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Evaluation of Molecularly Imprinted Polymers as Synthetic Virus Neutralizing Antibody Mimics
Rapid development of antibody-based therapeutics are crucial to the agenda of innovative manufacturing of macromolecular therapies to combat emergent diseases. Although highly specific, antibody therapies are costly to produce. Molecularly imprinted polymers (MIPs) constitute a rapidly-evolving clas...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542949/ https://www.ncbi.nlm.nih.gov/pubmed/31179277 http://dx.doi.org/10.3389/fbioe.2019.00115 |
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author | Graham, Simon P. El-Sharif, Hazim F. Hussain, Sabha Fruengel, Rieke McLean, Rebecca K. Hawes, Philippa C. Sullivan, Mark V. Reddy, Subrayal M. |
author_facet | Graham, Simon P. El-Sharif, Hazim F. Hussain, Sabha Fruengel, Rieke McLean, Rebecca K. Hawes, Philippa C. Sullivan, Mark V. Reddy, Subrayal M. |
author_sort | Graham, Simon P. |
collection | PubMed |
description | Rapid development of antibody-based therapeutics are crucial to the agenda of innovative manufacturing of macromolecular therapies to combat emergent diseases. Although highly specific, antibody therapies are costly to produce. Molecularly imprinted polymers (MIPs) constitute a rapidly-evolving class of antigen-recognition materials that act as synthetic antibodies. We report here on the virus neutralizing capacity of hydrogel-based MIPs. We produced MIPs using porcine reproductive and respiratory syndrome virus (PRRSV-1), as a model mammalian virus. Assays were performed to evaluate the specificity of virus neutralization, the effect of incubation time and MIP concentration. Polyacrylamide and N-hydroxymethylacrylamide based MIPs produced a highly significant reduction in infectious viral titer recovered after treatment, reducing it to the limit of detection of the assay. MIP specificity was tested by comparing their neutralizing effects on PRRSV-1 to the effects on the unrelated bovine viral diarrhea virus-1; no significant cross-reactivity was observed. The MIPs demonstrated effective virus neutralization in just 2.5 min and their effect was concentration dependent. These data support the further evaluation of MIPs as synthetic antibodies as a novel approach to the treatment of viral infection. |
format | Online Article Text |
id | pubmed-6542949 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65429492019-06-07 Evaluation of Molecularly Imprinted Polymers as Synthetic Virus Neutralizing Antibody Mimics Graham, Simon P. El-Sharif, Hazim F. Hussain, Sabha Fruengel, Rieke McLean, Rebecca K. Hawes, Philippa C. Sullivan, Mark V. Reddy, Subrayal M. Front Bioeng Biotechnol Bioengineering and Biotechnology Rapid development of antibody-based therapeutics are crucial to the agenda of innovative manufacturing of macromolecular therapies to combat emergent diseases. Although highly specific, antibody therapies are costly to produce. Molecularly imprinted polymers (MIPs) constitute a rapidly-evolving class of antigen-recognition materials that act as synthetic antibodies. We report here on the virus neutralizing capacity of hydrogel-based MIPs. We produced MIPs using porcine reproductive and respiratory syndrome virus (PRRSV-1), as a model mammalian virus. Assays were performed to evaluate the specificity of virus neutralization, the effect of incubation time and MIP concentration. Polyacrylamide and N-hydroxymethylacrylamide based MIPs produced a highly significant reduction in infectious viral titer recovered after treatment, reducing it to the limit of detection of the assay. MIP specificity was tested by comparing their neutralizing effects on PRRSV-1 to the effects on the unrelated bovine viral diarrhea virus-1; no significant cross-reactivity was observed. The MIPs demonstrated effective virus neutralization in just 2.5 min and their effect was concentration dependent. These data support the further evaluation of MIPs as synthetic antibodies as a novel approach to the treatment of viral infection. Frontiers Media S.A. 2019-05-24 /pmc/articles/PMC6542949/ /pubmed/31179277 http://dx.doi.org/10.3389/fbioe.2019.00115 Text en Copyright © 2019 Graham, El-Sharif, Hussain, Fruengel, McLean, Hawes, Sullivan and Reddy. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Graham, Simon P. El-Sharif, Hazim F. Hussain, Sabha Fruengel, Rieke McLean, Rebecca K. Hawes, Philippa C. Sullivan, Mark V. Reddy, Subrayal M. Evaluation of Molecularly Imprinted Polymers as Synthetic Virus Neutralizing Antibody Mimics |
title | Evaluation of Molecularly Imprinted Polymers as Synthetic Virus Neutralizing Antibody Mimics |
title_full | Evaluation of Molecularly Imprinted Polymers as Synthetic Virus Neutralizing Antibody Mimics |
title_fullStr | Evaluation of Molecularly Imprinted Polymers as Synthetic Virus Neutralizing Antibody Mimics |
title_full_unstemmed | Evaluation of Molecularly Imprinted Polymers as Synthetic Virus Neutralizing Antibody Mimics |
title_short | Evaluation of Molecularly Imprinted Polymers as Synthetic Virus Neutralizing Antibody Mimics |
title_sort | evaluation of molecularly imprinted polymers as synthetic virus neutralizing antibody mimics |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542949/ https://www.ncbi.nlm.nih.gov/pubmed/31179277 http://dx.doi.org/10.3389/fbioe.2019.00115 |
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