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Intestinal luminal putrescine is produced by collective biosynthetic pathways of the commensal microbiome

The intestinal microbiome produces various metabolites that may harm or benefit the host. However, the production pathways of these metabolites have not been well characterised. The polyamines putrescine and spermidine required for physiological process are also produced by intestinal microbiome. Th...

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Autores principales: Nakamura, Atsuo, Ooga, Takushi, Matsumoto, Mitsuharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546329/
https://www.ncbi.nlm.nih.gov/pubmed/30183487
http://dx.doi.org/10.1080/19490976.2018.1494466
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author Nakamura, Atsuo
Ooga, Takushi
Matsumoto, Mitsuharu
author_facet Nakamura, Atsuo
Ooga, Takushi
Matsumoto, Mitsuharu
author_sort Nakamura, Atsuo
collection PubMed
description The intestinal microbiome produces various metabolites that may harm or benefit the host. However, the production pathways of these metabolites have not been well characterised. The polyamines putrescine and spermidine required for physiological process are also produced by intestinal microbiome. The production and release of these polyamines by microbiome are poorly understood, though we have confirmed that intestinal bacteria produced putrescine from arginine. In this study, we characterised polyamine synthesis by analysing the collective metabolic functions of the intestinal microbiome. In particular, we analysed polyamines and their intermediates in faecal cultures, as well as the colonic contents of rats injected with isotope-labelled arginine through a colon catheter, using mass spectrometry. Isotope-labelled putrescine was detected in faecal cultures and colonic contents of rats injected with isotope-labelled arginine. Putrescine is produced through multiple pathways, and its extracellular intermediates are exchanged between bacterial species. Additionally, we demonstrated that the collective metabolic pathway depends on a complex exchange of metabolites released into the colonic lumen. This study demonstrates the existence of putrescine biosynthetic pathways based on the collective metabolic functions of the intestinal microbial community. Our findings provide knowledge to manipulate the levels of intestinal microbial products, including polyamines, that may modulate host health.
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spelling pubmed-65463292019-06-14 Intestinal luminal putrescine is produced by collective biosynthetic pathways of the commensal microbiome Nakamura, Atsuo Ooga, Takushi Matsumoto, Mitsuharu Gut Microbes Research Paper/Report The intestinal microbiome produces various metabolites that may harm or benefit the host. However, the production pathways of these metabolites have not been well characterised. The polyamines putrescine and spermidine required for physiological process are also produced by intestinal microbiome. The production and release of these polyamines by microbiome are poorly understood, though we have confirmed that intestinal bacteria produced putrescine from arginine. In this study, we characterised polyamine synthesis by analysing the collective metabolic functions of the intestinal microbiome. In particular, we analysed polyamines and their intermediates in faecal cultures, as well as the colonic contents of rats injected with isotope-labelled arginine through a colon catheter, using mass spectrometry. Isotope-labelled putrescine was detected in faecal cultures and colonic contents of rats injected with isotope-labelled arginine. Putrescine is produced through multiple pathways, and its extracellular intermediates are exchanged between bacterial species. Additionally, we demonstrated that the collective metabolic pathway depends on a complex exchange of metabolites released into the colonic lumen. This study demonstrates the existence of putrescine biosynthetic pathways based on the collective metabolic functions of the intestinal microbial community. Our findings provide knowledge to manipulate the levels of intestinal microbial products, including polyamines, that may modulate host health. Taylor & Francis 2018-09-05 /pmc/articles/PMC6546329/ /pubmed/30183487 http://dx.doi.org/10.1080/19490976.2018.1494466 Text en © 2018 The Author(s). Published with license by Taylor & Francis Group, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Research Paper/Report
Nakamura, Atsuo
Ooga, Takushi
Matsumoto, Mitsuharu
Intestinal luminal putrescine is produced by collective biosynthetic pathways of the commensal microbiome
title Intestinal luminal putrescine is produced by collective biosynthetic pathways of the commensal microbiome
title_full Intestinal luminal putrescine is produced by collective biosynthetic pathways of the commensal microbiome
title_fullStr Intestinal luminal putrescine is produced by collective biosynthetic pathways of the commensal microbiome
title_full_unstemmed Intestinal luminal putrescine is produced by collective biosynthetic pathways of the commensal microbiome
title_short Intestinal luminal putrescine is produced by collective biosynthetic pathways of the commensal microbiome
title_sort intestinal luminal putrescine is produced by collective biosynthetic pathways of the commensal microbiome
topic Research Paper/Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546329/
https://www.ncbi.nlm.nih.gov/pubmed/30183487
http://dx.doi.org/10.1080/19490976.2018.1494466
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