Cargando…
Correlation of the temporal and spatial expression patterns of HQT with the biosynthesis and accumulation of chlorogenic acid in Lonicera japonica flowers
Hydroxycinnamoyl-CoA quinate transferase (HQT) is one of the key enzymes in the biosynthesis of chlorogenic acid (CGA) in the flowers of Lonicera japonica. However, the spatiotemporal expression patterns of HQT and its relationship to the dynamics of CGA biosynthesis, transport, and storage remain l...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544646/ https://www.ncbi.nlm.nih.gov/pubmed/31231531 http://dx.doi.org/10.1038/s41438-019-0154-2 |
_version_ | 1783423289669976064 |
---|---|
author | Li, Yanqun Kong, Dexin Bai, Mei He, Hanjun Wang, Haiyang Wu, Hong |
author_facet | Li, Yanqun Kong, Dexin Bai, Mei He, Hanjun Wang, Haiyang Wu, Hong |
author_sort | Li, Yanqun |
collection | PubMed |
description | Hydroxycinnamoyl-CoA quinate transferase (HQT) is one of the key enzymes in the biosynthesis of chlorogenic acid (CGA) in the flowers of Lonicera japonica. However, the spatiotemporal expression patterns of HQT and its relationship to the dynamics of CGA biosynthesis, transport, and storage remain largely unknown. In this study, we collected L. japonica flower samples at different growth stages (S1–S6) and examined the spatiotemporal expression pattern of HQT and the dynamic accumulation patterns of CGA using a combination of molecular and cytological techniques. Our results suggest that the spatiotemporal expression pattern of HQT is directly correlated with dynamic changes in CGA accumulation and distribution in L. japonica flowers. We further show that CGA is synthesized primarily in the cytoplasm and chloroplasts. CGA synthesized in the cytoplasm first accumulates in specialized vesicles and is then transferred to large central vacuoles for storage by fusion of CGA-containing vesicles with vacuoles. Furthermore, CGA synthesized in the chloroplasts appears to be transferred into the vacuoles for storage by direct membrane fusion between the tonoplast and the disrupted chloroplast membranes. Collectively, our results suggest that CGA is synthesized in chloroplasts and cytoplasm and finally transferred to the vacuole for long-term storage. |
format | Online Article Text |
id | pubmed-6544646 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-65446462019-06-21 Correlation of the temporal and spatial expression patterns of HQT with the biosynthesis and accumulation of chlorogenic acid in Lonicera japonica flowers Li, Yanqun Kong, Dexin Bai, Mei He, Hanjun Wang, Haiyang Wu, Hong Hortic Res Article Hydroxycinnamoyl-CoA quinate transferase (HQT) is one of the key enzymes in the biosynthesis of chlorogenic acid (CGA) in the flowers of Lonicera japonica. However, the spatiotemporal expression patterns of HQT and its relationship to the dynamics of CGA biosynthesis, transport, and storage remain largely unknown. In this study, we collected L. japonica flower samples at different growth stages (S1–S6) and examined the spatiotemporal expression pattern of HQT and the dynamic accumulation patterns of CGA using a combination of molecular and cytological techniques. Our results suggest that the spatiotemporal expression pattern of HQT is directly correlated with dynamic changes in CGA accumulation and distribution in L. japonica flowers. We further show that CGA is synthesized primarily in the cytoplasm and chloroplasts. CGA synthesized in the cytoplasm first accumulates in specialized vesicles and is then transferred to large central vacuoles for storage by fusion of CGA-containing vesicles with vacuoles. Furthermore, CGA synthesized in the chloroplasts appears to be transferred into the vacuoles for storage by direct membrane fusion between the tonoplast and the disrupted chloroplast membranes. Collectively, our results suggest that CGA is synthesized in chloroplasts and cytoplasm and finally transferred to the vacuole for long-term storage. Nature Publishing Group UK 2019-06-01 /pmc/articles/PMC6544646/ /pubmed/31231531 http://dx.doi.org/10.1038/s41438-019-0154-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Li, Yanqun Kong, Dexin Bai, Mei He, Hanjun Wang, Haiyang Wu, Hong Correlation of the temporal and spatial expression patterns of HQT with the biosynthesis and accumulation of chlorogenic acid in Lonicera japonica flowers |
title | Correlation of the temporal and spatial expression patterns of HQT with the biosynthesis and accumulation of chlorogenic acid in Lonicera japonica flowers |
title_full | Correlation of the temporal and spatial expression patterns of HQT with the biosynthesis and accumulation of chlorogenic acid in Lonicera japonica flowers |
title_fullStr | Correlation of the temporal and spatial expression patterns of HQT with the biosynthesis and accumulation of chlorogenic acid in Lonicera japonica flowers |
title_full_unstemmed | Correlation of the temporal and spatial expression patterns of HQT with the biosynthesis and accumulation of chlorogenic acid in Lonicera japonica flowers |
title_short | Correlation of the temporal and spatial expression patterns of HQT with the biosynthesis and accumulation of chlorogenic acid in Lonicera japonica flowers |
title_sort | correlation of the temporal and spatial expression patterns of hqt with the biosynthesis and accumulation of chlorogenic acid in lonicera japonica flowers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544646/ https://www.ncbi.nlm.nih.gov/pubmed/31231531 http://dx.doi.org/10.1038/s41438-019-0154-2 |
work_keys_str_mv | AT liyanqun correlationofthetemporalandspatialexpressionpatternsofhqtwiththebiosynthesisandaccumulationofchlorogenicacidinlonicerajaponicaflowers AT kongdexin correlationofthetemporalandspatialexpressionpatternsofhqtwiththebiosynthesisandaccumulationofchlorogenicacidinlonicerajaponicaflowers AT baimei correlationofthetemporalandspatialexpressionpatternsofhqtwiththebiosynthesisandaccumulationofchlorogenicacidinlonicerajaponicaflowers AT hehanjun correlationofthetemporalandspatialexpressionpatternsofhqtwiththebiosynthesisandaccumulationofchlorogenicacidinlonicerajaponicaflowers AT wanghaiyang correlationofthetemporalandspatialexpressionpatternsofhqtwiththebiosynthesisandaccumulationofchlorogenicacidinlonicerajaponicaflowers AT wuhong correlationofthetemporalandspatialexpressionpatternsofhqtwiththebiosynthesisandaccumulationofchlorogenicacidinlonicerajaponicaflowers |