics analysis to identify the authenticity of Ranae Oviductus. The fingerprint chromatograms of the Ranae Oviductus protein were established through an Agilent Zorbax 300SB-C8 column and diode array detection at 215 nm, using 0.085% TFA (v/v) in acetonitrile (A) and 0.1% TFA in ultrapure water (B) as mobile phase. The similarity was in the range of 0.779–0.980. The fingerprint chromatogram of Ranae Oviductus showed a significant difference with counterfeit products. Hierarchical clustering analysis (HCA) and principal component analysis (PCA) successfully identified Ranae Oviductus from the samples. These results indicated that the method established in this work was reliable. MDPI 2019-04-30 /pmc/articles/PMC6539769/ /pubmed/31052194 http://dx.doi.org/10.3390/molecules24091687 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gan, Yuanshuai
Xiao, Yao
Wang, Shihan
Guo, Hongye
Liu, Min
Wang, Zhihan
Wang, Yongsheng
Protein-Based Fingerprint Analysis for the Identification of Ranae Oviductus Using RP-HPLC
title Protein-Based Fingerprint Analysis for the Identification of Ranae Oviductus Using RP-HPLC
title_full Protein-Based Fingerprint Analysis for the Identification of Ranae Oviductus Using RP-HPLC
title_fullStr Protein-Based Fingerprint Analysis for the Identification of Ranae Oviductus Using RP-HPLC
title_full_unstemmed Protein-Based Fingerprint Analysis for the Identification of Ranae Oviductus Using RP-HPLC
title_short Protein-Based Fingerprint Analysis for the Identification of Ranae Oviductus Using RP-HPLC
title_sort protein-based fingerprint analysis for the identification of ranae oviductus using rp-hplc
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539769/
https://www.ncbi.nlm.nih.gov/pubmed/31052194
http://dx.doi.org/10.3390/molecules24091687
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