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Proteome of the central apparatus of a ciliary axoneme

Nearly all motile cilia have a “9+2” axoneme containing a central apparatus (CA), consisting of two central microtubules with projections, that is essential for motility. To date, only 22 proteins are known to be CA components. To identify new candidate CA proteins, we used mass spectrometry to comp...

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Detalles Bibliográficos
Autores principales: Zhao, Lei, Hou, Yuqing, Picariello, Tyler, Craige, Branch, Witman, George B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548120/
https://www.ncbi.nlm.nih.gov/pubmed/31092556
http://dx.doi.org/10.1083/jcb.201902017
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author Zhao, Lei
Hou, Yuqing
Picariello, Tyler
Craige, Branch
Witman, George B.
author_facet Zhao, Lei
Hou, Yuqing
Picariello, Tyler
Craige, Branch
Witman, George B.
author_sort Zhao, Lei
collection PubMed
description Nearly all motile cilia have a “9+2” axoneme containing a central apparatus (CA), consisting of two central microtubules with projections, that is essential for motility. To date, only 22 proteins are known to be CA components. To identify new candidate CA proteins, we used mass spectrometry to compare axonemes of wild-type Chlamydomonas and a CA-less mutant. We identified 44 novel candidate CA proteins, of which 13 are conserved in humans. Five of the latter were studied more closely, and all five localized to the CA; therefore, most of the other candidates are likely to also be CA components. Our results reveal that the CA is far more compositionally complex than previously recognized and provide a greatly expanded knowledge base for studies to understand the architecture of the CA and how it functions. The discovery of the new conserved CA proteins will facilitate genetic screening to identify patients with a form of primary ciliary dyskinesia that has been difficult to diagnose.
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spelling pubmed-65481202019-12-28 Proteome of the central apparatus of a ciliary axoneme Zhao, Lei Hou, Yuqing Picariello, Tyler Craige, Branch Witman, George B. J Cell Biol Research Articles Nearly all motile cilia have a “9+2” axoneme containing a central apparatus (CA), consisting of two central microtubules with projections, that is essential for motility. To date, only 22 proteins are known to be CA components. To identify new candidate CA proteins, we used mass spectrometry to compare axonemes of wild-type Chlamydomonas and a CA-less mutant. We identified 44 novel candidate CA proteins, of which 13 are conserved in humans. Five of the latter were studied more closely, and all five localized to the CA; therefore, most of the other candidates are likely to also be CA components. Our results reveal that the CA is far more compositionally complex than previously recognized and provide a greatly expanded knowledge base for studies to understand the architecture of the CA and how it functions. The discovery of the new conserved CA proteins will facilitate genetic screening to identify patients with a form of primary ciliary dyskinesia that has been difficult to diagnose. Rockefeller University Press 2019-06-28 2019-05-15 /pmc/articles/PMC6548120/ /pubmed/31092556 http://dx.doi.org/10.1083/jcb.201902017 Text en © 2019 Zhao et al. https://creativecommons.org/licenses/by-nc-sa/4.0/http://www.rupress.org/terms/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Zhao, Lei
Hou, Yuqing
Picariello, Tyler
Craige, Branch
Witman, George B.
Proteome of the central apparatus of a ciliary axoneme
title Proteome of the central apparatus of a ciliary axoneme
title_full Proteome of the central apparatus of a ciliary axoneme
title_fullStr Proteome of the central apparatus of a ciliary axoneme
title_full_unstemmed Proteome of the central apparatus of a ciliary axoneme
title_short Proteome of the central apparatus of a ciliary axoneme
title_sort proteome of the central apparatus of a ciliary axoneme
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548120/
https://www.ncbi.nlm.nih.gov/pubmed/31092556
http://dx.doi.org/10.1083/jcb.201902017
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