Cargando…
Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis
Tuberculosis (TB) has been the leading lethal infectious disease worldwide since 2014, and about one third of the world’s population has a latent TB infection (LTBI). This is largely attributed to the difficulties in diagnosis and treatment of TB and LTBI patients. Exosomes offer a new perspective o...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546874/ https://www.ncbi.nlm.nih.gov/pubmed/31191492 http://dx.doi.org/10.3389/fmicb.2019.01174 |
_version_ | 1783423593228533760 |
---|---|
author | Lyu, Lingna Zhang, Xiuli Li, Cuidan Yang, Tingting Wang, Jinghui Pan, Liping Jia, Hongyan Li, Zihui Sun, Qi Yue, Liya Chen, Fei Zhang, Zongde |
author_facet | Lyu, Lingna Zhang, Xiuli Li, Cuidan Yang, Tingting Wang, Jinghui Pan, Liping Jia, Hongyan Li, Zihui Sun, Qi Yue, Liya Chen, Fei Zhang, Zongde |
author_sort | Lyu, Lingna |
collection | PubMed |
description | Tuberculosis (TB) has been the leading lethal infectious disease worldwide since 2014, and about one third of the world’s population has a latent TB infection (LTBI). This is largely attributed to the difficulties in diagnosis and treatment of TB and LTBI patients. Exosomes offer a new perspective on investigation of the process of TB infection. In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC). Our results revealed distinct miRNA profile of the exosomes in the three groups. We screened 250 differentially expressed miRNAs including 130 specifically expressed miRNAs. Some miRNAs were further validated to be specifically expressed in LTBI (hsa-let-7e-5p, hsa-let-7d-5p, hsa-miR-450a-5p, and hsa-miR-140-5p) and TB samples (hsa-miR-1246, hsa-miR-2110, hsa-miR-370-3P, hsa-miR-28-3p, and hsa-miR-193b-5p). Additionally, we demonstrated four expression panels in LTBI and TB groups, and six expression patterns among the three groups. These specifically expressed miRNAs and differentially expressed miRNAs in different panels and patterns provide potential biomarkers for detection/diagnosis of latent and active TB using exosomal miRNAs. Additionally, we also discovered plenty of small RNAs derived from genomic repetitive sequences, which might play roles in host immune responses along with Mtb infection progresses. Overall, our findings provide important reference and an improved understanding about miRNAs and repetitive region-derived small RNAs in exosomes during the Mtb infectious process, and facilitate the development of potential molecular targets for detection/diagnosis of latent and active tuberculosis. |
format | Online Article Text |
id | pubmed-6546874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65468742019-06-12 Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Lyu, Lingna Zhang, Xiuli Li, Cuidan Yang, Tingting Wang, Jinghui Pan, Liping Jia, Hongyan Li, Zihui Sun, Qi Yue, Liya Chen, Fei Zhang, Zongde Front Microbiol Microbiology Tuberculosis (TB) has been the leading lethal infectious disease worldwide since 2014, and about one third of the world’s population has a latent TB infection (LTBI). This is largely attributed to the difficulties in diagnosis and treatment of TB and LTBI patients. Exosomes offer a new perspective on investigation of the process of TB infection. In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC). Our results revealed distinct miRNA profile of the exosomes in the three groups. We screened 250 differentially expressed miRNAs including 130 specifically expressed miRNAs. Some miRNAs were further validated to be specifically expressed in LTBI (hsa-let-7e-5p, hsa-let-7d-5p, hsa-miR-450a-5p, and hsa-miR-140-5p) and TB samples (hsa-miR-1246, hsa-miR-2110, hsa-miR-370-3P, hsa-miR-28-3p, and hsa-miR-193b-5p). Additionally, we demonstrated four expression panels in LTBI and TB groups, and six expression patterns among the three groups. These specifically expressed miRNAs and differentially expressed miRNAs in different panels and patterns provide potential biomarkers for detection/diagnosis of latent and active TB using exosomal miRNAs. Additionally, we also discovered plenty of small RNAs derived from genomic repetitive sequences, which might play roles in host immune responses along with Mtb infection progresses. Overall, our findings provide important reference and an improved understanding about miRNAs and repetitive region-derived small RNAs in exosomes during the Mtb infectious process, and facilitate the development of potential molecular targets for detection/diagnosis of latent and active tuberculosis. Frontiers Media S.A. 2019-05-28 /pmc/articles/PMC6546874/ /pubmed/31191492 http://dx.doi.org/10.3389/fmicb.2019.01174 Text en Copyright © 2019 Lyu, Zhang, Li, Yang, Wang, Pan, Jia, Li, Sun, Yue, Chen and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Lyu, Lingna Zhang, Xiuli Li, Cuidan Yang, Tingting Wang, Jinghui Pan, Liping Jia, Hongyan Li, Zihui Sun, Qi Yue, Liya Chen, Fei Zhang, Zongde Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis |
title | Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis |
title_full | Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis |
title_fullStr | Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis |
title_full_unstemmed | Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis |
title_short | Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis |
title_sort | small rna profiles of serum exosomes derived from individuals with latent and active tuberculosis |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546874/ https://www.ncbi.nlm.nih.gov/pubmed/31191492 http://dx.doi.org/10.3389/fmicb.2019.01174 |
work_keys_str_mv | AT lyulingna smallrnaprofilesofserumexosomesderivedfromindividualswithlatentandactivetuberculosis AT zhangxiuli smallrnaprofilesofserumexosomesderivedfromindividualswithlatentandactivetuberculosis AT licuidan smallrnaprofilesofserumexosomesderivedfromindividualswithlatentandactivetuberculosis AT yangtingting smallrnaprofilesofserumexosomesderivedfromindividualswithlatentandactivetuberculosis AT wangjinghui smallrnaprofilesofserumexosomesderivedfromindividualswithlatentandactivetuberculosis AT panliping smallrnaprofilesofserumexosomesderivedfromindividualswithlatentandactivetuberculosis AT jiahongyan smallrnaprofilesofserumexosomesderivedfromindividualswithlatentandactivetuberculosis AT lizihui smallrnaprofilesofserumexosomesderivedfromindividualswithlatentandactivetuberculosis AT sunqi smallrnaprofilesofserumexosomesderivedfromindividualswithlatentandactivetuberculosis AT yueliya smallrnaprofilesofserumexosomesderivedfromindividualswithlatentandactivetuberculosis AT chenfei smallrnaprofilesofserumexosomesderivedfromindividualswithlatentandactivetuberculosis AT zhangzongde smallrnaprofilesofserumexosomesderivedfromindividualswithlatentandactivetuberculosis |