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Exploration of antibiotic resistance risks in a veterinary teaching hospital with Oxford Nanopore long read sequencing
The Oxford Nanopore MinION DNA sequencing device can produce large amounts of long sequences, typically several kilobases, within a few hours. This long read capacity was exploited to detect antimicrobial resistance genes (ARGs) in a large veterinary teaching hospital environment, and to assess thei...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542553/ https://www.ncbi.nlm.nih.gov/pubmed/31145757 http://dx.doi.org/10.1371/journal.pone.0217600 |
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author | Kamathewatta, Kanishka Indiwari Bushell, Rhys Nathan Young, Neil David Stevenson, Mark Anthony Billman-Jacobe, Helen Browning, Glenn Francis Marenda, Marc Serge |
author_facet | Kamathewatta, Kanishka Indiwari Bushell, Rhys Nathan Young, Neil David Stevenson, Mark Anthony Billman-Jacobe, Helen Browning, Glenn Francis Marenda, Marc Serge |
author_sort | Kamathewatta, Kanishka Indiwari |
collection | PubMed |
description | The Oxford Nanopore MinION DNA sequencing device can produce large amounts of long sequences, typically several kilobases, within a few hours. This long read capacity was exploited to detect antimicrobial resistance genes (ARGs) in a large veterinary teaching hospital environment, and to assess their taxonomic origin, genetic organisation and association with mobilisation markers concurrently. Samples were collected on eight occasions between November 2016 and May 2017 (inclusive) in a longitudinal study. Nanopore sequencing was performed on total DNA extracted from the samples after a minimal enrichment step in broth. Many ARGs present in the veterinary hospital environment could potentially confer resistance to antimicrobials widely used in treating infections of companion animals, including aminoglycosides, extended-spectrum beta-lactams, sulphonamides, macrolides, and tetracyclines. High-risk ARGs, defined here as single or multiple ARGs associated with pathogenic bacterial species or with mobile genetic elements, were shared between the intensive care unit (ICU) patient cages, a dedicated laundry trolley and a floor cleaning mop-bucket. By contrast, a floor surface from an office corridor without animal contact and located outside the veterinary hospital did not contain such high-risk ARGs. Relative abundances of high-risk ARGs and co-localisation of these genes on the same sequence read were higher in the laundry trolley and mop bucket samples, compared to the ICU cages, suggesting that amplification of ARGs is likely to occur in the collection points for hospital waste. These findings have prompted the implementation of targeted intervention measures in the veterinary hospital to mitigate the risks of transferring clinically important ARGs between sites and to improve biosecurity practices in the facility. |
format | Online Article Text |
id | pubmed-6542553 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-65425532019-06-17 Exploration of antibiotic resistance risks in a veterinary teaching hospital with Oxford Nanopore long read sequencing Kamathewatta, Kanishka Indiwari Bushell, Rhys Nathan Young, Neil David Stevenson, Mark Anthony Billman-Jacobe, Helen Browning, Glenn Francis Marenda, Marc Serge PLoS One Research Article The Oxford Nanopore MinION DNA sequencing device can produce large amounts of long sequences, typically several kilobases, within a few hours. This long read capacity was exploited to detect antimicrobial resistance genes (ARGs) in a large veterinary teaching hospital environment, and to assess their taxonomic origin, genetic organisation and association with mobilisation markers concurrently. Samples were collected on eight occasions between November 2016 and May 2017 (inclusive) in a longitudinal study. Nanopore sequencing was performed on total DNA extracted from the samples after a minimal enrichment step in broth. Many ARGs present in the veterinary hospital environment could potentially confer resistance to antimicrobials widely used in treating infections of companion animals, including aminoglycosides, extended-spectrum beta-lactams, sulphonamides, macrolides, and tetracyclines. High-risk ARGs, defined here as single or multiple ARGs associated with pathogenic bacterial species or with mobile genetic elements, were shared between the intensive care unit (ICU) patient cages, a dedicated laundry trolley and a floor cleaning mop-bucket. By contrast, a floor surface from an office corridor without animal contact and located outside the veterinary hospital did not contain such high-risk ARGs. Relative abundances of high-risk ARGs and co-localisation of these genes on the same sequence read were higher in the laundry trolley and mop bucket samples, compared to the ICU cages, suggesting that amplification of ARGs is likely to occur in the collection points for hospital waste. These findings have prompted the implementation of targeted intervention measures in the veterinary hospital to mitigate the risks of transferring clinically important ARGs between sites and to improve biosecurity practices in the facility. Public Library of Science 2019-05-30 /pmc/articles/PMC6542553/ /pubmed/31145757 http://dx.doi.org/10.1371/journal.pone.0217600 Text en © 2019 Kamathewatta et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kamathewatta, Kanishka Indiwari Bushell, Rhys Nathan Young, Neil David Stevenson, Mark Anthony Billman-Jacobe, Helen Browning, Glenn Francis Marenda, Marc Serge Exploration of antibiotic resistance risks in a veterinary teaching hospital with Oxford Nanopore long read sequencing |
title | Exploration of antibiotic resistance risks in a veterinary teaching hospital with Oxford Nanopore long read sequencing |
title_full | Exploration of antibiotic resistance risks in a veterinary teaching hospital with Oxford Nanopore long read sequencing |
title_fullStr | Exploration of antibiotic resistance risks in a veterinary teaching hospital with Oxford Nanopore long read sequencing |
title_full_unstemmed | Exploration of antibiotic resistance risks in a veterinary teaching hospital with Oxford Nanopore long read sequencing |
title_short | Exploration of antibiotic resistance risks in a veterinary teaching hospital with Oxford Nanopore long read sequencing |
title_sort | exploration of antibiotic resistance risks in a veterinary teaching hospital with oxford nanopore long read sequencing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542553/ https://www.ncbi.nlm.nih.gov/pubmed/31145757 http://dx.doi.org/10.1371/journal.pone.0217600 |
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