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Development of a novel strategy for robust synthetic bacterial promoters based on a stepwise evolution targeting the spacer region of the core promoter in Bacillus subtilis

BACKGROUND: Promoter evolution by synthetic promoter library (SPL) is a powerful approach to development of functional synthetic promoters to synthetic biology. However, it requires much tedious and time-consuming screenings because of the plethora of different variants in SPL. Actually, a large pro...

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Detalles Bibliográficos
Autores principales: Han, Laichuang, Cui, Wenjing, Suo, Feiya, Miao, Shengnan, Hao, Wenliang, Chen, Qiaoqing, Guo, Junling, Liu, Zhongmei, Zhou, Li, Zhou, Zhemin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6540529/
https://www.ncbi.nlm.nih.gov/pubmed/31142347
http://dx.doi.org/10.1186/s12934-019-1148-3