Cargando…

Linc00423 as a tumor suppressor in retroperitoneal liposarcoma via activing MAPK signaling pathway through destabilizing of NFATC3

Unraveling the noncoding RNA expression networks governing cancer initiation and development is essential while remains largely uncompleted in retroperitoneal liposarcoma (RLS). Through RNA-seq technologies and computational biology, deregulated long noncoding RNAs (lncRNAs) are being identified and...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Yong, Tong, Hanxing, He, Junyi, Shao, Yebo, Guo, Xi, Zhuang, Rongyuan, Yang, Jue, Liu, Ju, Ding, Yuqin, Liu, Wenshuai, Lu, Weiqi, Zhou, Yuhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546787/
https://www.ncbi.nlm.nih.gov/pubmed/31160581
http://dx.doi.org/10.1038/s41419-019-1658-2
_version_ 1783423576944148480
author Zhang, Yong
Tong, Hanxing
He, Junyi
Shao, Yebo
Guo, Xi
Zhuang, Rongyuan
Yang, Jue
Liu, Ju
Ding, Yuqin
Liu, Wenshuai
Lu, Weiqi
Zhou, Yuhong
author_facet Zhang, Yong
Tong, Hanxing
He, Junyi
Shao, Yebo
Guo, Xi
Zhuang, Rongyuan
Yang, Jue
Liu, Ju
Ding, Yuqin
Liu, Wenshuai
Lu, Weiqi
Zhou, Yuhong
author_sort Zhang, Yong
collection PubMed
description Unraveling the noncoding RNA expression networks governing cancer initiation and development is essential while remains largely uncompleted in retroperitoneal liposarcoma (RLS). Through RNA-seq technologies and computational biology, deregulated long noncoding RNAs (lncRNAs) are being identified and reveal that lncRNAs are implicated in serial steps of RLS development. High-throughput sequencing with computational methods for assembling the transcriptome of five paired RLS patient’s tissues. We found that long intergenic noncoding RNA 423 (linc00423) was downregulated in RLS tissues. Gain-of-function assays revealed that overexpressed linc00423 obviously inhibited RLS cell growth in vitro and in vivo. Additionally, RNA sequence, RNA-pulldown and RIP assays evidenced that linc00423 involved in MAPK signaling pathway via destabilizing of nuclear factor of activated T-cells 3 (NFATC3). Summing up, our findings demonstrated that linc00423 acted as the tumor suppressor in RLS cells through regulating the protein level of NFATC3 at a post-transcriptional level and negatively regulated the MAPK signaling pathway at a transcriptional level. Linc00423 might serve as a candidate prognostic biomarker and a target for novel therapies of RLS patients.
format Online
Article
Text
id pubmed-6546787
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-65467872019-06-17 Linc00423 as a tumor suppressor in retroperitoneal liposarcoma via activing MAPK signaling pathway through destabilizing of NFATC3 Zhang, Yong Tong, Hanxing He, Junyi Shao, Yebo Guo, Xi Zhuang, Rongyuan Yang, Jue Liu, Ju Ding, Yuqin Liu, Wenshuai Lu, Weiqi Zhou, Yuhong Cell Death Dis Article Unraveling the noncoding RNA expression networks governing cancer initiation and development is essential while remains largely uncompleted in retroperitoneal liposarcoma (RLS). Through RNA-seq technologies and computational biology, deregulated long noncoding RNAs (lncRNAs) are being identified and reveal that lncRNAs are implicated in serial steps of RLS development. High-throughput sequencing with computational methods for assembling the transcriptome of five paired RLS patient’s tissues. We found that long intergenic noncoding RNA 423 (linc00423) was downregulated in RLS tissues. Gain-of-function assays revealed that overexpressed linc00423 obviously inhibited RLS cell growth in vitro and in