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Increasing sulfate levels show a differential impact on synthetic communities comprising different methanogens and a sulfate reducer

Methane-producing microbial communities are of ecological and biotechnological interest. Syntrophic interactions among sulfate reducers and aceto/hydrogenotrophic and obligate hydrogenotrophic methanogens form a key component of these communities, yet, the impact of these different syntrophic routes...

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Detalles Bibliográficos
Autores principales: Chen, Jing, Wade, Matthew J., Dolfing, Jan, Soyer, Orkun S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544901/
https://www.ncbi.nlm.nih.gov/pubmed/31064258
http://dx.doi.org/10.1098/rsif.2019.0129
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author Chen, Jing
Wade, Matthew J.
Dolfing, Jan
Soyer, Orkun S.
author_facet Chen, Jing
Wade, Matthew J.
Dolfing, Jan
Soyer, Orkun S.
author_sort Chen, Jing
collection PubMed
description Methane-producing microbial communities are of ecological and biotechnological interest. Syntrophic interactions among sulfate reducers and aceto/hydrogenotrophic and obligate hydrogenotrophic methanogens form a key component of these communities, yet, the impact of these different syntrophic routes on methane production and their stability against sulfate availability are not well understood. Here, we construct model synthetic communities using a sulfate reducer and two types of methanogens representing different methanogenesis routes. We find that tri-cultures with both routes increase methane production by almost twofold compared to co-cultures and are stable in the absence of sulfate. With increasing sulfate, system stability and productivity decreases and does so faster in communities with aceto/hydrogenotrophic methanogens despite the continued presence of acetate. We show that this is due to a shift in the metabolism of these methanogens towards co-utilization of hydrogen with acetate. These findings indicate the important role of hydrogen dynamics in the stability and productivity of syntrophic communities.
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spelling pubmed-65449012019-06-12 Increasing sulfate levels show a differential impact on synthetic communities comprising different methanogens and a sulfate reducer Chen, Jing Wade, Matthew J. Dolfing, Jan Soyer, Orkun S. J R Soc Interface Life Sciences–Earth Science interface Methane-producing microbial communities are of ecological and biotechnological interest. Syntrophic interactions among sulfate reducers and aceto/hydrogenotrophic and obligate hydrogenotrophic methanogens form a key component of these communities, yet, the impact of these different syntrophic routes on methane production and their stability against sulfate availability are not well understood. Here, we construct model synthetic communities using a sulfate reducer and two types of methanogens representing different methanogenesis routes. We find that tri-cultures with both routes increase methane production by almost twofold compared to co-cultures and are stable in the absence of sulfate. With increasing sulfate, system stability and productivity decreases and does so faster in communities with aceto/hydrogenotrophic methanogens despite the continued presence of acetate. We show that this is due to a shift in the metabolism of these methanogens towards co-utilization of hydrogen with acetate. These findings indicate the important role of hydrogen dynamics in the stability and productivity of syntrophic communities. The Royal Society 2019-05 2019-05-08 /pmc/articles/PMC6544901/ /pubmed/31064258 http://dx.doi.org/10.1098/rsif.2019.0129 Text en © 2019 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Life Sciences–Earth Science interface
Chen, Jing
Wade, Matthew J.
Dolfing, Jan
Soyer, Orkun S.
Increasing sulfate levels show a differential impact on synthetic communities comprising different methanogens and a sulfate reducer
title Increasing sulfate levels show a differential impact on synthetic communities comprising different methanogens and a sulfate reducer
title_full Increasing sulfate levels show a differential impact on synthetic communities comprising different methanogens and a sulfate reducer
title_fullStr Increasing sulfate levels show a differential impact on synthetic communities comprising different methanogens and a sulfate reducer
title_full_unstemmed Increasing sulfate levels show a differential impact on synthetic communities comprising different methanogens and a sulfate reducer
title_short Increasing sulfate levels show a differential impact on synthetic communities comprising different methanogens and a sulfate reducer
title_sort increasing sulfate levels show a differential impact on synthetic communities comprising different methanogens and a sulfate reducer
topic Life Sciences–Earth Science interface
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544901/
https://www.ncbi.nlm.nih.gov/pubmed/31064258
http://dx.doi.org/10.1098/rsif.2019.0129
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