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Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli Isolated from Flies in the Urban Center of Berlin, Germany

Background: The monitoring of antimicrobial resistance (AMR) in microorganisms that circulate in the environment is an important topic of scientific research and contributes to the development of action plans to combat the spread of multidrug-resistant (MDR) bacteria. As a synanthropic vector for mu...

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Autores principales: Wetzker, Wibke, Pfeifer, Yvonne, Wolke, Solvy, Haselbeck, Andrea, Leistner, Rasmus, Kola, Axel, Gastmeier, Petra, Salm, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539871/
https://www.ncbi.nlm.nih.gov/pubmed/31052188
http://dx.doi.org/10.3390/ijerph16091530
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author Wetzker, Wibke
Pfeifer, Yvonne
Wolke, Solvy
Haselbeck, Andrea
Leistner, Rasmus
Kola, Axel
Gastmeier, Petra
Salm, Florian
author_facet Wetzker, Wibke
Pfeifer, Yvonne
Wolke, Solvy
Haselbeck, Andrea
Leistner, Rasmus
Kola, Axel
Gastmeier, Petra
Salm, Florian
author_sort Wetzker, Wibke
collection PubMed
description Background: The monitoring of antimicrobial resistance (AMR) in microorganisms that circulate in the environment is an important topic of scientific research and contributes to the development of action plans to combat the spread of multidrug-resistant (MDR) bacteria. As a synanthropic vector for multiple pathogens and a reservoir for AMR, flies can be used for surveillance. Methods: We collected 163 flies in the inner city of Berlin and examined them for extended-spectrum β-lactamase (ESBL)-producing Escherichia coli genotypically and phenotypically. Results: The prevalence of ESBL-producing E. coli in flies was 12.9%. Almost half (47.6%) of the ESBL-positive samples showed a co-resistance to ciprofloxacin. Resistance to carbapenems or colistin was not detected. The predominant ESBL-type was CTX-M-1, which is associated with wildlife, livestock, and companion animals as a potential major source of transmission of MDR E. coli to flies. Conclusions: This field study confirms the permanent presence of ESBL-producing E. coli in an urban fly population. For continuous monitoring of environmental contamination with multidrug-resistant (MDR) bacteria, flies can be used as indicators without much effort.
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spelling pubmed-65398712019-06-05 Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli Isolated from Flies in the Urban Center of Berlin, Germany Wetzker, Wibke Pfeifer, Yvonne Wolke, Solvy Haselbeck, Andrea Leistner, Rasmus Kola, Axel Gastmeier, Petra Salm, Florian Int J Environ Res Public Health Article Background: The monitoring of antimicrobial resistance (AMR) in microorganisms that circulate in the environment is an important topic of scientific research and contributes to the development of action plans to combat the spread of multidrug-resistant (MDR) bacteria. As a synanthropic vector for multiple pathogens and a reservoir for AMR, flies can be used for surveillance. Methods: We collected 163 flies in the inner city of Berlin and examined them for extended-spectrum β-lactamase (ESBL)-producing Escherichia coli genotypically and phenotypically. Results: The prevalence of ESBL-producing E. coli in flies was 12.9%. Almost half (47.6%) of the ESBL-positive samples showed a co-resistance to ciprofloxacin. Resistance to carbapenems or colistin was not detected. The predominant ESBL-type was CTX-M-1, which is associated with wildlife, livestock, and companion animals as a potential major source of transmission of MDR E. coli to flies. Conclusions: This field study confirms the permanent presence of ESBL-producing E. coli in an urban fly population. For continuous monitoring of environmental contamination with multidrug-resistant (MDR) bacteria, flies can be used as indicators without much effort. MDPI 2019-04-30 2019-05 /pmc/articles/PMC6539871/ /pubmed/31052188 http://dx.doi.org/10.3390/ijerph16091530 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wetzker, Wibke
Pfeifer, Yvonne
Wolke, Solvy
Haselbeck, Andrea
Leistner, Rasmus
Kola, Axel
Gastmeier, Petra
Salm, Florian
Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli Isolated from Flies in the Urban Center of Berlin, Germany
title Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli Isolated from Flies in the Urban Center of Berlin, Germany
title_full Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli Isolated from Flies in the Urban Center of Berlin, Germany
title_fullStr Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli Isolated from Flies in the Urban Center of Berlin, Germany
title_full_unstemmed Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli Isolated from Flies in the Urban Center of Berlin, Germany
title_short Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli Isolated from Flies in the Urban Center of Berlin, Germany
title_sort extended-spectrum beta-lactamase (esbl)-producing escherichia coli isolated from flies in the urban center of berlin, germany
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539871/
https://www.ncbi.nlm.nih.gov/pubmed/31052188
http://dx.doi.org/10.3390/ijerph16091530
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