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Influence of intermittent fasting on myocardial infarction-induced cardiac remodeling
BACKGROUND: Information on the role of intermittent fasting (IF) on pathologic cardiac remodeling is scarce. We compared the effects of IF before and after myocardial infarction (MI) on rat cardiac remodeling and survival. METHODS: Wistar rats were intermittently fasted (food available every other d...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6540428/ https://www.ncbi.nlm.nih.gov/pubmed/31138145 http://dx.doi.org/10.1186/s12872-019-1113-4 |
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author | Okoshi, K. Cezar, M. D. M. Polin, M. A. M. Paladino, J. R. Martinez, P. F. Oliveira, S. A. Lima, A. R. R. Damatto, R. L. Paiva, S. A. R. Zornoff, L. A. M. Okoshi, M. P. |
author_facet | Okoshi, K. Cezar, M. D. M. Polin, M. A. M. Paladino, J. R. Martinez, P. F. Oliveira, S. A. Lima, A. R. R. Damatto, R. L. Paiva, S. A. R. Zornoff, L. A. M. Okoshi, M. P. |
author_sort | Okoshi, K. |
collection | PubMed |
description | BACKGROUND: Information on the role of intermittent fasting (IF) on pathologic cardiac remodeling is scarce. We compared the effects of IF before and after myocardial infarction (MI) on rat cardiac remodeling and survival. METHODS: Wistar rats were intermittently fasted (food available every other day) or fed ad libitum for 12 weeks and then divided into three groups: AL – fed ad libitum; AL/IF - fed AL before MI and IF after MI; and IF – fed IF before and after MI. Echocardiogram was performed before MI and 2 and 12 weeks after surgery. Isolated hearts were evaluated in Langendorff preparations. RESULTS: Before surgery, body weight (BW) was lower in IF than AL. Final BW was lower in AL/IF and IF than AL. Perioperative mortality did not change between AL (31.3%) and IF (27.3%). Total mortality was lower in IF than AL. Before surgery, echocardiographic parameters did not differ between groups. Two weeks after surgery, MI size did not differ between groups. Twelve weeks after MI, left ventricular (LV) diastolic posterior wall thickness was lower in AL/IF and IF than AL. The percentage of variation of echocardiographic parameters between twelve and two weeks showed that MI size decreased in all groups and the reduction was higher in IF than AL/IF. In Langendorff preparations, LV volume at zero end-diastolic pressure (V0; AL: 0.41 ± 0.05; AL/IF: 0.34 ± 0.06; IF: 0.28 ± 0.05 mL) and at 25 mmHg end-diastolic pressure (V25; AL: 0.61 ± 0.05; AL/IF: 0.54 ± 0.07; IF: 0.44 ± 0.06 mL) was lower in AL/IF and IF than AL and V25 was lower in IF than AL/IF. V0/BW ratio was lower in IF than AL and LV weight/V0 ratio was higher in IF than AL. Myocyte diameter was lower in AL/IF and IF than AL (AL: 17.3 ± 1.70; AL/IF: 15.1 ± 2.21; IF: 13.4 ± 1.49 μm). Myocardial hydroxyproline concentration and gene expression of ANP, Serca 2a, and α- and β-myosin heavy chain did not differ between groups. CONCLUSION: Intermittent fasting initiated before or after MI reduces myocyte hypertrophy and LV dilation. Myocardial fibrosis and fetal gene expression are not modulated by feeding regimens. Benefit is more evident when intermittent fasting is initiated before rather than after MI. |
format | Online Article Text |
id | pubmed-6540428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-65404282019-06-03 Influence of intermittent fasting on myocardial infarction-induced cardiac remodeling Okoshi, K. Cezar, M. D. M. Polin, M. A. M. Paladino, J. R. Martinez, P. F. Oliveira, S. A. Lima, A. R. R. Damatto, R. L. Paiva, S. A. R. Zornoff, L. A. M. Okoshi, M. P. BMC Cardiovasc Disord Research Article BACKGROUND: Information on the role of intermittent fasting (IF) on pathologic cardiac remodeling is scarce. We compared the effects of IF before and after myocardial infarction (MI) on rat cardiac remodeling and survival. METHODS: Wistar rats were intermittently fasted (food available every other day) or fed ad libitum for 12 weeks and then divided into three groups: AL – fed ad libitum; AL/IF - fed AL before MI and IF after MI; and IF – fed IF before and after MI. Echocardiogram was performed before MI and 2 and 12 weeks after surgery. Isolated hearts were evaluated in Langendorff preparations. RESULTS: Before surgery, body weight (BW) was lower in IF than AL. Final BW was lower in AL/IF and IF than AL. Perioperative mortality did not change between AL (31.3%) and IF (27.3%). Total mortality was lower in IF than AL. Before surgery, echocardiographic parameters did not differ between groups. Two weeks after surgery, MI size did not differ between groups. Twelve weeks after MI, left ventricular (LV) diastolic posterior wall thickness was lower in AL/IF and IF than AL. The percentage of variation of echocardiographic parameters between twelve and two weeks showed that MI size decreased in all groups and the reduction was higher in IF than AL/IF. In Langendorff preparations, LV volume at zero end-diastolic pressure (V0; AL: 0.41 ± 0.05; AL/IF: 0.34 ± 0.06; IF: 0.28 ± 0.05 mL) and at 25 mmHg end-diastolic pressure (V25; AL: 0.61 ± 0.05; AL/IF: 0.54 ± 0.07; IF: 0.44 ± 0.06 mL) was lower in AL/IF and IF than AL and V25 was lower in IF than AL/IF. V0/BW ratio was lower in IF than AL and LV weight/V0 ratio was higher in IF than AL. Myocyte diameter was lower in AL/IF and IF than AL (AL: 17.3 ± 1.70; AL/IF: 15.1 ± 2.21; IF: 13.4 ± 1.49 μm). Myocardial hydroxyproline concentration and gene expression of ANP, Serca 2a, and α- and β-myosin heavy chain did not differ between groups. CONCLUSION: Intermittent fasting initiated before or after MI reduces myocyte hypertrophy and LV dilation. Myocardial fibrosis and fetal gene expression are not modulated by feeding regimens. Benefit is more evident when intermittent fasting is initiated before rather than after MI. BioMed Central 2019-05-28 /pmc/articles/PMC6540428/ /pubmed/31138145 http://dx.doi.org/10.1186/s12872-019-1113-4 Text en © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Okoshi, K. Cezar, M. D. M. Polin, M. A. M. Paladino, J. R. Martinez, P. F. Oliveira, S. A. Lima, A. R. R. Damatto, R. L. Paiva, S. A. R. Zornoff, L. A. M. Okoshi, M. P. Influence of intermittent fasting on myocardial infarction-induced cardiac remodeling |
title | Influence of intermittent fasting on myocardial infarction-induced cardiac remodeling |
title_full | Influence of intermittent fasting on myocardial infarction-induced cardiac remodeling |
title_fullStr | Influence of intermittent fasting on myocardial infarction-induced cardiac remodeling |
title_full_unstemmed | Influence of intermittent fasting on myocardial infarction-induced cardiac remodeling |
title_short | Influence of intermittent fasting on myocardial infarction-induced cardiac remodeling |
title_sort | influence of intermittent fasting on myocardial infarction-induced cardiac remodeling |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6540428/ https://www.ncbi.nlm.nih.gov/pubmed/31138145 http://dx.doi.org/10.1186/s12872-019-1113-4 |
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