Hair Cortisol Measurement by an Automated Method

We present the development of the first procedure for hair cortisol measurement through an automated method. Hair samples were obtained from 286 individuals. After cortisol extraction, samples were measured in a Siemens Immulite 2000 (Gwynedd, UK) automated chemoluminiscent immunoassay analyzer. Nor...

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Detalles Bibliográficos
Autores principales: Gonzalez, Diego, Jacobsen, Dario, Ibar, Carolina, Pavan, Carlos, Monti, José, Fernandez Machulsky, Nahuel, Balbi, Ayelen, Fritzler, Analy, Jamardo, Juan, Repetto, Esteban M., Berg, Gabriela, Fabre, Bibiana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546685/
https://www.ncbi.nlm.nih.gov/pubmed/31160639
http://dx.doi.org/10.1038/s41598-019-44693-3
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author Gonzalez, Diego
Jacobsen, Dario
Ibar, Carolina
Pavan, Carlos
Monti, José
Fernandez Machulsky, Nahuel
Balbi, Ayelen
Fritzler, Analy
Jamardo, Juan
Repetto, Esteban M.
Berg, Gabriela
Fabre, Bibiana
author_facet Gonzalez, Diego
Jacobsen, Dario
Ibar, Carolina
Pavan, Carlos
Monti, José
Fernandez Machulsky, Nahuel
Balbi, Ayelen
Fritzler, Analy
Jamardo, Juan
Repetto, Esteban M.
Berg, Gabriela
Fabre, Bibiana
author_sort Gonzalez, Diego
collection PubMed
description We present the development of the first procedure for hair cortisol measurement through an automated method. Hair samples were obtained from 286 individuals. After cortisol extraction, samples were measured in a Siemens Immulite 2000 (Gwynedd, UK) automated chemoluminiscent immunoassay analyzer. Normal reference values were obtained from hair cortisol levels measured in 213 healthy individuals with low levels of stress. Hair cortisol concentration median was 55 pg/mg hair (2.5–97.5 percentile (40–128)) in healthy individuals with low levels of stress and 250 pg/mg hair (range 182–520) in stressed individuals. No significant differences were observed in hair cortisol levels between subjects with and without dye (40 (40–107) and 40 (40–155) pg/mg hair, respectively; p = 0.128). The novel procedure presented here shows an adequate analytical performance.
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spelling pubmed-65466852019-06-10 Hair Cortisol Measurement by an Automated Method Gonzalez, Diego Jacobsen, Dario Ibar, Carolina Pavan, Carlos Monti, José Fernandez Machulsky, Nahuel Balbi, Ayelen Fritzler, Analy Jamardo, Juan Repetto, Esteban M. Berg, Gabriela Fabre, Bibiana Sci Rep Article We present the development of the first procedure for hair cortisol measurement through an automated method. Hair samples were obtained from 286 individuals. After cortisol extraction, samples were measured in a Siemens Immulite 2000 (Gwynedd, UK) automated chemoluminiscent immunoassay analyzer. Normal reference values were obtained from hair cortisol levels measured in 213 healthy individuals with low levels of stress. Hair cortisol concentration median was 55 pg/mg hair (2.5–97.5 percentile (40–128)) in healthy individuals with low levels of stress and 250 pg/mg hair (range 182–520) in stressed individuals. No significant differences were observed in hair cortisol levels between subjects with and without dye (40 (40–107) and 40 (40–155) pg/mg hair, respectively; p = 0.128). The novel procedure presented here shows an adequate analytical performance. Nature Publishing Group UK 2019-06-03 /pmc/articles/PMC6546685/ /pubmed/31160639 http://dx.doi.org/10.1038/s41598-019-44693-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Gonzalez, Diego
Jacobsen, Dario
Ibar, Carolina
Pavan, Carlos
Monti, José
Fernandez Machulsky, Nahuel
Balbi, Ayelen
Fritzler, Analy
Jamardo, Juan
Repetto, Esteban M.
Berg, Gabriela
Fabre, Bibiana
Hair Cortisol Measurement by an Automated Method
title Hair Cortisol Measurement by an Automated Method
title_full Hair Cortisol Measurement by an Automated Method
title_fullStr Hair Cortisol Measurement by an Automated Method
title_full_unstemmed Hair Cortisol Measurement by an Automated Method
title_short Hair Cortisol Measurement by an Automated Method
title_sort hair cortisol measurement by an automated method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546685/
https://www.ncbi.nlm.nih.gov/pubmed/31160639
http://dx.doi.org/10.1038/s41598-019-44693-3
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