A novel anti Candida albicans drug screening system based on high-throughput microfluidic chips

Due to the antibacterial resistance crisis, developing new antibacterials is of particular interest. In this study, we combined the antifungal drug amphotericin B with 50,520 different small molecule compounds obtained from the Chinese National Compound Library in an attempt to improve its efficacy...

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Autores principales: Qiang, Le, Guo, Jing, Han, Yingkuan, Jiang, Jianfeng, Su, Xiaowen, Liu, Hong, Qi, Qingguo, Han, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6543036/
https://www.ncbi.nlm.nih.gov/pubmed/31147583
http://dx.doi.org/10.1038/s41598-019-44298-w
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author Qiang, Le
Guo, Jing
Han, Yingkuan
Jiang, Jianfeng
Su, Xiaowen
Liu, Hong
Qi, Qingguo
Han, Lin
author_facet Qiang, Le
Guo, Jing
Han, Yingkuan
Jiang, Jianfeng
Su, Xiaowen
Liu, Hong
Qi, Qingguo
Han, Lin
author_sort Qiang, Le
collection PubMed
description Due to the antibacterial resistance crisis, developing new antibacterials is of particular interest. In this study, we combined the antifungal drug amphotericin B with 50,520 different small molecule compounds obtained from the Chinese National Compound Library in an attempt to improve its efficacy against Candida albicans persister cells. To systematically study the antifungal effect of each compound, we utilized custom-designed high-throughput microfluidic chips. Our microfluidic chips contained microchannels ranging from 3 µm to 5 µm in width to allow Candida albicans cells to line up one-by-one to facilitate fluorescence-microscope viewing. After screening, we were left with 10 small molecule compounds that improved the antifungal effects of amphotericin B more than 30% against Candida albicans persister cells.
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spelling pubmed-65430362019-06-07 A novel anti Candida albicans drug screening system based on high-throughput microfluidic chips Qiang, Le Guo, Jing Han, Yingkuan Jiang, Jianfeng Su, Xiaowen Liu, Hong Qi, Qingguo Han, Lin Sci Rep Article Due to the antibacterial resistance crisis, developing new antibacterials is of particular interest. In this study, we combined the antifungal drug amphotericin B with 50,520 different small molecule compounds obtained from the Chinese National Compound Library in an attempt to improve its efficacy against Candida albicans persister cells. To systematically study the antifungal effect of each compound, we utilized custom-designed high-throughput microfluidic chips. Our microfluidic chips contained microchannels ranging from 3 µm to 5 µm in width to allow Candida albicans cells to line up one-by-one to facilitate fluorescence-microscope viewing. After screening, we were left with 10 small molecule compounds that improved the antifungal effects of amphotericin B more than 30% against Candida albicans persister cells. Nature Publishing Group UK 2019-05-30 /pmc/articles/PMC6543036/ /pubmed/31147583 http://dx.doi.org/10.1038/s41598-019-44298-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Qiang, Le
Guo, Jing
Han, Yingkuan
Jiang, Jianfeng
Su, Xiaowen
Liu, Hong
Qi, Qingguo
Han, Lin
A novel anti Candida albicans drug screening system based on high-throughput microfluidic chips
title A novel anti Candida albicans drug screening system based on high-throughput microfluidic chips
title_full A novel anti Candida albicans drug screening system based on high-throughput microfluidic chips
title_fullStr A novel anti Candida albicans drug screening system based on high-throughput microfluidic chips
title_full_unstemmed A novel anti Candida albicans drug screening system based on high-throughput microfluidic chips
title_short A novel anti Candida albicans drug screening system based on high-throughput microfluidic chips
title_sort novel anti candida albicans drug screening system based on high-throughput microfluidic chips
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6543036/
https://www.ncbi.nlm.nih.gov/pubmed/31147583
http://dx.doi.org/10.1038/s41598-019-44298-w
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