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A Growth-Based Framework for Leaf Shape Development and Diversity

How do genes modify cellular growth to create morphological diversity? We study this problem in two related plants with differently shaped leaves: Arabidopsis thaliana (simple leaf shape) and Cardamine hirsuta (complex shape with leaflets). We use live imaging, modeling, and genetics to deconstruct...

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Autores principales: Kierzkowski, Daniel, Runions, Adam, Vuolo, Francesco, Strauss, Sören, Lymbouridou, Rena, Routier-Kierzkowska, Anne-Lise, Wilson-Sánchez, David, Jenke, Hannah, Galinha, Carla, Mosca, Gabriella, Zhang, Zhongjuan, Canales, Claudia, Dello Ioio, Raffaele, Huijser, Peter, Smith, Richard S., Tsiantis, Miltos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548024/
https://www.ncbi.nlm.nih.gov/pubmed/31130379
http://dx.doi.org/10.1016/j.cell.2019.05.011
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author Kierzkowski, Daniel
Runions, Adam
Vuolo, Francesco
Strauss, Sören
Lymbouridou, Rena
Routier-Kierzkowska, Anne-Lise
Wilson-Sánchez, David
Jenke, Hannah
Galinha, Carla
Mosca, Gabriella
Zhang, Zhongjuan
Canales, Claudia
Dello Ioio, Raffaele
Huijser, Peter
Smith, Richard S.
Tsiantis, Miltos
author_facet Kierzkowski, Daniel
Runions, Adam
Vuolo, Francesco
Strauss, Sören
Lymbouridou, Rena
Routier-Kierzkowska, Anne-Lise
Wilson-Sánchez, David
Jenke, Hannah
Galinha, Carla
Mosca, Gabriella
Zhang, Zhongjuan
Canales, Claudia
Dello Ioio, Raffaele
Huijser, Peter
Smith, Richard S.
Tsiantis, Miltos
author_sort Kierzkowski, Daniel
collection PubMed
description How do genes modify cellular growth to create morphological diversity? We study this problem in two related plants with differently shaped leaves: Arabidopsis thaliana (simple leaf shape) and Cardamine hirsuta (complex shape with leaflets). We use live imaging, modeling, and genetics to deconstruct these organ-level differences into their cell-level constituents: growth amount, direction, and differentiation. We show that leaf shape depends on the interplay of two growth modes: a conserved organ-wide growth mode that reflects differentiation; and a local, directional mode that involves the patterning of growth foci along the leaf edge. Shape diversity results from the distinct effects of two homeobox genes on these growth modes: SHOOTMERISTEMLESS broadens organ-wide growth relative to edge-patterning, enabling leaflet emergence, while REDUCED COMPLEXITY inhibits growth locally around emerging leaflets, accentuating shape differences created by patterning. We demonstrate the predictivity of our findings by reconstructing key features of C. hirsuta leaf morphology in A. thaliana. VIDEO ABSTRACT:
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spelling pubmed-65480242019-06-06 A Growth-Based Framework for Leaf Shape Development and Diversity Kierzkowski, Daniel Runions, Adam Vuolo, Francesco Strauss, Sören Lymbouridou, Rena Routier-Kierzkowska, Anne-Lise Wilson-Sánchez, David Jenke, Hannah Galinha, Carla Mosca, Gabriella Zhang, Zhongjuan Canales, Claudia Dello Ioio, Raffaele Huijser, Peter Smith, Richard S. Tsiantis, Miltos Cell Article How do genes modify cellular growth to create morphological diversity? We study this problem in two related plants with differently shaped leaves: Arabidopsis thaliana (simple leaf shape) and Cardamine hirsuta (complex shape with leaflets). We use live imaging, modeling, and genetics to deconstruct these organ-level differences into their cell-level constituents: growth amount, direction, and differentiation. We show that leaf shape depends on the interplay of two growth modes: a conserved organ-wide growth mode that reflects differentiation; and a local, directional mode that involves the patterning of growth foci along the leaf edge. Shape diversity results from the distinct effects of two homeobox genes on these growth modes: SHOOTMERISTEMLESS broadens organ-wide growth relative to edge-patterning, enabling leaflet emergence, while REDUCED COMPLEXITY inhibits growth locally around emerging leaflets, accentuating shape differences created by patterning. We demonstrate the predictivity of our findings by reconstructing key features of C. hirsuta leaf morphology in A. thaliana. VIDEO ABSTRACT: Cell Press 2019-05-30 /pmc/articles/PMC6548024/ /pubmed/31130379 http://dx.doi.org/10.1016/j.cell.2019.05.011 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kierzkowski, Daniel
Runions, Adam
Vuolo, Francesco
Strauss, Sören
Lymbouridou, Rena
Routier-Kierzkowska, Anne-Lise
Wilson-Sánchez, David
Jenke, Hannah
Galinha, Carla
Mosca, Gabriella
Zhang, Zhongjuan
Canales, Claudia
Dello Ioio, Raffaele
Huijser, Peter
Smith, Richard S.
Tsiantis, Miltos
A Growth-Based Framework for Leaf Shape Development and Diversity
title A Growth-Based Framework for Leaf Shape Development and Diversity
title_full A Growth-Based Framework for Leaf Shape Development and Diversity
title_fullStr A Growth-Based Framework for Leaf Shape Development and Diversity
title_full_unstemmed A Growth-Based Framework for Leaf Shape Development and Diversity
title_short A Growth-Based Framework for Leaf Shape Development and Diversity
title_sort growth-based framework for leaf shape development and diversity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548024/
https://www.ncbi.nlm.nih.gov/pubmed/31130379
http://dx.doi.org/10.1016/j.cell.2019.05.011
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