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Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis

Cells going through mitosis undergo precisely timed changes in cell shape and organisation, which serve to ensure the fair partitioning of cellular components into the two daughter cells. These structural changes are driven by changes in actin filament and microtubule dynamics and organisation. Whil...

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Autores principales: Farina, Francesca, Ramkumar, Nitya, Brown, Louise, Samandar Eweis, Dureen, Anstatt, Jannis, Waring, Thomas, Bithell, Jessica, Scita, Giorgio, Thery, Manuel, Blanchoin, Laurent, Zech, Tobias, Baum, Buzz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6545563/
https://www.ncbi.nlm.nih.gov/pubmed/31015335
http://dx.doi.org/10.15252/embj.201899843
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author Farina, Francesca
Ramkumar, Nitya
Brown, Louise
Samandar Eweis, Dureen
Anstatt, Jannis
Waring, Thomas
Bithell, Jessica
Scita, Giorgio
Thery, Manuel
Blanchoin, Laurent
Zech, Tobias
Baum, Buzz
author_facet Farina, Francesca
Ramkumar, Nitya
Brown, Louise
Samandar Eweis, Dureen
Anstatt, Jannis
Waring, Thomas
Bithell, Jessica
Scita, Giorgio
Thery, Manuel
Blanchoin, Laurent
Zech, Tobias
Baum, Buzz
author_sort Farina, Francesca
collection PubMed
description Cells going through mitosis undergo precisely timed changes in cell shape and organisation, which serve to ensure the fair partitioning of cellular components into the two daughter cells. These structural changes are driven by changes in actin filament and microtubule dynamics and organisation. While most evidence suggests that the two cytoskeletal systems are remodelled in parallel during mitosis, recent work in interphase cells has implicated the centrosome in both microtubule and actin nucleation, suggesting the potential for regulatory crosstalk between the two systems. Here, by using both in vitro and in vivo assays to study centrosomal actin nucleation as cells pass through mitosis, we show that mitotic exit is accompanied by a burst in cytoplasmic actin filament formation that depends on WASH and the Arp2/3 complex. This leads to the accumulation of actin around centrosomes as cells enter anaphase and to a corresponding reduction in the density of centrosomal microtubules. Taken together, these data suggest that the mitotic regulation of centrosomal WASH and the Arp2/3 complex controls local actin nucleation, which may function to tune the levels of centrosomal microtubules during passage through mitosis.
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spelling pubmed-65455632019-06-05 Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis Farina, Francesca Ramkumar, Nitya Brown, Louise Samandar Eweis, Dureen Anstatt, Jannis Waring, Thomas Bithell, Jessica Scita, Giorgio Thery, Manuel Blanchoin, Laurent Zech, Tobias Baum, Buzz EMBO J Articles Cells going through mitosis undergo precisely timed changes in cell shape and organisation, which serve to ensure the fair partitioning of cellular components into the two daughter cells. These structural changes are driven by changes in actin filament and microtubule dynamics and organisation. While most evidence suggests that the two cytoskeletal systems are remodelled in parallel during mitosis, recent work in interphase cells has implicated the centrosome in both microtubule and actin nucleation, suggesting the potential for regulatory crosstalk between the two systems. Here, by using both in vitro and in vivo assays to study centrosomal actin nucleation as cells pass through mitosis, we show that mitotic exit is accompanied by a burst in cytoplasmic actin filament formation that depends on WASH and the Arp2/3 complex. This leads to the accumulation of actin around centrosomes as cells enter anaphase and to a corresponding reduction in the density of centrosomal microtubules. Taken together, these data suggest that the mitotic regulation of centrosomal WASH and the Arp2/3 complex controls local actin nucleation, which may function to tune the levels of centrosomal microtubules during passage through mitosis. John Wiley and Sons Inc. 2019-04-23 2019-06-03 /pmc/articles/PMC6545563/ /pubmed/31015335 http://dx.doi.org/10.15252/embj.201899843 Text en © 2019 The Authors. Published under the terms of the CC BY 4.0 license This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Farina, Francesca
Ramkumar, Nitya
Brown, Louise
Samandar Eweis, Dureen
Anstatt, Jannis
Waring, Thomas
Bithell, Jessica
Scita, Giorgio
Thery, Manuel
Blanchoin, Laurent
Zech, Tobias
Baum, Buzz
Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis
title Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis
title_full Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis
title_fullStr Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis
title_full_unstemmed Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis
title_short Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis
title_sort local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6545563/
https://www.ncbi.nlm.nih.gov/pubmed/31015335
http://dx.doi.org/10.15252/embj.201899843
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