Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model

A scalably manufacturable oral antibody technology that can interfere with gastrointestinal (GI) targets is needed. Contrary to the complex native secretory IgA, we achieve this using a single-gene encoded monomeric-IgA-like antibody, composed of camelid VHH fused to IgA Fc (mVHH-IgA). This can be p...

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Detalles Bibliográficos
Autores principales: Virdi, Vikram, Palaci, Jorge, Laukens, Bram, Ryckaert, Stefan, Cox, Eric, Vanderbeke, Erik, Depicker, Ann, Callewaert, Nico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544532/
https://www.ncbi.nlm.nih.gov/pubmed/30936561
http://dx.doi.org/10.1038/s41587-019-0070-x
Descripción
Sumario:A scalably manufacturable oral antibody technology that can interfere with gastrointestinal (GI) targets is needed. Contrary to the complex native secretory IgA, we achieve this using a single-gene encoded monomeric-IgA-like antibody, composed of camelid VHH fused to IgA Fc (mVHH-IgA). This can be produced in soybean seeds or secreted from Pichia pastoris yeast, freeze-or spray-dried, and when delivered in food prevents enterotoxigenic Escherichia coli (F4-ETEC) infection in piglets.

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