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Geranylgeraniol Suppresses the Expression of IRAK1 and TRAF6 to Inhibit NFκB Activation in Lipopolysaccharide-Induced Inflammatory Responses in Human Macrophage-Like Cells

Geranylgeraniol (GGOH), a natural isoprenoid found in plants, has anti-inflammatory effects via inhibiting the activation of nuclear factor-kappa B (NFκB). However, its detailed mechanism has not yet been elucidated. Recent studies have revealed that isoprenoids can modulate signaling molecules in i...

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Autores principales: Giriwono, Puspo E., Shirakawa, Hitoshi, Ohsaki, Yusuke, Sato, Shoko, Aoyama, Yukihide, Ho, Hsin-Jung, Goto, Tomoko, Komai, Michio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6540148/
https://www.ncbi.nlm.nih.gov/pubmed/31083375
http://dx.doi.org/10.3390/ijms20092320
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author Giriwono, Puspo E.
Shirakawa, Hitoshi
Ohsaki, Yusuke
Sato, Shoko
Aoyama, Yukihide
Ho, Hsin-Jung
Goto, Tomoko
Komai, Michio
author_facet Giriwono, Puspo E.
Shirakawa, Hitoshi
Ohsaki, Yusuke
Sato, Shoko
Aoyama, Yukihide
Ho, Hsin-Jung
Goto, Tomoko
Komai, Michio
author_sort Giriwono, Puspo E.
collection PubMed
description Geranylgeraniol (GGOH), a natural isoprenoid found in plants, has anti-inflammatory effects via inhibiting the activation of nuclear factor-kappa B (NFκB). However, its detailed mechanism has not yet been elucidated. Recent studies have revealed that isoprenoids can modulate signaling molecules in innate immune responses. We found that GGOH decreased the expression of lipopolysaccharide (LPS)-induced inflammatory genes in human macrophage-like THP-1 cells. Furthermore, we observed that the suppression of NFκB signaling proteins, in particular interleukin-1 receptor-associated kinase 1 (IRAK1) and tumor necrosis factor receptor-associated factor 6 (TRAF6), occurred in GGOH-treated cells prior to LPS stimulation, suggesting an immunomodulatory effect. These results indicate that GGOH may modulate and help prevent excessive NFκB activation that can lead to numerous diseases.
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spelling pubmed-65401482019-06-04 Geranylgeraniol Suppresses the Expression of IRAK1 and TRAF6 to Inhibit NFκB Activation in Lipopolysaccharide-Induced Inflammatory Responses in Human Macrophage-Like Cells Giriwono, Puspo E. Shirakawa, Hitoshi Ohsaki, Yusuke Sato, Shoko Aoyama, Yukihide Ho, Hsin-Jung Goto, Tomoko Komai, Michio Int J Mol Sci Article Geranylgeraniol (GGOH), a natural isoprenoid found in plants, has anti-inflammatory effects via inhibiting the activation of nuclear factor-kappa B (NFκB). However, its detailed mechanism has not yet been elucidated. Recent studies have revealed that isoprenoids can modulate signaling molecules in innate immune responses. We found that GGOH decreased the expression of lipopolysaccharide (LPS)-induced inflammatory genes in human macrophage-like THP-1 cells. Furthermore, we observed that the suppression of NFκB signaling proteins, in particular interleukin-1 receptor-associated kinase 1 (IRAK1) and tumor necrosis factor receptor-associated factor 6 (TRAF6), occurred in GGOH-treated cells prior to LPS stimulation, suggesting an immunomodulatory effect. These results indicate that GGOH may modulate and help prevent excessive NFκB activation that can lead to numerous diseases. MDPI 2019-05-10 /pmc/articles/PMC6540148/ /pubmed/31083375 http://dx.doi.org/10.3390/ijms20092320 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Giriwono, Puspo E.
Shirakawa, Hitoshi
Ohsaki, Yusuke
Sato, Shoko
Aoyama, Yukihide
Ho, Hsin-Jung
Goto, Tomoko
Komai, Michio
Geranylgeraniol Suppresses the Expression of IRAK1 and TRAF6 to Inhibit NFκB Activation in Lipopolysaccharide-Induced Inflammatory Responses in Human Macrophage-Like Cells
title Geranylgeraniol Suppresses the Expression of IRAK1 and TRAF6 to Inhibit NFκB Activation in Lipopolysaccharide-Induced Inflammatory Responses in Human Macrophage-Like Cells
title_full Geranylgeraniol Suppresses the Expression of IRAK1 and TRAF6 to Inhibit NFκB Activation in Lipopolysaccharide-Induced Inflammatory Responses in Human Macrophage-Like Cells
title_fullStr Geranylgeraniol Suppresses the Expression of IRAK1 and TRAF6 to Inhibit NFκB Activation in Lipopolysaccharide-Induced Inflammatory Responses in Human Macrophage-Like Cells
title_full_unstemmed Geranylgeraniol Suppresses the Expression of IRAK1 and TRAF6 to Inhibit NFκB Activation in Lipopolysaccharide-Induced Inflammatory Responses in Human Macrophage-Like Cells
title_short Geranylgeraniol Suppresses the Expression of IRAK1 and TRAF6 to Inhibit NFκB Activation in Lipopolysaccharide-Induced Inflammatory Responses in Human Macrophage-Like Cells
title_sort geranylgeraniol suppresses the expression of irak1 and traf6 to inhibit nfκb activation in lipopolysaccharide-induced inflammatory responses in human macrophage-like cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6540148/
https://www.ncbi.nlm.nih.gov/pubmed/31083375
http://dx.doi.org/10.3390/ijms20092320
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