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Exogenous Hydrogen Sulfide Regulates the Growth of Human Thyroid Carcinoma Cells

Hydrogen sulfide (H(2)S) is involved in the development and progression of many types of cancer. However, the effect and mechanism of H(2)S on the growth of human thyroid carcinoma cells remain unknown. In the present study, we found that the proliferation, viability, migration, and invasion of huma...

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Autores principales: Wu, Dongdong, Li, Jianmei, Zhang, Qianqian, Tian, Wenke, Zhong, Peiyu, Liu, Zhengguo, Wang, Huijuan, Wang, Honggang, Ji, Ailing, Li, Yanzhang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541980/
https://www.ncbi.nlm.nih.gov/pubmed/31223424
http://dx.doi.org/10.1155/2019/6927298
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author Wu, Dongdong
Li, Jianmei
Zhang, Qianqian
Tian, Wenke
Zhong, Peiyu
Liu, Zhengguo
Wang, Huijuan
Wang, Honggang
Ji, Ailing
Li, Yanzhang
author_facet Wu, Dongdong
Li, Jianmei
Zhang, Qianqian
Tian, Wenke
Zhong, Peiyu
Liu, Zhengguo
Wang, Huijuan
Wang, Honggang
Ji, Ailing
Li, Yanzhang
author_sort Wu, Dongdong
collection PubMed
description Hydrogen sulfide (H(2)S) is involved in the development and progression of many types of cancer. However, the effect and mechanism of H(2)S on the growth of human thyroid carcinoma cells remain unknown. In the present study, we found that the proliferation, viability, migration, and invasion of human thyroid carcinoma cells were enhanced by 25–50 μM NaHS (an H(2)S donor) and inhibited by 200 μM NaHS. However, H(2)S showed no obvious effects on the proliferation, viability, and migration of human normal thyroid cells. Administration of 50 μM NaHS increased the expression levels of CBS, SQR, and TST, while 200 μM NaHS showed reverse effects in human thyroid carcinoma cells. After treatment with 25-50 μM NaHS, the ROS levels were decreased and the protein levels of p-PI3K, p-AKT, p-mTOR, H-RAS, p-RAF, p-MEK1/2, and p-ERK1/2 were increased, whereas 200 μM NaHS exerted opposite effects in human thyroid carcinoma cells. Furthermore, 1.4-2.8 mg/kg/day NaHS promoted the tumor growth and blood vessel formation in human thyroid carcinoma xenograft tumors, while 11.2 mg/kg/day NaHS inhibited the tumor growth and angiogenesis. In conclusion, our results demonstrate that exogenous H(2)S regulates the growth of human thyroid carcinoma cells through ROS/PI3K/Akt/mTOR and RAS/RAF/MEK/ERK signaling pathways. Novel H(2)S-releasing donors/drugs can be designed and applied for the treatment of thyroid cancer.
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spelling pubmed-65419802019-06-20 Exogenous Hydrogen Sulfide Regulates the Growth of Human Thyroid Carcinoma Cells Wu, Dongdong Li, Jianmei Zhang, Qianqian Tian, Wenke Zhong, Peiyu Liu, Zhengguo Wang, Huijuan Wang, Honggang Ji, Ailing Li, Yanzhang Oxid Med Cell Longev Research Article Hydrogen sulfide (H(2)S) is involved in the development and progression of many types of cancer. However, the effect and mechanism of H(2)S on the growth of human thyroid carcinoma cells remain unknown. In the present study, we found that the proliferation, viability, migration, and invasion of human thyroid carcinoma cells were enhanced by 25–50 μM NaHS (an H(2)S donor) and inhibited by 200 μM NaHS. However, H(2)S showed no obvious effects on the proliferation, viability, and migration of human normal thyroid cells. Administration of 50 μM NaHS increased the expression levels of CBS, SQR, and TST, while 200 μM NaHS showed reverse effects in human thyroid carcinoma cells. After treatment with 25-50 μM NaHS, the ROS levels were decreased and the protein levels of p-PI3K, p-AKT, p-mTOR, H-RAS, p-RAF, p-MEK1/2, and p-ERK1/2 were increased, whereas 200 μM NaHS exerted opposite effects in human thyroid carcinoma cells. Furthermore, 1.4-2.8 mg/kg/day NaHS promoted the tumor growth and blood vessel formation in human thyroid carcinoma xenograft tumors, while 11.2 mg/kg/day NaHS inhibited the tumor growth and angiogenesis. In conclusion, our results demonstrate that exogenous H(2)S regulates the growth of human thyroid carcinoma cells through ROS/PI3K/Akt/mTOR and RAS/RAF/MEK/ERK signaling pathways. Novel H(2)S-releasing donors/drugs can be designed and applied for the treatment of thyroid cancer. Hindawi 2019-05-16 /pmc/articles/PMC6541980/ /pubmed/31223424 http://dx.doi.org/10.1155/2019/6927298 Text en Copyright © 2019 Dongdong Wu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wu, Dongdong
Li, Jianmei
Zhang, Qianqian
Tian, Wenke
Zhong, Peiyu
Liu, Zhengguo
Wang, Huijuan
Wang, Honggang
Ji, Ailing
Li, Yanzhang
Exogenous Hydrogen Sulfide Regulates the Growth of Human Thyroid Carcinoma Cells
title Exogenous Hydrogen Sulfide Regulates the Growth of Human Thyroid Carcinoma Cells
title_full Exogenous Hydrogen Sulfide Regulates the Growth of Human Thyroid Carcinoma Cells
title_fullStr Exogenous Hydrogen Sulfide Regulates the Growth of Human Thyroid Carcinoma Cells
title_full_unstemmed Exogenous Hydrogen Sulfide Regulates the Growth of Human Thyroid Carcinoma Cells
title_short Exogenous Hydrogen Sulfide Regulates the Growth of Human Thyroid Carcinoma Cells
title_sort exogenous hydrogen sulfide regulates the growth of human thyroid carcinoma cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541980/
https://www.ncbi.nlm.nih.gov/pubmed/31223424
http://dx.doi.org/10.1155/2019/6927298
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