Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes

Replication-Factor-C (RFC) and RFC-like complexes (RLCs) mediate chromatin engagement of the proliferating cell nuclear antigen (PCNA). It remains controversial how RFC and RLCs cooperate to regulate PCNA loading and unloading. Here, we show the distinct PCNA loading or unloading activity of each cl...

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Autores principales: Kang, Mi-Sun, Ryu, Eunjin, Lee, Seung-Won, Park, Jieun, Ha, Na Young, Ra, Jae Sun, Kim, Yeong Jae, Kim, Jinwoo, Abdel-Rahman, Mohamed, Park, Su Hyung, Lee, Kyoo-young, Kim, Hajin, Kang, Sukhyun, Myung, Kyungjae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546911/
https://www.ncbi.nlm.nih.gov/pubmed/31160570
http://dx.doi.org/10.1038/s41467-019-10376-w
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author Kang, Mi-Sun
Ryu, Eunjin
Lee, Seung-Won
Park, Jieun
Ha, Na Young
Ra, Jae Sun
Kim, Yeong Jae
Kim, Jinwoo
Abdel-Rahman, Mohamed
Park, Su Hyung
Lee, Kyoo-young
Kim, Hajin
Kang, Sukhyun
Myung, Kyungjae
author_facet Kang, Mi-Sun
Ryu, Eunjin
Lee, Seung-Won
Park, Jieun
Ha, Na Young
Ra, Jae Sun
Kim, Yeong Jae
Kim, Jinwoo
Abdel-Rahman, Mohamed
Park, Su Hyung
Lee, Kyoo-young
Kim, Hajin
Kang, Sukhyun
Myung, Kyungjae
author_sort Kang, Mi-Sun
collection PubMed
description Replication-Factor-C (RFC) and RFC-like complexes (RLCs) mediate chromatin engagement of the proliferating cell nuclear antigen (PCNA). It remains controversial how RFC and RLCs cooperate to regulate PCNA loading and unloading. Here, we show the distinct PCNA loading or unloading activity of each clamp loader. ATAD5-RLC possesses the potent PCNA unloading activity. ATPase motif and collar domain of ATAD5 are crucial for the unloading activity. DNA structures did not affect PCNA unloading activity of ATAD5-RLC. ATAD5-RLC could unload ubiquitinated PCNA. Through single molecule measurements, we reveal that ATAD5-RLC unloaded PCNA through one intermediate state before ATP hydrolysis. RFC loaded PCNA through two intermediate states on DNA, separated by ATP hydrolysis. Replication proteins such as Fen1 could inhibit the PCNA unloading activity of Elg1-RLC, a yeast homolog of ATAD5-RLC in vitro. Our findings provide molecular insights into how PCNA is released from chromatin to finalize DNA replication/repair.
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spelling pubmed-65469112019-06-18 Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes Kang, Mi-Sun Ryu, Eunjin Lee, Seung-Won Park, Jieun Ha, Na Young Ra, Jae Sun Kim, Yeong Jae Kim, Jinwoo Abdel-Rahman, Mohamed Park, Su Hyung Lee, Kyoo-young Kim, Hajin Kang, Sukhyun Myung, Kyungjae Nat Commun Article Replication-Factor-C (RFC) and RFC-like complexes (RLCs) mediate chromatin engagement of the proliferating cell nuclear antigen (PCNA). It remains controversial how RFC and RLCs cooperate to regulate PCNA loading and unloading. Here, we show the distinct PCNA loading or unloading activity of each clamp loader. ATAD5-RLC possesses the potent PCNA unloading activity. ATPase motif and collar domain of ATAD5 are crucial for the unloading activity. DNA structures did not affect PCNA unloading activity of ATAD5-RLC. ATAD5-RLC could unload ubiquitinated PCNA. Through single molecule measurements, we reveal that ATAD5-RLC unloaded PCNA through one intermediate state before ATP hydrolysis. RFC loaded PCNA through two intermediate states on DNA, separated by ATP hydrolysis. Replication proteins such as Fen1 could inhibit the PCNA unloading activity of Elg1-RLC, a yeast homolog of ATAD5-RLC in vitro. Our findings provide molecular insights into how PCNA is released from chromatin to finalize DNA replication/repair. Nature Publishing Group UK 2019-06-03 /pmc/articles/PMC6546911/ /pubmed/31160570 http://dx.doi.org/10.1038/s41467-019-10376-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kang, Mi-Sun
Ryu, Eunjin
Lee, Seung-Won
Park, Jieun
Ha, Na Young
Ra, Jae Sun
Kim, Yeong Jae
Kim, Jinwoo
Abdel-Rahman, Mohamed
Park, Su Hyung
Lee, Kyoo-young
Kim, Hajin
Kang, Sukhyun
Myung, Kyungjae
Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes
title Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes
title_full Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes
title_fullStr Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes
title_full_unstemmed Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes
title_short Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes
title_sort regulation of pcna cycling on replicating dna by rfc and rfc-like complexes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546911/
https://www.ncbi.nlm.nih.gov/pubmed/31160570
http://dx.doi.org/10.1038/s41467-019-10376-w
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