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Maternal ancestry analyses of red tilapia strains based on D-loop sequences of seven tilapia populations

BACKGROUND: Many tilapia species or varieties have been widely introduced and have become an economically important food fish in China. Information on the genetic backgrounds of these populations is deficient and requires more research, especially for red tilapia strains. METHODS: In the present stu...

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Autores principales: Jiang, Bingjie, Fu, Jianjun, Dong, Zaijie, Fang, Min, Zhu, Wenbin, Wang, Lanmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6545098/
https://www.ncbi.nlm.nih.gov/pubmed/31179190
http://dx.doi.org/10.7717/peerj.7007
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author Jiang, Bingjie
Fu, Jianjun
Dong, Zaijie
Fang, Min
Zhu, Wenbin
Wang, Lanmei
author_facet Jiang, Bingjie
Fu, Jianjun
Dong, Zaijie
Fang, Min
Zhu, Wenbin
Wang, Lanmei
author_sort Jiang, Bingjie
collection PubMed
description BACKGROUND: Many tilapia species or varieties have been widely introduced and have become an economically important food fish in China. Information on the genetic backgrounds of these populations is deficient and requires more research, especially for red tilapia strains. METHODS: In the present study, displacement loop (D-loop) sequences were used to evaluate the genetic relationship and diversity of seven tilapia populations that are widely cultured in China; this was done specifically to speculate on the maternal ancestry of red tilapia strains. Three red tilapia varieties of Oreochromis ssp., Taiwan (TW), Israel (IL), and Malaysia (MY) strains and other populations, including O. aureus (AR), O. niloticus (NL), O. mossambicus (MS), and the GIFT strain of O. niloticus, were collected and analyzed in this study. RESULTS: A total of 146 polymorphic sites and 32 haplotypes of D-loop sequences were detected among 332 fish and four major haplotypes were shared among the populations. The TW and NL populations had a greater number of haplotypes (20 and 8, respectively). The haplotype diversity (Hd) and nucleotide diversity (π) of each population ranged from 0.234 to 0.826, and 0 to 0.060, respectively. The significant positive Tajima’s D value of neutral test were detected in the NL, IL, and MY populations (P < 0.05), which indicated these populations might have not experienced historical expansion. According to the pairwise F-statistics, highly significant genetic differentiations were detected among populations (P < 0.01), with the exception of the IL and MY populations (P > 0.05). The nearest K2P genetic distance (D = 0.014) was detected between the MS and TW populations, whereas, the farthest (D = 0.101) was found between the GIFT and AR populations. The results from the molecular variance analysis (AMOVA) showed that there was an extremely significant genetic variation observed among the populations (P < 0.01), which contained 63.57% of the total variation. In view of the genetic relationship of red tilapia strains with other populations, TW and IL were detected with more similar genetic structures related to MS, and MY was more genetically similar to GIFT (or NL), which could provide more genetic evidence for the red tilapia strains maternal ancestry.
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spelling pubmed-65450982019-06-09 Maternal ancestry analyses of red tilapia strains based on D-loop sequences of seven tilapia populations Jiang, Bingjie Fu, Jianjun Dong, Zaijie Fang, Min Zhu, Wenbin Wang, Lanmei PeerJ Aquaculture, Fisheries and Fish Science BACKGROUND: Many tilapia species or varieties have been widely introduced and have become an economically important food fish in China. Information on the genetic backgrounds of these populations is deficient and requires more research, especially for red tilapia strains. METHODS: In the present study, displacement loop (D-loop) sequences were used to evaluate the genetic relationship and diversity of seven tilapia populations that are widely cultured in China; this was done specifically to speculate on the maternal ancestry of red tilapia strains. Three red tilapia varieties of Oreochromis ssp., Taiwan (TW), Israel (IL), and Malaysia (MY) strains and other populations, including O. aureus (AR), O. niloticus (NL), O. mossambicus (MS), and the GIFT strain of O. niloticus, were collected and analyzed in this study. RESULTS: A total of 146 polymorphic sites and 32 haplotypes of D-loop sequences were detected among 332 fish and four major haplotypes were shared among the populations. The TW and NL populations had a greater number of haplotypes (20 and 8, respectively). The haplotype diversity (Hd) and nucleotide diversity (π) of each population ranged from 0.234 to 0.826, and 0 to 0.060, respectively. The significant positive Tajima’s D value of neutral test were detected in the NL, IL, and MY populations (P < 0.05), which indicated these populations might have not experienced historical expansion. According to the pairwise F-statistics, highly significant genetic differentiations were detected among populations (P < 0.01), with the exception of the IL and MY populations (P > 0.05). The nearest K2P genetic distance (D = 0.014) was detected between the MS and TW populations, whereas, the farthest (D = 0.101) was found between the GIFT and AR populations. The results from the molecular variance analysis (AMOVA) showed that there was an extremely significant genetic variation observed among the populations (P < 0.01), which contained 63.57% of the total variation. In view of the genetic relationship of red tilapia strains with other populations, TW and IL were detected with more similar genetic structures related to MS, and MY was more genetically similar to GIFT (or NL), which could provide more genetic evidence for the red tilapia strains maternal ancestry. PeerJ Inc. 2019-05-29 /pmc/articles/PMC6545098/ /pubmed/31179190 http://dx.doi.org/10.7717/peerj.7007 Text en ©2019 Jiang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Aquaculture, Fisheries and Fish Science
Jiang, Bingjie
Fu, Jianjun
Dong, Zaijie
Fang, Min
Zhu, Wenbin
Wang, Lanmei
Maternal ancestry analyses of red tilapia strains based on D-loop sequences of seven tilapia populations
title Maternal ancestry analyses of red tilapia strains based on D-loop sequences of seven tilapia populations
title_full Maternal ancestry analyses of red tilapia strains based on D-loop sequences of seven tilapia populations
title_fullStr Maternal ancestry analyses of red tilapia strains based on D-loop sequences of seven tilapia populations
title_full_unstemmed Maternal ancestry analyses of red tilapia strains based on D-loop sequences of seven tilapia populations
title_short Maternal ancestry analyses of red tilapia strains based on D-loop sequences of seven tilapia populations
title_sort maternal ancestry analyses of red tilapia strains based on d-loop sequences of seven tilapia populations
topic Aquaculture, Fisheries and Fish Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6545098/
https://www.ncbi.nlm.nih.gov/pubmed/31179190
http://dx.doi.org/10.7717/peerj.7007
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