Cargando…
The Relationship between the HLA-G Polymorphism and sHLA-G Levels in Parental Pairs with High-Risk Pregnancy
Human leukocyte antigen G (HLA-G) is observed in immune system cells and other organs. It is a class Ib molecule, which plays a pivotal role in the implantation and maintenance of pregnancy. The aim of this study was to assess the relationship between serum sHLA-G levels and the HLA-G allele in pare...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539819/ https://www.ncbi.nlm.nih.gov/pubmed/31052398 http://dx.doi.org/10.3390/ijerph16091546 |
_version_ | 1783422479166865408 |
---|---|
author | Sipak, Olimpia Rył, Aleksandra Grzywacz, Anna Laszczyńska, Maria Zimny, Małgorzata Karakiewicz, Beata Rotter, Iwona Kosik-Bogacka, Danuta Cybulski, Cezary |
author_facet | Sipak, Olimpia Rył, Aleksandra Grzywacz, Anna Laszczyńska, Maria Zimny, Małgorzata Karakiewicz, Beata Rotter, Iwona Kosik-Bogacka, Danuta Cybulski, Cezary |
author_sort | Sipak, Olimpia |
collection | PubMed |
description | Human leukocyte antigen G (HLA-G) is observed in immune system cells and other organs. It is a class Ib molecule, which plays a pivotal role in the implantation and maintenance of pregnancy. The aim of this study was to assess the relationship between serum sHLA-G levels and the HLA-G allele in parental pairs with complicated obstetric histories. The clinical material consisted of 210 women and 190 men with the experience of a complicated or an unsuccessful pregnancy. The control group included parents―89 women and 86 men―lacking complicated obstetric histories. We applied genetic analysis methods: isolation of genomic DNA, sequencing, and determination of serum sHLA-G levels. There were no statistically significant differences in the frequencies of the HLA-G −725 C>G polymorphism between particular experimental groups compared with the control group (p > 0.05). The median sHLA-G levels in the women with the HLA-G10101 allele (15.4 U/mL) were significantly higher than in the women with other alleles (p < 0.05). The HLA-G 10101 allele seems to protect against antiphospholipid syndrome, which may be associated with increased serum sHLA-G levels in its carriers. The relationship between serum sHLA-G levels and the HLA-G polymorphisms in the Polish population requires further investigation. |
format | Online Article Text |
id | pubmed-6539819 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65398192019-06-05 The Relationship between the HLA-G Polymorphism and sHLA-G Levels in Parental Pairs with High-Risk Pregnancy Sipak, Olimpia Rył, Aleksandra Grzywacz, Anna Laszczyńska, Maria Zimny, Małgorzata Karakiewicz, Beata Rotter, Iwona Kosik-Bogacka, Danuta Cybulski, Cezary Int J Environ Res Public Health Article Human leukocyte antigen G (HLA-G) is observed in immune system cells and other organs. It is a class Ib molecule, which plays a pivotal role in the implantation and maintenance of pregnancy. The aim of this study was to assess the relationship between serum sHLA-G levels and the HLA-G allele in parental pairs with complicated obstetric histories. The clinical material consisted of 210 women and 190 men with the experience of a complicated or an unsuccessful pregnancy. The control group included parents―89 women and 86 men―lacking complicated obstetric histories. We applied genetic analysis methods: isolation of genomic DNA, sequencing, and determination of serum sHLA-G levels. There were no statistically significant differences in the frequencies of the HLA-G −725 C>G polymorphism between particular experimental groups compared with the control group (p > 0.05). The median sHLA-G levels in the women with the HLA-G10101 allele (15.4 U/mL) were significantly higher than in the women with other alleles (p < 0.05). The HLA-G 10101 allele seems to protect against antiphospholipid syndrome, which may be associated with increased serum sHLA-G levels in its carriers. The relationship between serum sHLA-G levels and the HLA-G polymorphisms in the Polish population requires further investigation. MDPI 2019-05-01 2019-05 /pmc/articles/PMC6539819/ /pubmed/31052398 http://dx.doi.org/10.3390/ijerph16091546 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sipak, Olimpia Rył, Aleksandra Grzywacz, Anna Laszczyńska, Maria Zimny, Małgorzata Karakiewicz, Beata Rotter, Iwona Kosik-Bogacka, Danuta Cybulski, Cezary The Relationship between the HLA-G Polymorphism and sHLA-G Levels in Parental Pairs with High-Risk Pregnancy |
title | The Relationship between the HLA-G Polymorphism and sHLA-G Levels in Parental Pairs with High-Risk Pregnancy |
title_full | The Relationship between the HLA-G Polymorphism and sHLA-G Levels in Parental Pairs with High-Risk Pregnancy |
title_fullStr | The Relationship between the HLA-G Polymorphism and sHLA-G Levels in Parental Pairs with High-Risk Pregnancy |
title_full_unstemmed | The Relationship between the HLA-G Polymorphism and sHLA-G Levels in Parental Pairs with High-Risk Pregnancy |
title_short | The Relationship between the HLA-G Polymorphism and sHLA-G Levels in Parental Pairs with High-Risk Pregnancy |
title_sort | relationship between the hla-g polymorphism and shla-g levels in parental pairs with high-risk pregnancy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539819/ https://www.ncbi.nlm.nih.gov/pubmed/31052398 http://dx.doi.org/10.3390/ijerph16091546 |
work_keys_str_mv | AT sipakolimpia therelationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT ryłaleksandra therelationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT grzywaczanna therelationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT laszczynskamaria therelationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT zimnymałgorzata therelationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT karakiewiczbeata therelationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT rotteriwona therelationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT kosikbogackadanuta therelationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT cybulskicezary therelationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT sipakolimpia relationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT ryłaleksandra relationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT grzywaczanna relationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT laszczynskamaria relationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT zimnymałgorzata relationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT karakiewiczbeata relationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT rotteriwona relationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT kosikbogackadanuta relationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy AT cybulskicezary relationshipbetweenthehlagpolymorphismandshlaglevelsinparentalpairswithhighriskpregnancy |