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A 4-Week Repeated Oral Dose Toxicity Study of Ssanghwa-Tang in Crl:CD Sprague Dawley Rats

Ssanghwa-tang (SHT), a traditional herbal formula, has been widely used to recover fatigue or consumptive disease after an illness. Along with much attention to herbal formula, the concerns about the safety and toxicity have arisen. To establish the safety information, SHT was administrated in Crl:C...

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Autores principales: Yoo, Sae-Rom, Ha, Hyekyung, Lee, Mee-Young, Shin, Hyeun-kyoo, Han, Su-Cheol, Seo, Chang-Seob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541986/
https://www.ncbi.nlm.nih.gov/pubmed/31223325
http://dx.doi.org/10.1155/2019/2135351
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author Yoo, Sae-Rom
Ha, Hyekyung
Lee, Mee-Young
Shin, Hyeun-kyoo
Han, Su-Cheol
Seo, Chang-Seob
author_facet Yoo, Sae-Rom
Ha, Hyekyung
Lee, Mee-Young
Shin, Hyeun-kyoo
Han, Su-Cheol
Seo, Chang-Seob
author_sort Yoo, Sae-Rom
collection PubMed
description Ssanghwa-tang (SHT), a traditional herbal formula, has been widely used to recover fatigue or consumptive disease after an illness. Along with much attention to herbal formula, the concerns about the safety and toxicity have arisen. To establish the safety information, SHT was administrated in Crl:CD Sprague Dawley rats at a daily dose of 0, 1000, 2000, and 5000 mg/kg for 4 weeks. During the test periods, we examined the mortality, clinical observation, body weight change, food consumption, organ weights, hematology, serum biochemistry, and urinalysis parameters. No changes of mortality and necropsy findings occurred in any of the groups during the experimental period. In either sex of rats treated with SHT at 5000 mg/kg/day, changes were observed in food intake, reticulocyte, total bilirubin, some urinalysis parameters, and relative organ weights. The results indicated that SHT did not induce toxic effects at a dose level up to 2000 mg/kg in rats. This dosage was considered no observed adverse effect level (NOAEL) and was appropriate for a 13-week subchronic toxicity study.
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spelling pubmed-65419862019-06-20 A 4-Week Repeated Oral Dose Toxicity Study of Ssanghwa-Tang in Crl:CD Sprague Dawley Rats Yoo, Sae-Rom Ha, Hyekyung Lee, Mee-Young Shin, Hyeun-kyoo Han, Su-Cheol Seo, Chang-Seob Evid Based Complement Alternat Med Research Article Ssanghwa-tang (SHT), a traditional herbal formula, has been widely used to recover fatigue or consumptive disease after an illness. Along with much attention to herbal formula, the concerns about the safety and toxicity have arisen. To establish the safety information, SHT was administrated in Crl:CD Sprague Dawley rats at a daily dose of 0, 1000, 2000, and 5000 mg/kg for 4 weeks. During the test periods, we examined the mortality, clinical observation, body weight change, food consumption, organ weights, hematology, serum biochemistry, and urinalysis parameters. No changes of mortality and necropsy findings occurred in any of the groups during the experimental period. In either sex of rats treated with SHT at 5000 mg/kg/day, changes were observed in food intake, reticulocyte, total bilirubin, some urinalysis parameters, and relative organ weights. The results indicated that SHT did not induce toxic effects at a dose level up to 2000 mg/kg in rats. This dosage was considered no observed adverse effect level (NOAEL) and was appropriate for a 13-week subchronic toxicity study. Hindawi 2019-05-16 /pmc/articles/PMC6541986/ /pubmed/31223325 http://dx.doi.org/10.1155/2019/2135351 Text en Copyright © 2019 Sae-Rom Yoo et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yoo, Sae-Rom
Ha, Hyekyung
Lee, Mee-Young
Shin, Hyeun-kyoo
Han, Su-Cheol
Seo, Chang-Seob
A 4-Week Repeated Oral Dose Toxicity Study of Ssanghwa-Tang in Crl:CD Sprague Dawley Rats
title A 4-Week Repeated Oral Dose Toxicity Study of Ssanghwa-Tang in Crl:CD Sprague Dawley Rats
title_full A 4-Week Repeated Oral Dose Toxicity Study of Ssanghwa-Tang in Crl:CD Sprague Dawley Rats
title_fullStr A 4-Week Repeated Oral Dose Toxicity Study of Ssanghwa-Tang in Crl:CD Sprague Dawley Rats
title_full_unstemmed A 4-Week Repeated Oral Dose Toxicity Study of Ssanghwa-Tang in Crl:CD Sprague Dawley Rats
title_short A 4-Week Repeated Oral Dose Toxicity Study of Ssanghwa-Tang in Crl:CD Sprague Dawley Rats
title_sort 4-week repeated oral dose toxicity study of ssanghwa-tang in crl:cd sprague dawley rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541986/
https://www.ncbi.nlm.nih.gov/pubmed/31223325
http://dx.doi.org/10.1155/2019/2135351
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