Sex identification using ZFX and ZFY genes in leopard cats (Prionailurus bengalensis euptilurus) in Korea

In this study, we used multiplex polymerase chain reaction (PCR) to determine the sex of leopard cats (Prionailurus bengalensis euptilurus) in Korea. When we applied PCR using primers based on the intronic region between ZFX and ZFY, only one DNA band of 143 bp was detected in females, compared with...

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Detalles Bibliográficos
Autores principales: SEOK, Seong Hoon, KANG, Sun Young, IM, Young Bin, YOO, Han Sang, YEON, Seong-Chan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2019
Materias:
Wildlife Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541836/
https://www.ncbi.nlm.nih.gov/pubmed/30944271
http://dx.doi.org/10.1292/jvms.18-0693
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author SEOK, Seong Hoon
KANG, Sun Young
IM, Young Bin
YOO, Han Sang
YEON, Seong-Chan
author_facet SEOK, Seong Hoon
KANG, Sun Young
IM, Young Bin
YOO, Han Sang
YEON, Seong-Chan
author_sort SEOK, Seong Hoon
collection PubMed
description In this study, we used multiplex polymerase chain reaction (PCR) to determine the sex of leopard cats (Prionailurus bengalensis euptilurus) in Korea. When we applied PCR using primers based on the intronic region between ZFX and ZFY, only one DNA band of 143 bp was detected in females, compared with two specific bands of 143 and 310 bp in males, indicating that the band patterns were clearly different between females and males. Since the set of primers also proved compatible with tissue and fecal samples, the results of the present study demonstrate that the present PCR could be a convenient tool for identifying the sex of leopard cats.
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spelling pubmed-65418362019-06-11 Sex identification using ZFX and ZFY genes in leopard cats (Prionailurus bengalensis euptilurus) in Korea SEOK, Seong Hoon KANG, Sun Young IM, Young Bin YOO, Han Sang YEON, Seong-Chan J Vet Med Sci Wildlife Science In this study, we used multiplex polymerase chain reaction (PCR) to determine the sex of leopard cats (Prionailurus bengalensis euptilurus) in Korea. When we applied PCR using primers based on the intronic region between ZFX and ZFY, only one DNA band of 143 bp was detected in females, compared with two specific bands of 143 and 310 bp in males, indicating that the band patterns were clearly different between females and males. Since the set of primers also proved compatible with tissue and fecal samples, the results of the present study demonstrate that the present PCR could be a convenient tool for identifying the sex of leopard cats. The Japanese Society of Veterinary Science 2019-04-02 2019-05 /pmc/articles/PMC6541836/ /pubmed/30944271 http://dx.doi.org/10.1292/jvms.18-0693 Text en ©2019 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Wildlife Science
SEOK, Seong Hoon
KANG, Sun Young
IM, Young Bin
YOO, Han Sang
YEON, Seong-Chan
Sex identification using ZFX and ZFY genes in leopard cats (Prionailurus bengalensis euptilurus) in Korea
title Sex identification using ZFX and ZFY genes in leopard cats (Prionailurus bengalensis euptilurus) in Korea
title_full Sex identification using ZFX and ZFY genes in leopard cats (Prionailurus bengalensis euptilurus) in Korea
title_fullStr Sex identification using ZFX and ZFY genes in leopard cats (Prionailurus bengalensis euptilurus) in Korea
title_full_unstemmed Sex identification using ZFX and ZFY genes in leopard cats (Prionailurus bengalensis euptilurus) in Korea
title_short Sex identification using ZFX and ZFY genes in leopard cats (Prionailurus bengalensis euptilurus) in Korea
title_sort sex identification using zfx and zfy genes in leopard cats (prionailurus bengalensis euptilurus) in korea
topic Wildlife Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541836/
https://www.ncbi.nlm.nih.gov/pubmed/30944271
http://dx.doi.org/10.1292/jvms.18-0693
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