The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells

AIM: To investigated the activities of chloroform fractions at pH 7 of Litsea cubeba Lour. Fruits and heartwoods (CF-7F and CF-7H) in decrease expression of PI3KCA, Akt-1 and Akt-2 genes towards cervical cancer cell culture (HeLa) experiments in vitro. MATERIAL AND METHODS: CF-7F and CF-7H (12.5 and...

Descripción completa

Detalles Bibliográficos
Autores principales: Dalimunthe, Aminah, Hasibuan, Poppy Anjelisa Zaitun, Satria, Denny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Republic of Macedonia 2019
Materias:
Basic Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542386/
https://www.ncbi.nlm.nih.gov/pubmed/31198447
http://dx.doi.org/10.3889/oamjms.2019.317
_version_ 1783422922714513408
author Dalimunthe, Aminah
Hasibuan, Poppy Anjelisa Zaitun
Satria, Denny
author_facet Dalimunthe, Aminah
Hasibuan, Poppy Anjelisa Zaitun
Satria, Denny
author_sort Dalimunthe, Aminah
collection PubMed
description AIM: To investigated the activities of chloroform fractions at pH 7 of Litsea cubeba Lour. Fruits and heartwoods (CF-7F and CF-7H) in decrease expression of PI3KCA, Akt-1 and Akt-2 genes towards cervical cancer cell culture (HeLa) experiments in vitro. MATERIAL AND METHODS: CF-7F and CF-7H (12.5 and 25 µg/mL) were tested for its potential inhibition on gene expression of PI3KCA, Akt-1 and Akt-2 genes by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method. RESULT: CF-7F and CF-7H were showed the activity to reduce the expression of PI3KCA, Akt-1 and Akt-2 genes. CONCLUSION: Our results suggest that CF-7F and CF-7H significantly inhibit the expression of PI3KCA, Akt-1 and Akt-2 genes.
format Online
Article
Text
id pubmed-6542386
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Republic of Macedonia
record_format MEDLINE/PubMed
spelling pubmed-65423862019-06-13 The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells Dalimunthe, Aminah Hasibuan, Poppy Anjelisa Zaitun Satria, Denny Open Access Maced J Med Sci Basic Science AIM: To investigated the activities of chloroform fractions at pH 7 of Litsea cubeba Lour. Fruits and heartwoods (CF-7F and CF-7H) in decrease expression of PI3KCA, Akt-1 and Akt-2 genes towards cervical cancer cell culture (HeLa) experiments in vitro. MATERIAL AND METHODS: CF-7F and CF-7H (12.5 and 25 µg/mL) were tested for its potential inhibition on gene expression of PI3KCA, Akt-1 and Akt-2 genes by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method. RESULT: CF-7F and CF-7H were showed the activity to reduce the expression of PI3KCA, Akt-1 and Akt-2 genes. CONCLUSION: Our results suggest that CF-7F and CF-7H significantly inhibit the expression of PI3KCA, Akt-1 and Akt-2 genes. Republic of Macedonia 2019-05-14 /pmc/articles/PMC6542386/ /pubmed/31198447 http://dx.doi.org/10.3889/oamjms.2019.317 Text en Copyright: © 2019 Aminah Dalimunthe, Poppy Anjelisa Zaitun Hasibuan, Denny Satria. http://creativecommons.org/licenses/CC BY-NC/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
spellingShingle Basic Science
Dalimunthe, Aminah
Hasibuan, Poppy Anjelisa Zaitun
Satria, Denny
The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells
title The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells
title_full The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells
title_fullStr The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells
title_full_unstemmed The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells
title_short The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells
title_sort pi3kca and akt inhibitory activities of litsea cubeba lour. fruits and heartwoods towards hela cells
topic Basic Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542386/
https://www.ncbi.nlm.nih.gov/pubmed/31198447
http://dx.doi.org/10.3889/oamjms.2019.317
work_keys_str_mv AT dalimuntheaminah thepi3kcaandaktinhibitoryactivitiesoflitseacubebalourfruitsandheartwoodstowardshelacells
AT hasibuanpoppyanjelisazaitun thepi3kcaandaktinhibitoryactivitiesoflitseacubebalourfruitsandheartwoodstowardshelacells
AT satriadenny thepi3kcaandaktinhibitoryactivitiesoflitseacubebalourfruitsandheartwoodstowardshelacells
AT dalimuntheaminah pi3kcaandaktinhibitoryactivitiesoflitseacubebalourfruitsandheartwoodstowardshelacells
AT hasibuanpoppyanjelisazaitun pi3kcaandaktinhibitoryactivitiesoflitseacubebalourfruitsandheartwoodstowardshelacells
AT satriadenny pi3kcaandaktinhibitoryactivitiesoflitseacubebalourfruitsandheartwoodstowardshelacells

Ejemplares similares