Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model

A scalably manufacturable oral antibody technology that can interfere with gastrointestinal (GI) targets is needed. Contrary to the complex native secretory IgA, we achieve this using a single-gene encoded monomeric-IgA-like antibody, composed of camelid VHH fused to IgA Fc (mVHH-IgA). This can be p...

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Autores principales: Virdi, Vikram, Palaci, Jorge, Laukens, Bram, Ryckaert, Stefan, Cox, Eric, Vanderbeke, Erik, Depicker, Ann, Callewaert, Nico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544532/
https://www.ncbi.nlm.nih.gov/pubmed/30936561
http://dx.doi.org/10.1038/s41587-019-0070-x
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author Virdi, Vikram
Palaci, Jorge
Laukens, Bram
Ryckaert, Stefan
Cox, Eric
Vanderbeke, Erik
Depicker, Ann
Callewaert, Nico
author_facet Virdi, Vikram
Palaci, Jorge
Laukens, Bram
Ryckaert, Stefan
Cox, Eric
Vanderbeke, Erik
Depicker, Ann
Callewaert, Nico
author_sort Virdi, Vikram
collection PubMed
description A scalably manufacturable oral antibody technology that can interfere with gastrointestinal (GI) targets is needed. Contrary to the complex native secretory IgA, we achieve this using a single-gene encoded monomeric-IgA-like antibody, composed of camelid VHH fused to IgA Fc (mVHH-IgA). This can be produced in soybean seeds or secreted from Pichia pastoris yeast, freeze-or spray-dried, and when delivered in food prevents enterotoxigenic Escherichia coli (F4-ETEC) infection in piglets.
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spelling pubmed-65445322019-10-01 Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model Virdi, Vikram Palaci, Jorge Laukens, Bram Ryckaert, Stefan Cox, Eric Vanderbeke, Erik Depicker, Ann Callewaert, Nico Nat Biotechnol Article A scalably manufacturable oral antibody technology that can interfere with gastrointestinal (GI) targets is needed. Contrary to the complex native secretory IgA, we achieve this using a single-gene encoded monomeric-IgA-like antibody, composed of camelid VHH fused to IgA Fc (mVHH-IgA). This can be produced in soybean seeds or secreted from Pichia pastoris yeast, freeze-or spray-dried, and when delivered in food prevents enterotoxigenic Escherichia coli (F4-ETEC) infection in piglets. 2019-04-01 2019-05 /pmc/articles/PMC6544532/ /pubmed/30936561 http://dx.doi.org/10.1038/s41587-019-0070-x Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Virdi, Vikram
Palaci, Jorge
Laukens, Bram
Ryckaert, Stefan
Cox, Eric
Vanderbeke, Erik
Depicker, Ann
Callewaert, Nico
Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model
title Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model
title_full Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model
title_fullStr Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model
title_full_unstemmed Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model
title_short Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model
title_sort yeast-secreted, dried and food-admixed monomeric iga prevents gastrointestinal infection in a piglet model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544532/
https://www.ncbi.nlm.nih.gov/pubmed/30936561
http://dx.doi.org/10.1038/s41587-019-0070-x
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