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Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency

Mouse embryonic stem cells (mESCs) can be maintained as homogeneous populations in the ground state of pluripotency. Release from this state in minimal conditions allows to obtain cells that resemble those of the early post-implantation epiblast, providing an important developmental model to study c...

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Autores principales: Waisman, Ariel, Sevlever, Federico, Elías Costa, Martín, Cosentino, María Soledad, Miriuka, Santiago G., Ventura, Alejandra C., Guberman, Alejandra S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541595/
https://www.ncbi.nlm.nih.gov/pubmed/31142785
http://dx.doi.org/10.1038/s41598-019-44537-0
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author Waisman, Ariel
Sevlever, Federico
Elías Costa, Martín
Cosentino, María Soledad
Miriuka, Santiago G.
Ventura, Alejandra C.
Guberman, Alejandra S.
author_facet Waisman, Ariel
Sevlever, Federico
Elías Costa, Martín
Cosentino, María Soledad
Miriuka, Santiago G.
Ventura, Alejandra C.
Guberman, Alejandra S.
author_sort Waisman, Ariel
collection PubMed
description Mouse embryonic stem cells (mESCs) can be maintained as homogeneous populations in the ground state of pluripotency. Release from this state in minimal conditions allows to obtain cells that resemble those of the early post-implantation epiblast, providing an important developmental model to study cell identity transitions. However, the cell cycle dynamics of mESCs in the ground state and during its dissolution have not been extensively studied. By performing live imaging experiments of mESCs bearing cell cycle reporters, we show here that cells in the pluripotent ground state display a cell cycle structure comparable to the reported for mESCs in serum-based media. Upon release from self-renewal, the cell cycle is rapidly accelerated by a reduction in the length of the G1 phase and of the S/G2/M phases, causing an increased proliferation rate. Analysis of cell lineages indicates that cell cycle variables of sister cells are highly correlated, suggesting the existence of inherited cell cycle regulators from the parental cell. Together with a major morphological reconfiguration upon differentiation, our findings support a correlation between this in vitro model and early embryonic events.
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spelling pubmed-65415952019-06-07 Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency Waisman, Ariel Sevlever, Federico Elías Costa, Martín Cosentino, María Soledad Miriuka, Santiago G. Ventura, Alejandra C. Guberman, Alejandra S. Sci Rep Article Mouse embryonic stem cells (mESCs) can be maintained as homogeneous populations in the ground state of pluripotency. Release from this state in minimal conditions allows to obtain cells that resemble those of the early post-implantation epiblast, providing an important developmental model to study cell identity transitions. However, the cell cycle dynamics of mESCs in the ground state and during its dissolution have not been extensively studied. By performing live imaging experiments of mESCs bearing cell cycle reporters, we show here that cells in the pluripotent ground state display a cell cycle structure comparable to the reported for mESCs in serum-based media. Upon release from self-renewal, the cell cycle is rapidly accelerated by a reduction in the length of the G1 phase and of the S/G2/M phases, causing an increased proliferation rate. Analysis of cell lineages indicates that cell cycle variables of sister cells are highly correlated, suggesting the existence of inherited cell cycle regulators from the parental cell. Together with a major morphological reconfiguration upon differentiation, our findings support a correlation between this in vitro model and early embryonic events. Nature Publishing Group UK 2019-05-29 /pmc/articles/PMC6541595/ /pubmed/31142785 http://dx.doi.org/10.1038/s41598-019-44537-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Waisman, Ariel
Sevlever, Federico
Elías Costa, Martín
Cosentino, María Soledad
Miriuka, Santiago G.
Ventura, Alejandra C.
Guberman, Alejandra S.
Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency
title Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency
title_full Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency
title_fullStr Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency
title_full_unstemmed Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency
title_short Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency
title_sort cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541595/
https://www.ncbi.nlm.nih.gov/pubmed/31142785
http://dx.doi.org/10.1038/s41598-019-44537-0
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