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Global serum proteomic changes in water buffaloes infected with Fasciola gigantica

BACKGROUND: The liver fluke Fasciola gigantica modulates several signaling pathways in infected buffaloes to facilitate its survival and establishment of persistent infection. In response to the parasite invasion, buffaloes activate innate and adaptive immune responses to counter the parasite infect...

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Autores principales: Zhang, Fu-Kai, Hu, Rui-Si, Elsheikha, Hany M., Sheng, Zhao-An, Zhang, Wei-Yu, Zheng, Wen-Bin, Zhu, Xing-Quan, He, Jun-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6547537/
https://www.ncbi.nlm.nih.gov/pubmed/31159882
http://dx.doi.org/10.1186/s13071-019-3533-5
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author Zhang, Fu-Kai
Hu, Rui-Si
Elsheikha, Hany M.
Sheng, Zhao-An
Zhang, Wei-Yu
Zheng, Wen-Bin
Zhu, Xing-Quan
He, Jun-Jun
author_facet Zhang, Fu-Kai
Hu, Rui-Si
Elsheikha, Hany M.
Sheng, Zhao-An
Zhang, Wei-Yu
Zheng, Wen-Bin
Zhu, Xing-Quan
He, Jun-Jun
author_sort Zhang, Fu-Kai
collection PubMed
description BACKGROUND: The liver fluke Fasciola gigantica modulates several signaling pathways in infected buffaloes to facilitate its survival and establishment of persistent infection. In response to the parasite invasion, buffaloes activate innate and adaptive immune responses to counter the parasite infection. To detect new proteins that might be involved in the interaction between F. gigantica and the buffaloes, and that also might serve as biomarkers for fasciolosis, we used proteomic techniques to study the serum proteome of buffaloes during F. gigantica infection. Here, we used an isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic approach to identify serum proteins that are differentially expressed in infected buffaloes compared to uninfected control buffaloes. Additionally, we applied a parallel reaction monitoring (PRM) assay to validate specific proteins identified by the iTRAQ method. RESULTS: A total of 313, 459 and 399 proteins were identified at 3, 42 and 70 days post-infection, respectively; of these 92, 93 and 138 were differentially abundant proteins. Some of the identified differentially abundant proteins, including complement factor H related 5, complement component C6, complement component C7, amine oxidase, plasma serine protease inhibitor and lysozyme, are known to be involved in complement system activation, blood coagulation, platelet activation, lymphocyte’s adhesion and lysozyme hydrolysis. Analysis of data for all three time points after infection identified six significantly upregulated proteins in infected serum that separated infected and uninfected buffaloes into distinct clusters. Further PRM analysis confirmed the expression of five proteins, namely MHC class I antigen, Beta-2-microglobulin, NID2 protein, Fetuin-B and Fibrinogen gamma-B chain. CONCLUSIONS: These findings provide novel insights into the serum proteomics signature of buffaloes during F. gigantica infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-019-3533-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-65475372019-06-06 Global serum proteomic changes in water buffaloes infected with Fasciola gigantica Zhang, Fu-Kai Hu, Rui-Si Elsheikha, Hany M. Sheng, Zhao-An Zhang, Wei-Yu Zheng, Wen-Bin Zhu, Xing-Quan He, Jun-Jun Parasit Vectors Research BACKGROUND: The liver fluke Fasciola gigantica modulates several signaling pathways in infected buffaloes to facilitate its survival and establishment of persistent infection. In response to the parasite invasion, buffaloes activate innate and adaptive immune responses to counter the parasite infection. To detect new proteins that might be involved in the interaction between F. gigantica and the buffaloes, and that also might serve as biomarkers for fasciolosis, we used proteomic techniques to study the serum proteome of buffaloes during F. gigantica infection. Here, we used an isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic approach to identify serum proteins that are differentially expressed in infected buffaloes compared to uninfected control buffaloes. Additionally, we applied a parallel reaction monitoring (PRM) assay to validate specific proteins identified by the iTRAQ method. RESULTS: A total of 313, 459 and 399 proteins were identified at 3, 42 and 70 days post-infection, respectively; of these 92, 93 and 138 were differentially abundant proteins. Some of the identified differentially abundant proteins, including complement factor H related 5, complement component C6, complement component C7, amine oxidase, plasma serine protease inhibitor and lysozyme, are known to be involved in complement system activation, blood coagulation, platelet activation, lymphocyte’s adhesion and lysozyme hydrolysis. Analysis of data for all three time points after infection identified six significantly upregulated proteins in infected serum that separated infected and uninfected buffaloes into distinct clusters. Further PRM analysis confirmed the expression of five proteins, namely MHC class I antigen, Beta-2-microglobulin, NID2 protein, Fetuin-B and Fibrinogen gamma-B chain. CONCLUSIONS: These findings provide novel insights into the serum proteomics signature of buffaloes during F. gigantica infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-019-3533-5) contains supplementary material, which is available to authorized users. BioMed Central 2019-06-03 /pmc/articles/PMC6547537/ /pubmed/31159882 http://dx.doi.org/10.1186/s13071-019-3533-5 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Zhang, Fu-Kai
Hu, Rui-Si
Elsheikha, Hany M.
Sheng, Zhao-An
Zhang, Wei-Yu
Zheng, Wen-Bin
Zhu, Xing-Quan
He, Jun-Jun
Global serum proteomic changes in water buffaloes infected with Fasciola gigantica
title Global serum proteomic changes in water buffaloes infected with Fasciola gigantica
title_full Global serum proteomic changes in water buffaloes infected with Fasciola gigantica
title_fullStr Global serum proteomic changes in water buffaloes infected with Fasciola gigantica
title_full_unstemmed Global serum proteomic changes in water buffaloes infected with Fasciola gigantica
title_short Global serum proteomic changes in water buffaloes infected with Fasciola gigantica
title_sort global serum proteomic changes in water buffaloes infected with fasciola gigantica
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6547537/
https://www.ncbi.nlm.nih.gov/pubmed/31159882
http://dx.doi.org/10.1186/s13071-019-3533-5
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