Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system

Understanding the molecular pathogenesis of Clostridioides difficile has relied on the use of ermB-based mutagens in erythromycin-sensitive strains. However, the repeated subcultures required to isolate sensitive variants can lead to the acquisition of ancillary mutations that affect phenotype, incl...

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Detalles Bibliográficos
Autores principales: Ingle, Patrick, Groothuis, Daphne, Rowe, Peter, Huang, He, Cockayne, Alan, Kuehne, Sarah A., Jiang, Weihong, Gu, Yang, Humphreys, Christopher M., Minton, Nigel P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544763/
https://www.ncbi.nlm.nih.gov/pubmed/31148548
http://dx.doi.org/10.1038/s41598-019-44458-y

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