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Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin
Helmintex is a sensitive method used for detecting Schistosoma mansoni eggs. Here, we describe the observed frequency of six proposed criteria associated with the identification of S. mansoni eggs prepared with the Helmintex method and stained with ninhydrin. The efficacy of these criteria in classi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6543901/ https://www.ncbi.nlm.nih.gov/pubmed/31166420 http://dx.doi.org/10.1590/0074-02760180529 |
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author | de Souza, Renata Perotto Favero, Vivian Pascoal, Vanessa Fey Lindholz, Catieli Bittencourt, Hélio Radke Graeff-Teixeira, Carlos |
author_facet | de Souza, Renata Perotto Favero, Vivian Pascoal, Vanessa Fey Lindholz, Catieli Bittencourt, Hélio Radke Graeff-Teixeira, Carlos |
author_sort | de Souza, Renata Perotto |
collection | PubMed |
description | Helmintex is a sensitive method used for detecting Schistosoma mansoni eggs. Here, we describe the observed frequency of six proposed criteria associated with the identification of S. mansoni eggs prepared with the Helmintex method and stained with ninhydrin. The efficacy of these criteria in classifying S. mansoni eggs when applied in various combinations was also examined. Nine observers registered the presence or absence of 6 different criteria in 100 eggs using a microscope at 100x magnification. Ninhydrin purple, which was frequently observed, was the criterion associated with the lowest inter-observer variability. At least three criteria were associated with a significantly better performance in egg identification. In conclusion, ninhydrin staining and a combination of criteria are recommended for microscope examination of faecal sediments. |
format | Online Article Text |
id | pubmed-6543901 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-65439012019-06-12 Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin de Souza, Renata Perotto Favero, Vivian Pascoal, Vanessa Fey Lindholz, Catieli Bittencourt, Hélio Radke Graeff-Teixeira, Carlos Mem Inst Oswaldo Cruz Short Communication Helmintex is a sensitive method used for detecting Schistosoma mansoni eggs. Here, we describe the observed frequency of six proposed criteria associated with the identification of S. mansoni eggs prepared with the Helmintex method and stained with ninhydrin. The efficacy of these criteria in classifying S. mansoni eggs when applied in various combinations was also examined. Nine observers registered the presence or absence of 6 different criteria in 100 eggs using a microscope at 100x magnification. Ninhydrin purple, which was frequently observed, was the criterion associated with the lowest inter-observer variability. At least three criteria were associated with a significantly better performance in egg identification. In conclusion, ninhydrin staining and a combination of criteria are recommended for microscope examination of faecal sediments. Instituto Oswaldo Cruz, Ministério da Saúde 2019-05-30 /pmc/articles/PMC6543901/ /pubmed/31166420 http://dx.doi.org/10.1590/0074-02760180529 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Short Communication de Souza, Renata Perotto Favero, Vivian Pascoal, Vanessa Fey Lindholz, Catieli Bittencourt, Hélio Radke Graeff-Teixeira, Carlos Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin |
title | Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin |
title_full | Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin |
title_fullStr | Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin |
title_full_unstemmed | Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin |
title_short | Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin |
title_sort | criteria for identification of schistosoma mansoni eggs in faecal sediments prepared with the helmintex method and stained by ninhydrin |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6543901/ https://www.ncbi.nlm.nih.gov/pubmed/31166420 http://dx.doi.org/10.1590/0074-02760180529 |
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