vivo. Additionally, RNA sequence, RNA-pulldown and RIP assays evidenced that linc00423 involved in MAPK signaling pathway via destabilizing of nuclear factor of activated T-cells 3 (NFATC3). Summing up, our findings demonstrated that linc00423 acted as the tumor suppressor in RLS cells through regulating the protein level of NFATC3 at a post-transcriptional level and negatively regulated the MAPK signaling pathway at a transcriptional level. Linc00423 might serve as a candidate prognostic biomarker and a target for novel therapies of RLS patients. Nature Publishing Group UK 2019-06-03 /pmc/articles/PMC6546787/ /pubmed/31160581 http://dx.doi.org/10.1038/s41419-019-1658-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhang, Yong
Tong, Hanxing
He, Junyi
Shao, Yebo
Guo, Xi
Zhuang, Rongyuan
Yang, Jue
Liu, Ju
Ding, Yuqin
Liu, Wenshuai
Lu, Weiqi
Zhou, Yuhong
Linc00423 as a tumor suppressor in retroperitoneal liposarcoma via activing MAPK signaling pathway through destabilizing of NFATC3
title Linc00423 as a tumor suppressor in retroperitoneal liposarcoma via activing MAPK signaling pathway through destabilizing of NFATC3
title_full Linc00423 as a tumor suppressor in retroperitoneal liposarcoma via activing MAPK signaling pathway through destabilizing of NFATC3
title_fullStr Linc00423 as a tumor suppressor in retroperitoneal liposarcoma via activing MAPK signaling pathway through destabilizing of NFATC3
title_full_unstemmed Linc00423 as a tumor suppressor in retroperitoneal liposarcoma via activing MAPK signaling pathway through destabilizing of NFATC3
title_short Linc00423 as a tumor suppressor in retroperitoneal liposarcoma via activing MAPK signaling pathway through destabilizing of NFATC3
title_sort linc00423 as a tumor suppressor in retroperitoneal liposarcoma via activing mapk signaling pathway through destabilizing of nfatc3
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546787/
https://www.ncbi.nlm.nih.gov/pubmed/31160581
http://dx.doi.org/10.1038/s41419-019-1658-2
work_keys_str_mv AT zhangyong linc00423asatumorsuppressorinretroperitonealliposarcomaviaactivingmapksignalingpathwaythroughdestabilizingofnfatc3
AT tonghanxing linc00423asatumorsuppressorinretroperitonealliposarcomaviaactivingmapksignalingpathwaythroughdestabilizingofnfatc3
AT hejunyi linc00423asatumorsuppressorinretroperitonealliposarcomaviaactivingmapksignalingpathwaythroughdestabilizingofnfatc3
AT shaoyebo linc00423asatumorsuppressorinretroperitonealliposarcomaviaactivingmapksignalingpathwaythroughdestabilizingofnfatc3
AT guoxi linc00423asatumorsuppressorinretroperitonealliposarcomaviaactivingmapksignalingpathwaythroughdestabilizingofnfatc3
AT zhuangrongyuan linc00423asatumorsuppressorinretroperitonealliposarcomaviaactivingmapksignalingpathwaythroughdestabilizingofnfatc3
AT yangjue linc00423asatumorsuppressorinretroperitonealliposarcomaviaactivingmapksignalingpathwaythroughdestabilizingofnfatc3
AT liuju linc00423asatumorsuppressorinretroperitonealliposarcomaviaactivingmapksignalingpathwaythroughdestabilizingofnfatc3
AT dingyuqin linc00423asatumorsuppressorinretroperitonealliposarcomaviaactivingmapksignalingpathwaythroughdestabilizingofnfatc3
AT liuwenshuai linc00423asatumorsuppressorinretroperitonealliposarcomaviaactivingmapksignalingpathwaythroughdestabilizingofnfatc3
AT luweiqi linc00423asatumorsuppressorinretroperitonealliposarcomaviaactivingmapksignalingpathwaythroughdestabilizingofnfatc3
AT zhouyuhong linc00423asatumorsuppressorinretroperitonealliposarcomaviaactivingmapksignalingpathwaythroughdestabilizingofnfatc3