Development of a fluorescent loop-mediated isothermal amplification assay for rapid and simple diagnosis of bovine leukemia virus infection

Bovine leukemia virus (BLV) causes enzootic bovine leukosis (EBL), a condition that threatens the sustainability of the livestock industry. A fluorescent loop-mediated isothermal amplification (fLAMP) assay targeting BLV env sequences was developed and used to evaluate 100 bovine blood samples. Comp...

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Autores principales: NAGAO, Konomu, MAKINO, Ryohei, APEGO, Francis Victor, MEKATA, Hirohisa, YAMAZAKI, Wataru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2019
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541838/
https://www.ncbi.nlm.nih.gov/pubmed/30918136
http://dx.doi.org/10.1292/jvms.19-0009
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author NAGAO, Konomu
MAKINO, Ryohei
APEGO, Francis Victor
MEKATA, Hirohisa
YAMAZAKI, Wataru
author_facet NAGAO, Konomu
MAKINO, Ryohei
APEGO, Francis Victor
MEKATA, Hirohisa
YAMAZAKI, Wataru
author_sort NAGAO, Konomu
collection PubMed
description Bovine leukemia virus (BLV) causes enzootic bovine leukosis (EBL), a condition that threatens the sustainability of the livestock industry. A fluorescent loop-mediated isothermal amplification (fLAMP) assay targeting BLV env sequences was developed and used to evaluate 100 bovine blood samples. Compared with a conventional real-time PCR (rPCR) assay, the fLAMP assay achieved 87.3% (62/71) sensitivity and 100% (29/29) specificity. The rPCR assay took 65 min, while the fLAMP assay took 8 min to 30 min from the beginning of DNA amplification to final judgement with a comparable limit of detection. The fLAMP is a potential tool for the rapid and simple diagnosis of BLV infection to supplement ELISA testing and can be used by local laboratories and slaughterhouses without special equipment.
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spelling pubmed-65418382019-06-11 Development of a fluorescent loop-mediated isothermal amplification assay for rapid and simple diagnosis of bovine leukemia virus infection NAGAO, Konomu MAKINO, Ryohei APEGO, Francis Victor MEKATA, Hirohisa YAMAZAKI, Wataru J Vet Med Sci Virology Bovine leukemia virus (BLV) causes enzootic bovine leukosis (EBL), a condition that threatens the sustainability of the livestock industry. A fluorescent loop-mediated isothermal amplification (fLAMP) assay targeting BLV env sequences was developed and used to evaluate 100 bovine blood samples. Compared with a conventional real-time PCR (rPCR) assay, the fLAMP assay achieved 87.3% (62/71) sensitivity and 100% (29/29) specificity. The rPCR assay took 65 min, while the fLAMP assay took 8 min to 30 min from the beginning of DNA amplification to final judgement with a comparable limit of detection. The fLAMP is a potential tool for the rapid and simple diagnosis of BLV infection to supplement ELISA testing and can be used by local laboratories and slaughterhouses without special equipment. The Japanese Society of Veterinary Science 2019-03-27 2019-05 /pmc/articles/PMC6541838/ /pubmed/30918136 http://dx.doi.org/10.1292/jvms.19-0009 Text en ©2019 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Virology
NAGAO, Konomu
MAKINO, Ryohei
APEGO, Francis Victor
MEKATA, Hirohisa
YAMAZAKI, Wataru
Development of a fluorescent loop-mediated isothermal amplification assay for rapid and simple diagnosis of bovine leukemia virus infection
title Development of a fluorescent loop-mediated isothermal amplification assay for rapid and simple diagnosis of bovine leukemia virus infection
title_full Development of a fluorescent loop-mediated isothermal amplification assay for rapid and simple diagnosis of bovine leukemia virus infection
title_fullStr Development of a fluorescent loop-mediated isothermal amplification assay for rapid and simple diagnosis of bovine leukemia virus infection
title_full_unstemmed Development of a fluorescent loop-mediated isothermal amplification assay for rapid and simple diagnosis of bovine leukemia virus infection
title_short Development of a fluorescent loop-mediated isothermal amplification assay for rapid and simple diagnosis of bovine leukemia virus infection
title_sort development of a fluorescent loop-mediated isothermal amplification assay for rapid and simple diagnosis of bovine leukemia virus infection
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541838/
https://www.ncbi.nlm.nih.gov/pubmed/30918136
http://dx.doi.org/10.1292/jvms.19-0009
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