Improved matrix coating for positive- and negative-ion-mode MALDI-TOF imaging of lipids in blood vessel tissues

High-quality matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) of lipids in biological tissue relies on the fabrication of a homogeneous matrix coating featuring best possible analyte integration. This communication addresses a matrix vapor deposition/recrystallizatio...

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Detalles Bibliográficos
Autores principales: Meisenbichler, Christina, Doppler, Christian, Bernhard, David, Müller, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542778/
https://www.ncbi.nlm.nih.gov/pubmed/31037373
http://dx.doi.org/10.1007/s00216-019-01826-x
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author Meisenbichler, Christina
Doppler, Christian
Bernhard, David
Müller, Thomas
author_facet Meisenbichler, Christina
Doppler, Christian
Bernhard, David
Müller, Thomas
author_sort Meisenbichler, Christina
collection PubMed
description High-quality matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) of lipids in biological tissue relies on the fabrication of a homogeneous matrix coating featuring best possible analyte integration. This communication addresses a matrix vapor deposition/recrystallization process for the application of 1,5-diaminonaphthalene (1,5-DAN) onto slices of human aortic tissue. The matrix coating is compatible with both positive- as well as negative-ion-mode MALDI MSI facilitating a significantly enhanced detection of lipid-related signals in different cell layers of blood vessel walls. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00216-019-01826-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-65427782019-06-14 Improved matrix coating for positive- and negative-ion-mode MALDI-TOF imaging of lipids in blood vessel tissues Meisenbichler, Christina Doppler, Christian Bernhard, David Müller, Thomas Anal Bioanal Chem Communication High-quality matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) of lipids in biological tissue relies on the fabrication of a homogeneous matrix coating featuring best possible analyte integration. This communication addresses a matrix vapor deposition/recrystallization process for the application of 1,5-diaminonaphthalene (1,5-DAN) onto slices of human aortic tissue. The matrix coating is compatible with both positive- as well as negative-ion-mode MALDI MSI facilitating a significantly enhanced detection of lipid-related signals in different cell layers of blood vessel walls. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00216-019-01826-x) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2019-04-30 2019 /pmc/articles/PMC6542778/ /pubmed/31037373 http://dx.doi.org/10.1007/s00216-019-01826-x Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Communication
Meisenbichler, Christina
Doppler, Christian
Bernhard, David
Müller, Thomas
Improved matrix coating for positive- and negative-ion-mode MALDI-TOF imaging of lipids in blood vessel tissues
title Improved matrix coating for positive- and negative-ion-mode MALDI-TOF imaging of lipids in blood vessel tissues
title_full Improved matrix coating for positive- and negative-ion-mode MALDI-TOF imaging of lipids in blood vessel tissues
title_fullStr Improved matrix coating for positive- and negative-ion-mode MALDI-TOF imaging of lipids in blood vessel tissues
title_full_unstemmed Improved matrix coating for positive- and negative-ion-mode MALDI-TOF imaging of lipids in blood vessel tissues
title_short Improved matrix coating for positive- and negative-ion-mode MALDI-TOF imaging of lipids in blood vessel tissues
title_sort improved matrix coating for positive- and negative-ion-mode maldi-tof imaging of lipids in blood vessel tissues
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542778/
https://www.ncbi.nlm.nih.gov/pubmed/31037373
http://dx.doi.org/10.1007/s00216-019-01826-x
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AT bernharddavid improvedmatrixcoatingforpositiveandnegativeionmodemalditofimagingoflipidsinbloodvesseltissues
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