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Porcine model for the study of liver regeneration enhanced by non-invasive (13)C-methacetin breath test (LiMAx test) and permanent portal venous access

INTRODUCTION: Despite advances in perioperative management and surgical technique, postoperative liver failure remains a feared complication after hepatic resection. Various supportive treatment options are under current discussion, but lack of structured evaluation. We therefore established a porci...

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Autores principales: Wittauer, Eva-Maria, Oldhafer, Felix, Augstein, Eva, Beetz, Oliver, Kleine, Moritz, Schumacher, Carsten, Sieg, Lion, Eismann, Hendrik, Johanning, Kai, Bleich, André, Vondran, Florian Wolfgang Rudolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544243/
https://www.ncbi.nlm.nih.gov/pubmed/31150446
http://dx.doi.org/10.1371/journal.pone.0217488
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author Wittauer, Eva-Maria
Oldhafer, Felix
Augstein, Eva
Beetz, Oliver
Kleine, Moritz
Schumacher, Carsten
Sieg, Lion
Eismann, Hendrik
Johanning, Kai
Bleich, André
Vondran, Florian Wolfgang Rudolf
author_facet Wittauer, Eva-Maria
Oldhafer, Felix
Augstein, Eva
Beetz, Oliver
Kleine, Moritz
Schumacher, Carsten
Sieg, Lion
Eismann, Hendrik
Johanning, Kai
Bleich, André
Vondran, Florian Wolfgang Rudolf
author_sort Wittauer, Eva-Maria
collection PubMed
description INTRODUCTION: Despite advances in perioperative management and surgical technique, postoperative liver failure remains a feared complication after hepatic resection. Various supportive treatment options are under current discussion, but lack of structured evaluation. We therefore established a porcine model of major liver resection to study regeneration after partial hepatectomy in a reliable and well-defined pre-clinical setting. METHODS: Major hepatectomy was performed on seven minipigs with the intention to set up a non-lethal but relevant transient impairment of liver function. For steady postoperative vascular access (e.g. for blood withdrawal, measurement of venous pressure), permanent catheters were implanted into the internal jugular and portal veins, respectively. Animals were followed up for 30 days; clinical and laboratory results were recorded in detail. Monitoring was enhanced by non-invasive determination of the maximum liver function capacity (LiMAx test). RESULTS AND CONCLUSIONS: The established porcine model appeared suitable for evaluation of postoperative liver regeneration. Clinical characteristics and progression of liver function impairment as well as subsequent recovery were comparable to courses known from surgery in humans. Laboratory parameters (e.g. liver enzymes, bilirubin, INR, coagulation factor II) showed relevant derangements during postoperative days (POD) 0 to 3 followed by normalization until POD 7. Application of the LiMAx test was feasible in minipigs, again showing values comparable to humans and kinetics in line with obtained laboratory parameters. The exteriorized portal vein catheters enabled intra- and postoperative monitoring of portal venous pressures as well as easy access for blood withdrawal without relevant risk of postoperative complications.
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spelling pubmed-65442432019-06-17 Porcine model for the study of liver regeneration enhanced by non-invasive (13)C-methacetin breath test (LiMAx test) and permanent portal venous access Wittauer, Eva-Maria Oldhafer, Felix Augstein, Eva Beetz, Oliver Kleine, Moritz Schumacher, Carsten Sieg, Lion Eismann, Hendrik Johanning, Kai Bleich, André Vondran, Florian Wolfgang Rudolf PLoS One Research Article INTRODUCTION: Despite advances in perioperative management and surgical technique, postoperative liver failure remains a feared complication after hepatic resection. Various supportive treatment options are under current discussion, but lack of structured evaluation. We therefore established a porcine model of major liver resection to study regeneration after partial hepatectomy in a reliable and well-defined pre-clinical setting. METHODS: Major hepatectomy was performed on seven minipigs with the intention to set up a non-lethal but relevant transient impairment of liver function. For steady postoperative vascular access (e.g. for blood withdrawal, measurement of venous pressure), permanent catheters were implanted into the internal jugular and portal veins, respectively. Animals were followed up for 30 days; clinical and laboratory results were recorded in detail. Monitoring was enhanced by non-invasive determination of the maximum liver function capacity (LiMAx test). RESULTS AND CONCLUSIONS: The established porcine model appeared suitable for evaluation of postoperative liver regeneration. Clinical characteristics and progression of liver function impairment as well as subsequent recovery were comparable to courses known from surgery in humans. Laboratory parameters (e.g. liver enzymes, bilirubin, INR, coagulation factor II) showed relevant derangements during postoperative days (POD) 0 to 3 followed by normalization until POD 7. Application of the LiMAx test was feasible in minipigs, again showing values comparable to humans and kinetics in line with obtained laboratory parameters. The exteriorized portal vein catheters enabled intra- and postoperative monitoring of portal venous pressures as well as easy access for blood withdrawal without relevant risk of postoperative complications. Public Library of Science 2019-05-31 /pmc/articles/PMC6544243/ /pubmed/31150446 http://dx.doi.org/10.1371/journal.pone.0217488 Text en © 2019 Wittauer et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wittauer, Eva-Maria
Oldhafer, Felix
Augstein, Eva
Beetz, Oliver
Kleine, Moritz
Schumacher, Carsten
Sieg, Lion
Eismann, Hendrik
Johanning, Kai
Bleich, André
Vondran, Florian Wolfgang Rudolf
Porcine model for the study of liver regeneration enhanced by non-invasive (13)C-methacetin breath test (LiMAx test) and permanent portal venous access
title Porcine model for the study of liver regeneration enhanced by non-invasive (13)C-methacetin breath test (LiMAx test) and permanent portal venous access
title_full Porcine model for the study of liver regeneration enhanced by non-invasive (13)C-methacetin breath test (LiMAx test) and permanent portal venous access
title_fullStr Porcine model for the study of liver regeneration enhanced by non-invasive (13)C-methacetin breath test (LiMAx test) and permanent portal venous access
title_full_unstemmed Porcine model for the study of liver regeneration enhanced by non-invasive (13)C-methacetin breath test (LiMAx test) and permanent portal venous access
title_short Porcine model for the study of liver regeneration enhanced by non-invasive (13)C-methacetin breath test (LiMAx test) and permanent portal venous access
title_sort porcine model for the study of liver regeneration enhanced by non-invasive (13)c-methacetin breath test (limax test) and permanent portal venous access
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544243/
https://www.ncbi.nlm.nih.gov/pubmed/31150446
http://dx.doi.org/10.1371/journal.pone.0217488
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