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Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis

Tuberculosis (TB) has been the leading lethal infectious disease worldwide since 2014, and about one third of the world’s population has a latent TB infection (LTBI). This is largely attributed to the difficulties in diagnosis and treatment of TB and LTBI patients. Exosomes offer a new perspective o...

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Autores principales: Lyu, Lingna, Zhang, Xiuli, Li, Cuidan, Yang, Tingting, Wang, Jinghui, Pan, Liping, Jia, Hongyan, Li, Zihui, Sun, Qi, Yue, Liya, Chen, Fei, Zhang, Zongde
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546874/
https://www.ncbi.nlm.nih.gov/pubmed/31191492
http://dx.doi.org/10.3389/fmicb.2019.01174
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author Lyu, Lingna
Zhang, Xiuli
Li, Cuidan
Yang, Tingting
Wang, Jinghui
Pan, Liping
Jia, Hongyan
Li, Zihui
Sun, Qi
Yue, Liya
Chen, Fei
Zhang, Zongde
author_facet Lyu, Lingna
Zhang, Xiuli
Li, Cuidan
Yang, Tingting
Wang, Jinghui
Pan, Liping
Jia, Hongyan
Li, Zihui
Sun, Qi
Yue, Liya
Chen, Fei
Zhang, Zongde
author_sort Lyu, Lingna
collection PubMed
description Tuberculosis (TB) has been the leading lethal infectious disease worldwide since 2014, and about one third of the world’s population has a latent TB infection (LTBI). This is largely attributed to the difficulties in diagnosis and treatment of TB and LTBI patients. Exosomes offer a new perspective on investigation of the process of TB infection. In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC). Our results revealed distinct miRNA profile of the exosomes in the three groups. We screened 250 differentially expressed miRNAs including 130 specifically expressed miRNAs. Some miRNAs were further validated to be specifically expressed in LTBI (hsa-let-7e-5p, hsa-let-7d-5p, hsa-miR-450a-5p, and hsa-miR-140-5p) and TB samples (hsa-miR-1246, hsa-miR-2110, hsa-miR-370-3P, hsa-miR-28-3p, and hsa-miR-193b-5p). Additionally, we demonstrated four expression panels in LTBI and TB groups, and six expression patterns among the three groups. These specifically expressed miRNAs and differentially expressed miRNAs in different panels and patterns provide potential biomarkers for detection/diagnosis of latent and active TB using exosomal miRNAs. Additionally, we also discovered plenty of small RNAs derived from genomic repetitive sequences, which might play roles in host immune responses along with Mtb infection progresses. Overall, our findings provide important reference and an improved understanding about miRNAs and repetitive region-derived small RNAs in exosomes during the Mtb infectious process, and facilitate the development of potential molecular targets for detection/diagnosis of latent and active tuberculosis.
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spelling pubmed-65468742019-06-12 Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Lyu, Lingna Zhang, Xiuli Li, Cuidan Yang, Tingting Wang, Jinghui Pan, Liping Jia, Hongyan Li, Zihui Sun, Qi Yue, Liya Chen, Fei Zhang, Zongde Front Microbiol Microbiology Tuberculosis (TB) has been the leading lethal infectious disease worldwide since 2014, and about one third of the world’s population has a latent TB infection (LTBI). This is largely attributed to the difficulties in diagnosis and treatment of TB and LTBI patients. Exosomes offer a new perspective on investigation of the process of TB infection. In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC). Our results revealed distinct miRNA profile of the exosomes in the three groups. We screened 250 differentially expressed miRNAs including 130 specifically expressed miRNAs. Some miRNAs were further validated to be specifically expressed in LTBI (hsa-let-7e-5p, hsa-let-7d-5p, hsa-miR-450a-5p, and hsa-miR-140-5p) and TB samples (hsa-miR-1246, hsa-miR-2110, hsa-miR-370-3P, hsa-miR-28-3p, and hsa-miR-193b-5p). Additionally, we demonstrated four expression panels in LTBI and TB groups, and six expression patterns among the three groups. These specifically expressed miRNAs and differentially expressed miRNAs in different panels and patterns provide potential biomarkers for detection/diagnosis of latent and active TB using exosomal miRNAs. Additionally, we also discovered plenty of small RNAs derived from genomic repetitive sequences, which might play roles in host immune responses along with Mtb infection progresses. Overall, our findings provide important reference and an improved understanding about miRNAs and repetitive region-derived small RNAs in exosomes during the Mtb infectious process, and facilitate the development of potential molecular targets for detection/diagnosis of latent and active tuberculosis. Frontiers Media S.A. 2019-05-28 /pmc/articles/PMC6546874/ /pubmed/31191492 http://dx.doi.org/10.3389/fmicb.2019.01174 Text en Copyright © 2019 Lyu, Zhang, Li, Yang, Wang, Pan, Jia, Li, Sun, Yue, Chen and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lyu, Lingna
Zhang, Xiuli
Li, Cuidan
Yang, Tingting
Wang, Jinghui
Pan, Liping
Jia, Hongyan
Li, Zihui
Sun, Qi
Yue, Liya
Chen, Fei
Zhang, Zongde
Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis
title Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis
title_full Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis
title_fullStr Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis
title_full_unstemmed Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis
title_short Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis
title_sort small rna profiles of serum exosomes derived from individuals with latent and active tuberculosis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546874/
https://www.ncbi.nlm.nih.gov/pubmed/31191492
http://dx.doi.org/10.3389/fmicb.2019.01174
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