Cargando…

Reversible association with motor proteins (RAMP): A streptavidin-based method to manipulate organelle positioning

We report the development and characterization of a method, named reversible association with motor proteins (RAMP), for manipulation of organelle positioning within the cytoplasm. RAMP consists of coexpressing in cultured cells (i) an organellar protein fused to the streptavidin-binding peptide (SB...

Descripción completa

Detalles Bibliográficos
Autores principales:	Guardia, Carlos M., De Pace, Raffaella, Sen, Aritra, Saric, Amra, Jarnik, Michal, Kolin, David A., Kunwar, Ambarish, Bonifacino, Juan S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2019
Materias:	Methods and Resources
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542540/ https://www.ncbi.nlm.nih.gov/pubmed/31100061 http://dx.doi.org/10.1371/journal.pbio.3000279

Ejemplares similares

Protease‐resistant streptavidin for interaction proteomics
por: Rafiee, Mahmoud‐Reza, et al.
Publicado: (2020)

In situ artificial contact sites (ISACS) between synthetic and endogenous organelle membranes allow for quantification of protein-tethering activities
por: Milanini, Julie, et al.
Publicado: (2022)

In situ characterisation and manipulation of biological systems with Chi.Bio
por: Steel, Harrison, et al.
Publicado: (2020)

SNX19 restricts endolysosome motility through contacts with the endoplasmic reticulum
por: Saric, Amra, et al.
Publicado: (2021)

Image-based analysis of living mammalian cells using label-free 3D refractive index maps reveals new organelle dynamics and dry mass flux
por: Sandoz, Patrick A., et al.
Publicado: (2019)

High-yield ligation-free assembly of DNA constructs with nucleosome positioning sequence repeats for single-molecule manipulation assays
por: Lin, Yi-Yun, et al.
Publicado: (2023)

Proteomics of a fuzzy organelle: interphase chromatin
por: Kustatscher, Georg, et al.
Publicado: (2014)

Data-driven analyses of motor impairments in animal models of neurological disorders
por: Ryait, Hardeep, et al.
Publicado: (2019)

Inducible manipulation of motor–cargo interaction using engineered kinesin motors
por: Hummel, Jessica J. A., et al.
Publicado: (2021)

A Comprehensive Comparison of Transmembrane Domains Reveals Organelle-Specific Properties
por: Sharpe, Hayley J., et al.
Publicado: (2010)

BigBrain 3D atlas of cortical layers: Cortical and laminar thickness gradients diverge in sensory and motor cortices
por: Wagstyl, Konrad, et al.
Publicado: (2020)

Mechanisms of Polarized Organelle Distribution in Neurons
por: Britt, Dylan J., et al.
Publicado: (2016)

Distributed biotin–streptavidin transcription roadblocks for mapping cotranscriptional RNA folding
por: Strobel, Eric J., et al.
Publicado: (2017)

Purification of synchronized Escherichia coli transcription elongation complexes by reversible immobilization on magnetic beads
por: Kelly, Skyler L., et al.
Publicado: (2022)

PE-STOP: A versatile tool for installing nonsense substitutions amenable for precise reversion
por: Song, Ziguo, et al.
Publicado: (2023)

Multiplexed long-read plasmid validation and analysis using OnRamp
por: Mumm, Camille, et al.
Publicado: (2023)

A plasmid DNA-launched SARS-CoV-2 reverse genetics system and coronavirus toolkit for COVID-19 research
por: Rihn, Suzannah J., et al.
Publicado: (2021)

Multiple marker abundance profiling: combining selected reaction monitoring and data‐dependent acquisition for rapid estimation of organelle abundance in subcellular samples
por: Hooper, Cornelia M., et al.
Publicado: (2017)

An artificial processivity clamp made with streptavidin facilitates oriented attachment of polymerase–DNA complexes to surfaces
por: Williams, John G. K., et al.
Publicado: (2008)

An optimized streptavidin-binding RNA aptamer for purification of ribonucleoprotein complexes identifies novel ARE-binding proteins
por: Leppek, Kathrin, et al.
Publicado: (2014)

Manipulation of KLF4 Expression Generates iPSCs Paused at Successive Stages of Reprogramming
por: Nishimura, Ken, et al.
Publicado: (2014)

Compartmentalization of HP1 Proteins in Pluripotency Acquisition and Maintenance
por: Zaidan, Nur Zafirah, et al.
Publicado: (2018)

An Adeno-Associated Virus-Based Toolkit for Preferential Targeting and Manipulating Quiescent Neural Stem Cells in the Adult Hippocampus
por: Crowther, Andrew J., et al.
Publicado: (2018)

Exposure of iPSC-derived human microglia to brain substrates enables the generation and manipulation of diverse transcriptional states in vitro
por: Dolan, Michael-John, et al.
Publicado: (2023)

Prediction of drug cocktail effects when the number of measurements is limited
por: Zimmer, Anat, et al.
Publicado: (2017)

An atlas of Caenorhabditis elegans chemoreceptor expression
por: Vidal, Berta, et al.
Publicado: (2018)

Evaluation of e-liquid toxicity using an open-source high-throughput screening assay
por: Sassano, M. Flori, et al.
Publicado: (2018)

The Plasmodium falciparum transcriptome in severe malaria reveals altered expression of genes involved in important processes including surface antigen–encoding var genes
por: Tonkin-Hill, Gerry Q., et al.
Publicado: (2018)

Evaluation of RNAi and CRISPR technologies by large-scale gene expression profiling in the Connectivity Map
por: Smith, Ian, et al.
Publicado: (2017)

The sea cucumber genome provides insights into morphological evolution and visceral regeneration
por: Zhang, Xiaojun, et al.
Publicado: (2017)

Reporters to mark and eliminate basal or luminal epithelial cells in culture and in vivo
por: Sonzogni, Olmo, et al.
Publicado: (2018)

Classes and continua of hippocampal CA1 inhibitory neurons revealed by single-cell transcriptomics
por: Harris, Kenneth D., et al.
Publicado: (2018)

CellProfiler 3.0: Next-generation image processing for biology
por: McQuin, Claire, et al.
Publicado: (2018)

Decoding the chromatin proteome of a single genomic locus by DNA sequencing
por: Korthout, Tessy, et al.
Publicado: (2018)

PROMIS, global analysis of PROtein–metabolite interactions using size separation in Arabidopsis thaliana
por: Veyel, Daniel, et al.
Publicado: (2018)

Long-term all-optical interrogation of cortical neurons in awake-behaving nonhuman primates
por: Ju, Niansheng, et al.
Publicado: (2018)

De novo assembly of a young Drosophila Y chromosome using single-molecule sequencing and chromatin conformation capture
por: Mahajan, Shivani, et al.
Publicado: (2018)

A systems genetics resource and analysis of sleep regulation in the mouse
por: Diessler, Shanaz, et al.
Publicado: (2018)

Transcriptional programming of lipid and amino acid metabolism by the skeletal muscle circadian clock
por: Dyar, Kenneth Allen, et al.
Publicado: (2018)

Insight into small molecule binding to the neonatal Fc receptor by X-ray crystallography and 100 kHz magic-angle-spinning NMR
por: Stöppler, Daniel, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_d072ddrbclihjht2nj1rfl2siq