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Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, Curcumae rhizoma and Sparganii rhizoma
The aim of this study was to elucidate the concise effects of a traditional herb pair, Curcumae rhizoma-Sparganii rhizoma (CRSR), on uterine leiomyoma (UL) by analyzing transcriptional profiling. The UL rat model was made by intramuscular injection of progesterone and gavage administration of diethy...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Associação Brasileira de Divulgação Científica
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542090/ https://www.ncbi.nlm.nih.gov/pubmed/31141088 http://dx.doi.org/10.1590/1414-431X20198132 |
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author | Yu, Cheng Hao Zhao, Jin Shuang Zhao, Hui Peng, Teng Shen, Dong Cheng Xu, Qiu Xia Li, Yao Webb, R. Clinton Wang, Mong Heng Shi, Xing Ming Peng, Cheng Ding, Wei Jun |
author_facet | Yu, Cheng Hao Zhao, Jin Shuang Zhao, Hui Peng, Teng Shen, Dong Cheng Xu, Qiu Xia Li, Yao Webb, R. Clinton Wang, Mong Heng Shi, Xing Ming Peng, Cheng Ding, Wei Jun |
author_sort | Yu, Cheng Hao |
collection | PubMed |
description | The aim of this study was to elucidate the concise effects of a traditional herb pair, Curcumae rhizoma-Sparganii rhizoma (CRSR), on uterine leiomyoma (UL) by analyzing transcriptional profiling. The UL rat model was made by intramuscular injection of progesterone and gavage administration of diethylstilbestrol. From 11 weeks of the establishment of the model, rats of the UL+CRSR group were gavaged daily with CRSR (6.67 g/kg). The serum concentrations of progesterone (P) and estradiol (E(2)) were determined by radioimmunoassay, the uterine index was measured by caliper measurement, and the pathological status was observed by hematoxylin and eosin stain. Gene expression profiling was checked by NimbleGen Rat Gene Expression Microarrays. The results indicated that the uterine mass of UL+CRSR rats was significantly shrunk and serum P and E(2) levels significantly reduced compared to UL animals and nearly to the level of normal rats. Results of microarrays displayed the extensive inhibition of CRSR upon the expression of proliferation and deposition of extracellular matrix (ECM)-related genes, and significantly regulated a wide range of metabolism disorders. Furthermore, CRSR extensively regulated key pathways of the UL process, such as MAPK, PPAR, Notch, and TGF-β/Smad. Regulation of the crucial pathways for the UL process and ECM metabolism may be the underlying mechanisms of CRSR treatment. Further studies will provide clear clues for effectively treating UL with CRSR. |
format | Online Article Text |
id | pubmed-6542090 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Associação Brasileira de Divulgação Científica |
record_format | MEDLINE/PubMed |
spelling | pubmed-65420902019-06-14 Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, Curcumae rhizoma and Sparganii rhizoma Yu, Cheng Hao Zhao, Jin Shuang Zhao, Hui Peng, Teng Shen, Dong Cheng Xu, Qiu Xia Li, Yao Webb, R. Clinton Wang, Mong Heng Shi, Xing Ming Peng, Cheng Ding, Wei Jun Braz J Med Biol Res Research Article The aim of this study was to elucidate the concise effects of a traditional herb pair, Curcumae rhizoma-Sparganii rhizoma (CRSR), on uterine leiomyoma (UL) by analyzing transcriptional profiling. The UL rat model was made by intramuscular injection of progesterone and gavage administration of diethylstilbestrol. From 11 weeks of the establishment of the model, rats of the UL+CRSR group were gavaged daily with CRSR (6.67 g/kg). The serum concentrations of progesterone (P) and estradiol (E(2)) were determined by radioimmunoassay, the uterine index was measured by caliper measurement, and the pathological status was observed by hematoxylin and eosin stain. Gene expression profiling was checked by NimbleGen Rat Gene Expression Microarrays. The results indicated that the uterine mass of UL+CRSR rats was significantly shrunk and serum P and E(2) levels significantly reduced compared to UL animals and nearly to the level of normal rats. Results of microarrays displayed the extensive inhibition of CRSR upon the expression of proliferation and deposition of extracellular matrix (ECM)-related genes, and significantly regulated a wide range of metabolism disorders. Furthermore, CRSR extensively regulated key pathways of the UL process, such as MAPK, PPAR, Notch, and TGF-β/Smad. Regulation of the crucial pathways for the UL process and ECM metabolism may be the underlying mechanisms of CRSR treatment. Further studies will provide clear clues for effectively treating UL with CRSR. Associação Brasileira de Divulgação Científica 2019-05-27 /pmc/articles/PMC6542090/ /pubmed/31141088 http://dx.doi.org/10.1590/1414-431X20198132 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yu, Cheng Hao Zhao, Jin Shuang Zhao, Hui Peng, Teng Shen, Dong Cheng Xu, Qiu Xia Li, Yao Webb, R. Clinton Wang, Mong Heng Shi, Xing Ming Peng, Cheng Ding, Wei Jun Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, Curcumae rhizoma and Sparganii rhizoma |
title | Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, Curcumae rhizoma and Sparganii rhizoma
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title_full | Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, Curcumae rhizoma and Sparganii rhizoma
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title_fullStr | Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, Curcumae rhizoma and Sparganii rhizoma
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title_full_unstemmed | Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, Curcumae rhizoma and Sparganii rhizoma
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title_short | Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, Curcumae rhizoma and Sparganii rhizoma
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title_sort | transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, curcumae rhizoma and sparganii rhizoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542090/ https://www.ncbi.nlm.nih.gov/pubmed/31141088 http://dx.doi.org/10.1590/1414-431X20198132 |
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