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Reciprocal action of Casein Kinase Iε on core planar polarity proteins regulates clustering and asymmetric localisation

The conserved core planar polarity pathway is essential for coordinating polarised cell behaviours and the formation of polarised structures such as cilia and hairs. Core planar polarity proteins localise asymmetrically to opposite cell ends and form intercellular complexes that link the polarity of...

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Autores principales: Strutt, Helen, Gamage, Jessica, Strutt, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542583/
https://www.ncbi.nlm.nih.gov/pubmed/31090542
http://dx.doi.org/10.7554/eLife.45107
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author Strutt, Helen
Gamage, Jessica
Strutt, David
author_facet Strutt, Helen
Gamage, Jessica
Strutt, David
author_sort Strutt, Helen
collection PubMed
description The conserved core planar polarity pathway is essential for coordinating polarised cell behaviours and the formation of polarised structures such as cilia and hairs. Core planar polarity proteins localise asymmetrically to opposite cell ends and form intercellular complexes that link the polarity of neighbouring cells. This asymmetric segregation is regulated by phosphorylation through poorly understood mechanisms. We show that loss of phosphorylation of the core protein Strabismus in the Drosophila pupal wing increases its stability and promotes its clustering at intercellular junctions, and that Prickle negatively regulates Strabismus phosphorylation. Additionally, loss of phosphorylation of Dishevelled – which normally localises to opposite cell edges to Strabismus – reduces its stability at junctions. Moreover, both phosphorylation events are independently mediated by Casein Kinase Iε. We conclude that Casein Kinase Iε phosphorylation acts as a switch, promoting Strabismus mobility and Dishevelled immobility, thus enhancing sorting of these proteins to opposite cell edges.
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spelling pubmed-65425832019-06-12 Reciprocal action of Casein Kinase Iε on core planar polarity proteins regulates clustering and asymmetric localisation Strutt, Helen Gamage, Jessica Strutt, David eLife Developmental Biology The conserved core planar polarity pathway is essential for coordinating polarised cell behaviours and the formation of polarised structures such as cilia and hairs. Core planar polarity proteins localise asymmetrically to opposite cell ends and form intercellular complexes that link the polarity of neighbouring cells. This asymmetric segregation is regulated by phosphorylation through poorly understood mechanisms. We show that loss of phosphorylation of the core protein Strabismus in the Drosophila pupal wing increases its stability and promotes its clustering at intercellular junctions, and that Prickle negatively regulates Strabismus phosphorylation. Additionally, loss of phosphorylation of Dishevelled – which normally localises to opposite cell edges to Strabismus – reduces its stability at junctions. Moreover, both phosphorylation events are independently mediated by Casein Kinase Iε. We conclude that Casein Kinase Iε phosphorylation acts as a switch, promoting Strabismus mobility and Dishevelled immobility, thus enhancing sorting of these proteins to opposite cell edges. eLife Sciences Publications, Ltd 2019-05-15 /pmc/articles/PMC6542583/ /pubmed/31090542 http://dx.doi.org/10.7554/eLife.45107 Text en © 2019, Strutt et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Strutt, Helen
Gamage, Jessica
Strutt, David
Reciprocal action of Casein Kinase Iε on core planar polarity proteins regulates clustering and asymmetric localisation
title Reciprocal action of Casein Kinase Iε on core planar polarity proteins regulates clustering and asymmetric localisation
title_full Reciprocal action of Casein Kinase Iε on core planar polarity proteins regulates clustering and asymmetric localisation
title_fullStr Reciprocal action of Casein Kinase Iε on core planar polarity proteins regulates clustering and asymmetric localisation
title_full_unstemmed Reciprocal action of Casein Kinase Iε on core planar polarity proteins regulates clustering and asymmetric localisation
title_short Reciprocal action of Casein Kinase Iε on core planar polarity proteins regulates clustering and asymmetric localisation
title_sort reciprocal action of casein kinase iε on core planar polarity proteins regulates clustering and asymmetric localisation
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542583/
https://www.ncbi.nlm.nih.gov/pubmed/31090542
http://dx.doi.org/10.7554/eLife.45107
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